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2011 | 58 | 2 | 199-202
Article title

Metabolism of bradykinin in aorta of hypertensive rats

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EN
Abstracts
EN
Alterations in the formation and metabolism of bradykinin (Bk) are hypothesized to play a role in the pathophysiology of hypertension, atherosclerosis and vascular complications of diabetes. However, despite its prominent role in cardiovascular regulation, studies on bradykinin have been limited by various difficulties in accurate measurements of this peptide in biological samples. In this study, using the LC-ESI-MS method we estimated the conversion of exogenous Bk to its main metabolites - Bk-(1-5) and Bk-(1-7) - in endothelial cell culture and in fragments of aorta of normotensive (WKY) and hypertensive rats (SHR). The effects of angiotensin converting enzyme (ACE) and neutral endopeptidase (NEP) inhibitors were more pronounced in SHR: perindoprilat inhibited Bk-(1-5) formation by 49 % and 76 % in WKY and SHR rats, respectively, and tiorphan tended to decrease formation of Bk-(1-5) in both groups of animals. The degradation of bradykinin and generation of both metabolites were significantly higher in the aorta of SHR rats than in WKY controls. Our results show that even in relatively early hypertension (in 4-month old SHR rats) inactivation of Bk by aorta wall is enhanced.
Publisher

Year
Volume
58
Issue
2
Pages
199-202
Physical description
Dates
published
2011
received
2010-10-06
revised
2011-04-29
accepted
2011-05-04
(unknown)
2011-05-27
Contributors
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
author
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
author
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
author
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
  • Chair of Pharmacology, Jagiellonian University Medical College, Kraków, Poland
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv58p199kz
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