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2011 | 58 | 1 | 39-44
Article title

Was the serine protease cathepsin G discovered by S. G. Hedin in 1903 in bovine spleen?

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Abstracts
EN
In the beginning of the 20th century, enzymes with proteolytic activity were classified as peptidases, Erepsin, and proteases. Among these, pepsin, trypsin, and autolytic enzymes were of the protease class. Spleen-derived proteases were poorly characterized until Sven Gustaf Hedin performed several digestion experiments with bovine spleen. He incubated minced bovine spleen under acidic or neutral conditions and characterized two active proteases; the results were published in 1903. The first protease was named α-protease and was active under neutral conditions. The second was named β-protease and was active under acidic conditions. We replicated Hedin's experiments according to his methods and found, by using activity-based probes to visualize proteases, that the historical α-protease is the present-day serine protease cathepsin G (CatG), which is known to be important in several immune processes, including antigen processing, chemotaxis, and activation of surface receptors. The β-protease, however, comprised different proteases including CatX, B, S, and D. We suggest that Hedin described CatG activity in bovine spleen over 100 years ago.
Keywords
Year
Volume
58
Issue
1
Pages
39-44
Physical description
Dates
published
2011
received
2010-07-02
revised
2010-11-10
accepted
2010-12-13
(unknown)
2011-03-07
References
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Publication order reference
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bwmeta1.element.bwnjournal-article-abpv58i1p39kz
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