Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase

• Authors: Marta Zalewska , Andrzej Ożyhar , Marian Kochman
• Languages of publication: EN

Abstracts

EN

Juvenile hormone (JH) is essential for multiple physiological processes: it controls larval development, metamorphosis and adult reproduction. In insect hemolymph more than 99 % of JH is bound to juvenile hormone binding protein (JHBP), which protects JH from degradation by nonspecific hydrolases and serves as a carrier to supply the hormone to the target tissues. In Galleria mellonella hemolymph, JHBP is found in a complex with lipid-binding high molecular weight proteins (HMWP) and this interaction is enhanced in the presence of JH. In this report, we present studies on the interaction of JHBP with low molecular weight proteins (LMWP) in the hemolymph. Using ligand blotting we found that JHBP interacts with a protein of about 44 kDa. To identify the protein that preferentially binds JHBP, a LMWP fraction was applied to a Sepharose-bound JHBP and, after washing, the column was eluted with free JHBP acting as a specific competitor or with carbonic anhydrase as a negative control. The eluted proteins were separated by SDS/PAGE and analyzed by mass spectrometry. Isocitrate dehydrogenase was identified as a component of the supramolecular complex of JHBP with hemolymph proteins.

Keywords

EN

protein-protein interaction; JHBP

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2011
• Volume: 58
• Issue: 1
• Pages: 119-124

Dates

published

2011

received

2010-09-01

revised

2010-12-23

accepted

2010-12-24

(unknown)

2011-03-14

Contributors

author

Marta Zalewska

• Department of Biochemistry, Faculty of Chemistry, Wroclaw University of Technology, Wrocław, Poland

author

Andrzej Ożyhar

• Department of Biochemistry, Faculty of Chemistry, Wroclaw University of Technology, Wrocław, Poland

author

Marian Kochman

• Department of Biochemistry, Faculty of Chemistry, Wroclaw University of Technology, Wrocław, Poland

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