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2010 | 57 | 1 | 89-93
Article title

One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides

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EN
Abstracts
EN
A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90%. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.
Publisher

Year
Volume
57
Issue
1
Pages
89-93
Physical description
Dates
published
2010
received
2009-12-01
revised
2010-02-15
accepted
2010-03-11
(unknown)
2010-03-16
Contributors
  • Department of Molecular and Medical Biophysics, Medical University of Łódź, Łódź, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p89kz
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