Regulation of subcellular localization of muscle FBPase in cardiomyocytes. The decisive role of calcium ions

• Authors: Michal Majkowski , Dorota Wypych , Pawel Pomorski , Andrzej Dzugaj
• Languages of publication: EN

Abstracts

EN

Glyconeogenesis, the synthesis of glycogen from carbohydrate precursors like lactate, seems to be an important pathway participating in replenishing glycogen in cardiomyocytes. Fructose-1,6-bisphosphatase (FBPase), an indispensible enzyme of glyconeogenesis, has been found in cardiomyocytes on the Z-line, in the nuclei and in the intercalated discs. Glyconeogenesis may proceed only when FBPase accumulates on the Z-line. Searching for the mechanism of a FBPase regulation we investigated the effects of the calcium ionophore A23187, a muscle relaxant dantrolene, glucagon, insulin and medium without glucose on the subcellular localization of this enzyme in primary culture of neonatal rat cardiomyocytes. Immunofluorescence was used for protein localization and the intracellular calcium concentration was measured with Fura. We found that the concentration of calcium ions was the decisive factor determining the localization of muscle FBPase on the Z-line. Calcium ions had no effect on the localization of the enzyme in the intercalated discs or in the nuclei, but accumulation of FBPase in the nuclei was induced by insulin.

Keywords

EN

cardiomyocytes; Z-line; insulin; calcium; intercalated disc; muscle FBPase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 4
• Pages: 597-605

Dates

published

2010

received

2010-08-30

accepted

2010-10-27

(unknown)

2010-11-02

Contributors

author

Michal Majkowski

• Department of Cytobiochemistry, Faculty of Biotechnology, Wrocław, Poland

author

Dorota Wypych

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Pawel Pomorski

• Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland

author

Andrzej Dzugaj

• Institute of Genetics and Microbiology, University of Wroclaw, Wrocław, Poland

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