Effect of tunicamycin on the biogenesis of hepatitis C virus glycoproteins

• Authors: Natalia Reszka , Ewelina Krol , Arvind H Patel , Boguslaw Szewczyk
• Languages of publication: EN

Abstracts

EN

Hepatitis C virus (HCV) infects humans, with a prevalence around 3% of population, causing acute and chronic hepatitis and hepatocellular carcinoma. We studied the effect of inhibition of glycosylation on the assembly of the HCV particle. HCV possesses two envelope glycoproteins E1 and E2 that are highly modified by N-glycans. These glycan residues are crucial for viral entry and maturation of the progeny. Here, we examined the influence of inhibition of N-glycosylation on expression of E1 and E2. Since the propagation of HCV in cell culture is limited, we used a recombinant baculovirus producing viral-like particles in insect cells. Our data showed that blocking of N-glycan transfer to the nascent polypeptide chain with the antibiotic tunicamycin resulted in the loss of E1 and E2. We also found that a dose of tunicamycin that did not influence the cell viability significantly reduced the E2 level in infected cells. The results indicate that blocking of glycosylation at an early step efficiently reduces the assembly of HCV virions. Thus, we suggest that derivatives of tunicamycin that preferentially block glycosylation of viral proteins may become potential therapeutic agents against HCV.

Keywords

EN

glycoproteins; glycosylation inhibition; hepatitis C virus; tunicamycin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 4
• Pages: 541-546

Dates

published

2010

received

2010-05-10

revised

2010-10-18

accepted

2010-11-27

(unknown)

2010-12-06

Contributors

author

Natalia Reszka

• Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

author

Ewelina Krol

• Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

author

Arvind H Patel

• MRC - University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow, United Kingdom

author

Boguslaw Szewczyk

• Department of Molecular Virology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland

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