Comparative proteomic analysis of Bombyx mori hemolymph and fat body after calorie restriction

• Authors: Huiqing Chen , Yijia Li , Keping Chen , Qin Yao , Guohui Li , Lin Wang
• Languages of publication: EN

Abstracts

EN

Calorie restriction (CR) is known to extend life span from yeast to mammals. To gain an insight into the effects of CR on growth and development of the silkworm Bombyx mori at protein level, we employed comparative proteomic approach to investigate proteomic differences of hemolymph and fat body of the silkworm larvae subjected to CR. Thirty-nine differentially expressed proteins were identified by MALDI TOF/TOF MS. Among them, 19 were from the hemolymph and 20 from the fat body. The hemolymph of the CR group contained two down-regulated and 17 up-regulated proteins, whereas the fat body contained 15 down-regulated and five up-regulated ones. These proteins belonged to those functioning in immune system, in signal transduction and apoptosis, in regulation of growth and development, and in energy metabolism. Our results suggest that CR can alter the expression of proteins related to the above four aspects, implying that these proteins may regulate life span of the silkworm through CR.

Keywords

EN

calorie restriction; Bombyx mori; two-dimensional gel electrophoresis; proteomic analysis; MALDI-TOF/TOF MS

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 4
• Pages: 505-511

Dates

published

2010

received

2010-03-05

revised

2010-10-07

accepted

2010-10-24

(unknown)

2010-11-02

Contributors

author

Huiqing Chen

• Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China

author

Yijia Li

• Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China

author

Keping Chen

• Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China

author

Qin Yao

• Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China

author

Guohui Li

• Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China

author

Lin Wang

• Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, P. R. China

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