Reversion of argE3 to Arg+ in Escherichia coli AB1157 -an informative bacterial system for mutation detection

• Authors: Anna Sikora , Elżbieta Grzesiuk
• Languages of publication: EN

Abstracts

EN

This review concerns reversion of the argE3 (ochre) nonsense mutation to prototrophy in E. coli AB1157 strain as an informative system for mutation detection. Strain AB1157 bears the argE3 (ochre), hisG4 (ochre) and thr-1 (amber) mutations, and the supE44 amber suppressor on its chromosome. The Arg+ phenotype can be restored by (i) any base substitution at the argE3 site that changes the nonsense UAA codon to any sense nucleotide triplet or to UAG recognized by the supE44 amber suppressor, or (ii) suppressor mutations enabling the reading of the UAA nonsense codon. The argE3 → Arg+ reversion-based system enables (i) determination of the spontaneous or induced mutation level; (ii) determination of base substitutions (suppressor analysis); (iii) examination of transcription-coupled repair (TCR) since targets for DNA damage are situated on the transcribed or coding strand of DNA; (iv) detection of mutations resulting from single stranded DNA damage. This review focuses on studies carried out since the early 1990s till now with the application of the AB1157-based mutation detection system. Recently, the system has been used to obtain new data on the processes of methyl methanesulfonate-induced mutagenesis and DNA repair in E. coli alkB- mutants.

Keywords

EN

mutations; Arg+ revertants; Escherichia coli K12; prototrophy; T4 phages; suppressor tRNA

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 4
• Pages: 479-485

Dates

published

2010

received

2010-07-12

revised

2010-10-06

accepted

2010-10-21

(unknown)

2010-10-27

Contributors

author

Anna Sikora

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Elżbieta Grzesiuk

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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