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2010 | 57 | 4 | 409-412
Article title

Tissue variation of mitochondrial oxidative phosphorylation efficiency in cold-acclimated ducklings

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We investigated the oxidative phosphorylation efficiency of liver and gastrocnemius muscle mitochondria in thermoneutral and cold-acclimated ducklings. The yield of oxidative phosphorylation was lower in muscle than in liver mitochondria, a difference that was associated with a higher proton conductance in muscle mitochondria. Cold exposure did not affect oxidative phosphorylation efficiency or basal proton leak in mitochondria. We conclude that the basal proton conductance of mitochondria may regulate mitochondrial oxidative phosphorylation efficiency, but is not an important contributor to thermogenic processes in cold-acclimated ducklings.

Physical description
  • Ecologie des Hydrosystèmes fluviaux, UMR 5023 CNRS, Université de Lyon, Villeurbanne, France
  • Physiologie Intégrative, Cellulaire et Moléculaire, UMR 5123 CNRS, Université de Lyon, Villeurbanne, France
  • Biométrie et Biologie Evolutive, UMR 5558 CNRS, Université de Lyon, Villeurbanne, France
  • Physiologie Intégrative, Cellulaire et Moléculaire, UMR 5123 CNRS, Université de Lyon, Villeurbanne, France
  • Ecologie des Hydrosystèmes fluviaux, UMR 5023 CNRS, Université de Lyon, Villeurbanne, France
  • Physiologie Intégrative, Cellulaire et Moléculaire, UMR 5123 CNRS, Université de Lyon, Villeurbanne, France
  • Physiologie Intégrative, Cellulaire et Moléculaire, UMR 5123 CNRS, Université de Lyon, Villeurbanne, France
  • Bedu E, Cohen-Adad F, Dallevet G, Garin D, Barre H, Duchamp C (2001) Effect of cold-acclimation on oxygen uptake and glucose production of perfused duckling liver. Comp Biochem Physiol A Mol Integr Physiol 128: 851-861.
  • Bedu E, Chainier F, Sibille B, Meister R, Dallevet G, Garin D, Duchamp C (2002) Increased lipogenesis in isolated hepatocytes from cold-acclimated ducklings. Am J Physiol Regul Integr Comp Physiol 283: R1245-R1253.
  • Bergmeyer HU (1974) In Methods of enzymatic analysis. Verlag Chemie Weinheim Academic Press, Inc., New York.
  • Brand MD (1995) Measurement of mitochondrial proton motive force. In Bioenergetics - a practical approach, Brown GC, Cooper CE, eds, pp 39-62. IRL Press, Oxford.
  • Brand MD (2005) The efficiency and plasticity of mitochondrial energy transduction. Biochem Soc Trans 33: 897-904.
  • Brand MD, Pakay JL, Ocloo A, Kokoszka J, Wallace DC, Brookes PS, Cornwall EJ (2005) The basal proton conductance of mitochondria depends on adenine nucleotide translocase content. Biochem J 392: 353-362.
  • Chaînier F, Roussel D, Georges B, Meister R, Rouanet JL, Duchamp C, Barré H (2000) Cold acclimation or grapeseed oil feeding affects phospholipid composition and mitochondrial function in duckling skeletal muscle. Lipids 35: 1099-1106.
  • Collin A, Buyse J, van As P, Darras VM, Malheiros RD, Moraes VMB, Reyns GE, Taouis M, Decuypere E (2003) Cold-induced enhancement of avian uncoupling protein expression, heat production, and triiodothyronine concentrations in broiler chicks. Gen Comp Endocrinol 130: 70-77.
  • Duchamp C, Barre H (1993) Skeletal-muscle as the major site of nonshivering thermogenesis in cold-acclimated ducklings. Am J Physiol 265: R1076-R1083.
  • Duchamp C, Marmonier F, Denjean F, Lachuer J, Eldershaw TPD, Rouanet JL, Morales A, Meister R, Benistant C, Roussel D et al. (1999) Regulatory, cellular and molecular aspects of avian muscle nonshivering thermogenesis. Ornis Fenn 76: 151-165.
  • Duchamp C, Cohen Adad F, Rouanet JL, Barre H (1992) Histochemical arguments for muscular nonshivering thermogenesis in muscovy ducklings. J Physiol 457: 27-45.
  • Dumonteil E, Barre H, Meissner G (1995) Expression of sarcoplasmic-reticulum Ca2+ transport proteins in cold-acclimating ducklings. Am J Physiol 269: C955-C960.
  • Hulbert AJ, Turner N, Hinde J, Else P, Guderley H (2006) How might you compare mitochondria from different tissues and different species? J Comp Physiol B 176: 93-105.
  • Mollica AP, Lionetti L, Crescenzo R, Tasso R, Barletta A, Liverini G, Iossa S (2005) Cold exposure differently influences mitochondrial energy efficiency in rat liver and skeletal muscle. FEBS Lett 579: 1978-1982.
  • Raimbault S, Dridi S, Denjean F, Lachuer J, Couplan E, Bouillaud F, Bordas A, Duchamp C, Taouis M, Ricquier D (2001) An uncoupling protein homologue putatively involved in facultative muscle thermogenesis in birds. Biochem J 353: 441-444.
  • Rey B, Roussel D, Romestaing C, Belouze M, Rouanet JL, Desplaches D, Sibille B, Servais S, Duchamp C (2010) Up-regulation of avian uncoupling protein in cold-acclimated and hyperthyroid ducklings prevents reactive oxygen species production by skeletal muscle mitochondria. BMC Physiol 10: 5.
  • Rolfe DFS, Hulbert AJ, Brand MD (1994) Characteristics of mitochondrial proton leak and control of oxidative-phosphorylation in the major oxygen-consuming tissues of the rat. Biochim Biophys Acta 1188: 405-416.
  • Rossignol R, Letellier T, Malgat M, Rocher C, Mazat JP (2000) Tissue variation in the control of oxidative phosphorylation: implication for mitochondrial diseases. Biochem J 347: 45-53.
  • Roussel D, Rouanet JL, Duchamp C, Barre H (1998) Effects of cold acclimation and palmitate on energy coupling in duckling skeletal muscle mitochondria. FEBS Lett 439: 258-262.
  • Roussel D, Harding M, Runswick MJ, Walker JE, Brand MD (2002) Does any yeast mitochondrial carrier have a native uncoupling protein function? J Bioenerg Biomembr 34: 165-176.
  • Skulachev VP, Maslov SP (1960) The role of nonphosphorylating oxidation in temperature regulation. Biochemistry (Moscow) 25: 826-832.
  • Stock D, Leslie AGW, Walker JE (1999) Molecular architecture of the rotary motor in ATP synthase. Science 286: 1700-1705.
  • Stuart JA, Cadenas S, Jekabsons MB, Roussel D, Brand MD (2001) Mitochondrial proton leak and the uncoupling protein 1 homologues. Biochim Biophys Acta 1504: 144-158.
  • Talbot DA, Hanuise N, Rey B, Rouanet JL, Duchamp C, Brand MD (2003) Superoxide activates a GDP-sensitive proton conductance in skeletal muscle mitochondria from king penguin (Aptenodytes patagonicus). Biochem Biophys Res Commun 312: 983-988.
  • Talbot DA, Duchamp C, Rey B, Hanuise N, Rouanet JL, Sibille B, Brand MD (2004) Uncoupling protein and ATP/ADP carrier increase mitochondrial proton conductance after cold adaptation of king penguins. J Physiol 558: 123-135.
  • Teulier L, Rouanet JL, Letexier D, Romestaing C, Belouze M, Rey B, Duchamp C, Roussel D (2010) Cold-acclimation-induced non-shivering thermogenesis in birds is associated with upregulation of avian UCP but not with innate uncoupling or altered ATP efficiency. J Exp Biol 213: 2476-2482.
  • Toyomizu M, Ueda M, Sato S, Seki Y, Sato K, Akiba Y (2002) Cold-induced mitochondrial uncoupling and expression of chicken UCP and ANT mRNA in chicken skeletal muscle. FEBS Lett 529: 313-318.
  • Vianna CR, Hagen T, Zhang CY, Bachman E, Boss O, Gereben B, Moriscot AS, Lowell BB, Bicudo J, Bianco AC (2001) Cloning and functional characterization of an uncoupling protein homolog in hummingbirds. Physiol Genomics 5: 137-145.
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