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2010 | 57 | 4 | 403-408
Article title

Fluorescent in situ hybridization of mitochondrial DNA and RNA

Content
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EN
Abstracts
EN
To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.
Year
Volume
57
Issue
4
Pages
403-408
Physical description
Dates
published
2010
received
2010-08-30
revised
2010-11-04
accepted
2010-11-25
(unknown)
2010-11-29
Contributors
author
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
author
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
author
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p403kz
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