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2010 | 57 | 4 | 403-408
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Fluorescent in situ hybridization of mitochondrial DNA and RNA

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To reveal nucleic acid localization in mitochondria, we designed molecular beacon fluorescent probes against: i) the light strand complementary to ND5 mitochondrial DNA (mtDNA) gene (annealing also to corresponding mRNA); ii) displacement (D) loop 7S DNA (annealing also to parallel heavy strand mtDNA and corresponding light strand transcript); iii) the proximal D-loop heavy strand displaced by the light strand promoter minor RNA. Confocal microscopy demonstrated ND5 probe spreading (less for other probes) in mitochondrial reticulum tubules but upon RNase A treatment all probes contoured mtDNA nucleoid localization. DNase I spread the signal over mitochondrial tubules. Future applications are discussed.
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  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Department No. 75, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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