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2010 | 57 | 3 | 379-384
Article title

Conjugated linoleic acids regulate triacylglycerol and cholesterol concentrations in macrophages/foam cells by the modulation of CD36 expression

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EN
Abstracts
EN
Atherosclerosis is an inflammatory disease characterised by the accumulation of lipids and their metabolites in the artery wall. During inflammation circulating LDL are taken up by macrophages through two major scavenger receptors: CD36 and scavenger receptor A (SRA). Fatty acids that are common in food, e.g. linoleic acid and n-3 unsaturated fatty acids can modulate expression of CD36 on the macrophage surface. Conjugated linoleic acid isomers (CLA) that originate from the human diet, have demonstrated antiatherogenic properties in several experiments. Animal study evidenced that CLA could induce resolution of plaque by activation of peroxisome proliferator activated receptors and down-regulation of pro-inflammatory genes. Less unequivocal results were obtained in human studies (on the CLA effects on the inflammatory process). Therefore in this study we investigated the influence of CLA on CD36 expression and lipid accumulation in human macrophages. Macrophages were incubated with 30 µM cis-9,trans-11 CLA, trans-10,cis-12 CLA or linoleic acid for 48 h. After that, expression of CD36 as well as accumulation of lipids were measured by flow cytometry, microscopy and a spectroscopic method. We demonstrate that both cis-9,trans-11 C 18:2 CLA and linoleic acid slightly elevated expression of CD36, whereas second isomer - trans-10,cis-12 CLA - did not. Nevertheless, only trans-10,cis-12 CLA triggered delipidation of macrophages, that is decreased triacylglycerols concentration. Also in human adipocytes, trans-10,cis-12 CLA causes cell delipidation by reduction of PPAR receptor expression. We propose a similar mechanism for human macrophages/foam cells.
Keywords
Publisher

Year
Volume
57
Issue
3
Pages
379-384
Physical description
Dates
published
2010
received
2009-10-28
revised
2010-04-22
accepted
2010-05-21
(unknown)
2010-06-09
Contributors
  • Department of Biochemistry and Human Nutrition, Pomeranian Medical University, Szczecin, Poland
  • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
  • Department Histology and Embryology, Pomeranian Medical University, Szczecin, Poland
  • Department Histology and Embryology, Pomeranian Medical University, Szczecin, Poland
  • Department of Clinical and Molecular Medicine, Pomeranian Medical University, Szczecin, Poland
  • Department Histology and Embryology, Pomeranian Medical University, Szczecin, Poland
  • Department of General Pathology, Pomeranian Medical University, Szczecin, Poland
  • Department of Biochemistry, Pomeranian Medical University, Szczecin, Poland
References
  • AbuGhazaleh AA, Schingoethe DJ, Hippen AR, Kalscheur KF (2003) Conjugated linoleic acid and vaccenic acid in rumen, plasma, and milk of cows fed fish oil and fats differing in saturation of 18 carbon fatty acids. J Dairy Sci 86: 3648-3660.
  • Badinga L, Greene ES (2006) Physiological properties of conjugated linoleic acid and implications for human health. Nutr Clin Pract 21: 367-363.
  • Bailllie AGS, Coburn CT, Abumrad NA (1996) Reversible binding of long-chain fatty acids to purified FAT, the adipose CD36 homolog. J Membr Biol 153: 75-81.
  • Brown JM, Boysen MS, Jensen SS, Morrison RF, Storkson J, Lea-Currie R, Pariza M, Mandrup S, McIntosh MK (2003) Isomer specific regulation of metabolism and PPAR gamma signalling by CLA in human preadipocytes. J Lipid Res 44: 1287-1300.
  • Collot-Texiera S, Martin J, McDermott-Roe C, Poston R, McGregor JL (2007) CD36 and macrophages in atherosclerosis. Cardiovasc Res 75: 468-477.
  • Cynshi O, Takashoma Y, Suzuki T, Kawabe Y, Ohba Y, Kodama T (1994) Characterization of aggregated low density lipoproteins induced by copper-catalyzed oxidation. J Atheroscler Thromb 1: 87-96.
  • Evans M, Geigerman C, Cook J, Curtis L, Kuebler B, McIntosh M (2001a) Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids 35: 899-910.
  • Evans M, Park Y, Pariza M, Curtis L, Kuebler B, McIntosh M (2001b) Trans-10, cis-12 conjugated linoleic acid reduces triglyceride content while differentially affecting peroxisome proliferator activated receptor gamma 2 and aP2 expression in 3T3-L1 preadipocytes. Lipids 36: 1223-1232.
  • Havel RJ, Eder HA, Bragdon JA (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest 34: 1345-1353.
  • Kliewer SA, Lehmann JM, Willson TM (1999) Orphan nuclear receptors: shifting endocrinology into reverse. Science 284: 757-760.
  • Lee KN, Kritchevsky D, Pariza MW (1994) Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108: 19-21.
  • Lee SH, Yamaguhi K, Kim JS, Elinq TE (2006a) Conjugated linoleic acid stimulates an anti-tumorigenic protein NAG-1 in an isomer specific manner. Carcinogenesis 27: 972-981.
  • Lee SH, Yamaguhi K, Kim JS, Elinq TE (2006b) Conjugated linoleic acid stimulates an anti-tumorigenic protein NAG-1 in an isomer specific manner. Carcinogenesis 27: 972-981.
  • Lee KW, Lee HJ, Cho HY, Kim YJ (2005) Role of the conjugated linoleic acid in the prevention of cancer. Crit Rev Food Sci Nutr 45: 135-144.
  • Nagy L, Tontonoz P, Alvarez JG, Chen H, Evans RM (1998) Oxidized LDL regulates macrophage gene expression through ligand activation of PPARgamma. Cell 93: 229-240.
  • Park SJ, Park CW, Kim SJ, Kim JK, Kim YR, Park KA, Kim JO, Ya YL (2002) Methylation methods for the quantitative analysis of conjugated linoleic acid (CLA) isomers in various lipid samples. J Agric Food Chem 50: 989-986.
  • Pietsch A, Weber C, Goretzki M, Weber PC, Lorenz RL (1995) N-3 but not N-6 fatty acids reduce the expression of combined adhesion and scavenger receptor CD36 in human monocytic cells. Cell Biochem Funct 13: 211-216.
  • Prieur X, Roszer T, Rocote M (2009) Lipotoxicity in macrophages: evidence from diseases associated with the metabolic syndrome. Biochim Biophys Acta doi.1016/j.bbalip.2009.09.017.
  • Rahman MM, Bhattacharya A, Fernandes GJ (2006) Conjugated linoleic acid inhibits osteoclast differentiation of RAW 264.7 cells by modulating RANKL signalling. J Lipid Res 47: 1739-1748.
  • Ringseis R, Wen G, Saal D, Eder K (2008) Conjugated linoleic acid isomers reduce cholesterol accumulation in acetylated LDL-induced mouse RAW264.7 macrophage-derived foam cells. Lipids 43: 913-923.
  • Stachowska E, Baśkiewicz-Masiuk M, Dziedziejko V, Adler G, Bober J, Machaliński B, Chlubek D (2007) Conjugated linoleic acids can change phagocythosis of human monocytes/macrophages by reduction in Cox-2 expression. Lipids 42: 707-716.
  • Stachowska E, Baśkiewicz-Masiuk M, Dziedziejko V, Gutowska I, Baranowska-Bosiacka I, Marchlewicz M, Dołegowska B, Wiszniewska B, Machaliński B, Chlubek D (2008) Conjugated linoleic acid increases intracellular ROS synthesis and oxygenation of arachidonic acid in macrophages. Nutrition 24: 187-189.
  • Taketa K, Matsumura T, Yano M, Ishii N, Senokuchi T, Motoshima H, Murata Y, Kim-Mitsuyama S, Kawada T, Itabe H, Takeya M, Nishikawa T, Tsuruzoe K, Araki E (2008) Oxidized low density lipoprotein activates peroxisome proliferator-activated receptor-alpha (PPAR alpha) and PPAR gamma through MAPK-dependent MAPK-dependent COX-2 expression in macrophages. J Biol Chem 283: 9852-9862.
  • Teupser D, Mueller MA, Koglin J, Wilfert W, Ernst J, von Scheidt W, Steinbeck G, Seidel D, Thiery J (2008) CD 36 mRNA expression in increased in CD14+monocytes of patients with coronary heart disease. Clin Exp Pharmacol Physiol 35: 552-556.
  • Tontonoz P, Nagy L, Alvarez JG, Thomazy VA, Evans RM (1998) PPARgamma promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93: 241-252.
  • Toomey S, Roche H, Fitzgerald D, Belton O (2003) Regression of pre-established atherosclerosis in the apoE-/- mouse by conjugated linoleic acid. Biochem Soc Trans 31: 1075-1079.
  • Vallvé JC, Uliaque K, Girona J, Cabré A, Ribalta J, Heras M, Masana L (2002) Unsaturated fatty acids and their oxidation products stimulate gene CD36 expression in human macrophages. Atherosclerosis 164: 45-56.
  • Whigham LD, Cook EB, Stahl JL, Saban R, Bjorling DE, Pariza MW, Cook ME (2001) CLA reduces antigen-induced histamine and PGE(2) release from sensitized guinea pig tracheae. Am J Physiol Regul Integr Comp Physiol 280: R908-R912.
  • Xie S, Lee YF, Kim E, Chen LM, Ni J, Fang LY, Liu S, Lin SJ, Abe J, Berk B, Ho FM, Chang C (2009) TR4 nuclear functions as a fatty acid sensor to modulate CD36 expression and foam cell formation. Proc Natl Acad Sci USA 106: 13353-13358.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p379kz
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