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2010 | 57 | 3 | 361-368

Article title

Salivary aldehyde dehydrogenase - temporal and population variability, correlations with drinking and smoking habits and activity towards aldehydes contained in food


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Fluorimetric method based on oxidation of the fluorogenic 6-methoxy-2-naphthaldehyde was applied to evaluate temporal and population variability of the specific activity of salivary aldehyde dehydrogenase (ALDH) and the degree of its inactivation in healthy human population. Analyzed was also its dependence on drinking and smoking habits, coffee consumption, and its sensitivity to N-acetylcysteine. Both the specific activity of salivary ALDH and the degree of its inactivation were highly variable during the day, with the highest activities recorded in the morning hours. The activities were also highly variable both intra- and interpersonally, and negatively correlated with age, and this correlation was stronger for the subgroup of volunteers declaring abstinence from alcohol and tobacco. Moderately positive correlations of salivary ALDH specific activity with alcohol consumption and tobacco smoking were also recorded (rs ~0.27; p=0.004 and rs =0.30; p=0.001, respectively). Moderate coffee consumption correlated positively with the inactivation of salivary ALDH, particularly in the subgroup of non-drinking and non-smoking volunteers. It was found that mechanical stimulation of the saliva flow increases the specific activity of salivary ALDH. The specific activity of the salivary ALDH was strongly and positively correlated with that of superoxide dismutase, and somewhat less with salivary peroxidase. The antioxidant-containing drug N-acetylcysteine increased activity of salivary ALDH presumably by preventing its inactivation in the oral cavity. Some food-related aldehydes, mainly cinnamic aldehyde and anisaldehyde, were excellent substrates of the salivary ALDH3A1 enzyme, while alkenals, particularly those with short chain, were characterized by lower affinity towards this enzyme but high catalytic constants. The protective role of salivary ALDH against aldehydes in food and those found in the cigarette smoke is discussed, as well as its participation in diminishing the effects of alcohol- and smoking-related oxidative stress.








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  • Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warszawa, Poland
  • Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warszawa, Poland
  • Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, Warszawa, Poland
  • Department of Biophysics, Faculty of Food Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Department of Biophysics, Faculty of Food Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Department of Biophysics, Faculty of Food Sciences, University of Warmia and Mazury, Olsztyn, Poland
  • Department of Biophysics, Faculty of Food Sciences, University of Warmia and Mazury, Olsztyn, Poland


  • Adams TB, Doull J, Goodman JI, Munro IC, Newberne P, Portoghese PS, Smith RL, Wagner BM, Weil CS, Woods LA, Ford RA (1997) The FEMA GRAS assessment of furfural used as a flavour ingredient. Food Chem Toxicol 35: 739-751.
  • Adams TB, Cohen SM, Doull J, Feron VJ, Goodman JI, Marnett LJ, Munro IC, Portoghese PS, Smith RL, Waddell WJ, Wagner BM (2005) The FEMA GRAS assessment of phenethyl alcohol, aldehyde, acid, and related acetals and esters used as flavor ingredients. Food Chem Toxicol 43: 1179-1206.
  • Adams TB, Gavin CL, Taylor SV, Waddell WJ, Cohen SM, Feron, VJ, Goodman J, Rietjens IMCM, Marnett LJ, Portoghese PS, Smith RL (2007) The FEMA GRAS assessment of α,β-unsaturated aldehydes and related substances used as flavor ingredients. Food Chem Toxicol 46: 2935-2967.
  • Ashby MT (2008) Inorganic chemistry of defensive peroxidases in the human oral cavity. J Dent Res 87: 900-914.
  • Atkuri KR, Mantovani JJ, Herzenberg LA, Herzenberg (2007) N-acetylcysteine - a safe antidote for cysteine/glutathione deficiency. Curr Opin Pharmacol 7: 355-359.
  • Bogucka M, Giebułtowicz J, Zawada K, Wroczyński P, Wierzchowski J, Pietrzak M, Piekarczyk P, Romanowska K (2009) The oxidation status of ALDH3A1 in human saliva and its correlation with antioxidant capacity measured by ORAC method. Acta Pol Pharm 66: 477-482.
  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem 44: 276-287.
  • Casetta I, Govoni V, Granieri E (2005) Oxidative stress, antioxidants and neurodegenerative diseases. Curr Pharm Des 11: 2033-2052.
  • Das SK, Vasudevan DM (2007) Alcohol-induced oxidative stress. Life Sci 81: 177-187.
  • Dyck LE (1995) Polymorphism of a class 3 aldehyde dehydrogenase present in human saliva and in hair roots. Alcohol Clin Exp Res 19: 420-426.
  • Ellis EM (2007) Reactive carbonyls and oxidative stress: Potential for therapeutic intervention. Pharmacol Ther 115: 13-24.
  • Esser D, Alvarez-Llamas G, de Vries M, Weening D, Vonk RJ, Roelofsen H (2008) Sample stability and protein composition of saliva: Implications for its use as a diagnostic fluid. Biomarker Insights 3: 25-37.
  • Feron VJ, Til HP, de Vrijer F, Woutersen RA, Cassee FR, van Bladeren PJ (1991) Aldehydes: occurrence, carcinogenic potential, mechanism of action and risk assessment. Mutat Res 259: 363-385.
  • Giebułtowicz J, Wolinowska M, Sztybor A, Pietrzak M, Wroczynski P, Wierzchowski J (2009) Salivary aldehyde dehydrogenase: activity towards aromatic aldehydes and comparison with recombinant ALDH3A1. Molecules 14: 2363-2372.
  • Glatt H, Rost K, Frank H, Seidel A, Kollock R (2008) Detoxification of promutagenic aldehydes derived from methylpyrenes by human aldehyde dehydrogenases ALDH2 and ALDH3A1. Arch Biochem Biophys 477: 196-205.
  • Gowder S, Devaraj H (2010) A review of the nephrotoxicity of the food flavor cinnamaldehyde. Curr Bioact Compd 6: 106-117.
  • Hasnis E, Reznick AZ, Pollack S, Klein Y, Nagler RM (2004) Synergistic effect of cigarette smoke and saliva on lymphocytes - The mediatory role of volatile aldehydes and redox active iron and the possible implications for oral cancer. Int J Biochem Cell Biol 36: 826-839.
  • Ihalin R, Loimaranta V, Tenovuo J (2006) Origin, structure, and biological activities of peroxidases in human saliva. Arch Biochem Biophys 445: 261-268.
  • Kasai H, Kawai K (2008) 4-oxo hexenal, a mutagen formed by omega-3 fat peroxidation. Mutat Res 659: 56-59.
  • Lassen N, Black WJ, Estey T, Vasiliou V (2008) The role of corneal crystallins in the cellular defense mechanisms against oxidative stress. Semin Cell Dev Biol 19: 100-112.
  • Moreb SJ, Muhoczy D, Ostmark B, Zucali JR (2007) RNAi-mediated knockdown of aldehyde dehydrogenase class-1A1 and class-3A1 is specific and reveals that each contributes equally to the resistance against 4-hydroperoxycyclophosphamide. Cancer Chemother Pharmacol 59: 127-136.
  • Nagler RM, Klein I, Zarzhevsky N, Drigues N, Reznick AZ (2002) Characterization of the differentiated antioxidant profile of human saliva. Free Radic Biol Med 32: 268-277.
  • Nagler RM, Reznick AZ (2004) Cigarette smoke effects on salivary antioxidants and oral cancer - Novel concepts Isr Med Assoc J 6: 691-694.
  • Pappa A, Chen C, Koutalos Y, Townsend AJ, Vasiliou V (2003a) ALDH3A1 protects human corneal epithelial cells from ultraviolet- and 4-hydroxy-2-nonenal-induced oxidative damage. Free Radic Biol Med 34: 1178-1189.
  • Pappa A, Estey T, Manzer R, Brown D, Vasiliou V (2003b) Human aldehyde dehydrogenase 3A1 (ALDH3A1): Biochemical characterization and immunohistochemical localization in the cornea. Biochem J 376: 615-623.
  • Proctor GB, Chan K (1994) A fluorometric assay of peroxidase utilizing 2',7'-dichlorofluorescin with thiocyanate: application to the study of salivary secretion. J Biochem Biophys Methods 28: 329-336.
  • Salaspuro MP (2003) Alcohol consumption and cancer of the gastrointestinal tract. Best Pract Res Clin Gastroenterol 17: 679-694.
  • Salaspuro MP (2009) Acetaldehyde as a common denominator and cumulative carcinogen in digestive tract cancers. Scand J Gastroenterol 44: 912-925.
  • Seitz HK, Matsuzaki S, Yokoyama A, Homann N, Väkeväinen S, Xian Dong Wang (2001) Alcohol and cancer. Alcohol Clin Exp Res 25: 137S-143S.
  • Sládek NE (1999) Aldehyde dehydrogenase-mediated cellular relative insensitivity to the oxazaphosphorines. Curr Pharm Des 5: 607-625.
  • Sládek NE (2004) Human aldehyde dehydrogenases: Potential pathological, pharmacological, and toxicological impact. J Biochem Mol Toxicol 17: 7-23.
  • Sládek NE, Kollander R, Sreerama L, Kiang DT (2002) Cellular levels of aldehyde dehydrogenases (ALDH1A1 and ALDH3A1) as predictors of therapeutic responses to cyclophosphamide-based chemotherapy of breast cancer: A retrospective study. Cancer Chemother Pharmacol 49: 309-321.
  • Sreerama L, Sládek NE (1996) Over-expression of glutathione S-transferases, DT-diaphorase and an apparently tumour-specific cytosolic class-3 aldehyde dehydrogenase by warthin tumours and mucoepidermoid carcinomas of the human parotid gland. Arch Oral Biol 41: 597-605.
  • Sreerama L, Sládek NE (1994) Identification of a methylcholanthrene-induced aldehyde dehydrogenase in a human breast adenocarcinoma cell line exhibiting oxazaphosphorine-specific acquired resistance. Cancer Res 54: 2176-2185.
  • Sreerama L, Hedge MW, Sládek NE (1995) Identification of a class 3 aldehyde dehydrogenase in human saliva and increased levels of this enzyme, glutathione S-transferases, and DT-diaphorase in the saliva of subjects who continually ingest large quantities of coffee or broccoli. Clin Cancer Res 1: 1153-1165.
  • Townsend AJ, Leone-Kabler S, Haynes RL, Wu Y, Szweda L, Bunting KD (2001) Selective protection by stably transfected human ALDH3A1 (but not human ALDH1A1) against toxicity of aliphatic aldehydes in V79 cells. Chem Biol Interact 130-132: 261-273.
  • Vallari RC, Pietruszko R (1982) Human aldehyde dehydrogenase: Mechanism of inhibition by disulfiram. Science 216: 637-639.
  • Vasiliou V, Pappa A, Estey T (2004) Role of human aldehyde dehydrogenases in endobiotic and xenobiotic metabolism. Drug Metab Rev 36: 279-299.
  • Wierzchowski J, Interewicz E, Wroczynski P, Orlanska I (1995) Continuous fluorimetric assay for human aldehyde dehydrogenase and its application to blood analysis. Anal Chim Acta 319: 209-219.
  • Wierzchowski J, Wroczynski P, Laszuk K, Interewicz E (1997) Fluorimetric detection of aldehyde dehydrogenase activity in human blood, saliva and organ biopsies, and kinetic differentiation between class I and class III isozymes. Anal Biochem 245: 69-78.
  • Wierzchowski J, Pietrzak M, Szeląg M, Wroczyński P (2008) Salivary aldehyde dehydrogenase - reversible oxidation of the enzyme and its inhibition by caffeine, investigated using fluorimetric method. Arch Oral Biol 53: 423-428.
  • Wroczyński P, Wierzchowski J (2000) Aromatic aldehydes as fluorogenic indicators for human aldehyde dehydrogenases and oxidases. Analyst 125: 511-516.
  • Wroczyński P, Wierzchowski J, Rakowska A, Chimkowska M, Targoński J (2004) Aldehyde dehydrogenase in human saliva - evaluation of its oxidation status. Acta Pol Pharm 61 (Suppl): 62-64.

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