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2010 | 57 | 3 | 339-345
Article title

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results

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Abstracts
EN
The aim of this study was to find correlations between folate and vitamin B12 on baseline damage in white blood cells and their association with smoking, alcohol consumption and ageing. Thirty-six healthy vitamin non-deficient male subjects were selected in a randomized study. Comet assay (SCGE) and micronucleus (MN) assay were used as biomarkers of DNA damage. The amount of DNA damage was correlated with vitamin B12 and folic acid concentration. Positive, but non-significant correlation (canonical R = 0.61; χ2=28.97; P=0.253) was found between micronucleus (MN) frequency or comet assay parameters (SCGE) and five covariates (age, smoking, alcohol consumption, vitamin B12 and folate blood serum concentration). The highest MN frequency was observed in the group with the lowest vitamin B12 concentration (F=3.59; P=0.024). The SCGE assay failed to show significant correlation with vitamin B12 or folic acid concentration. Concentration of vitamin B12 was significantly correlated with incidence of micronuclei. Our results present background data that could be valuable for future genotoxicological monitoring.
Publisher

Year
Volume
57
Issue
3
Pages
339-345
Physical description
Dates
published
2010
received
2010-01-29
revised
2010-04-21
accepted
2010-06-08
(unknown)
2010-06-11
Contributors
author
  • Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Laboratory for Radioecology, Ruđer Bošković Institute, Zagreb, Croatia
author
  • Croatian Institute for Transfusion Medicine, Zagreb, Croatia
author
  • Croatian Institute for Transfusion Medicine, Zagreb, Croatia
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p339kz
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