Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

• Authors: Małgorzata Pupek , Anna Lemańska-Perek , Jolanta Jasonek , Iwona Kątnik-Prastowska
• Languages of publication: EN

Abstracts

EN

Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.

Keywords

EN

cerebrospinal fluid; cell-binding fibronectin domain; N-terminal fibronectin domain; C-terminal fibronectin domain; fibronectin

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 3
• Pages: 333-337

Dates

published

2010

received

2010-02-01

revised

2010-05-18

accepted

2010-06-08

(unknown)

2010-08-19

Contributors

author

Małgorzata Pupek

• Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland

author

Anna Lemańska-Perek

• Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland

author

Jolanta Jasonek

• Department and Clinic of Pediatrics and Infectious Diseases, Medical University of Wrocław, Wrocław, Poland

author

Iwona Kątnik-Prastowska

• Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland

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