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2010 | 57 | 3 | 333-337
Article title

Human plasma and cerebrospinal fibronectins differ in the accessibility of the epitopes on the N-terminal domains

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EN
Abstracts
EN
Three monoclonal antibodies specific to the central cell-binding and the C- and N-terminal domains of fibronectin (FN) were used to test antigenic epitope accessibility on human plasma and cerebrospinal fibronectins. In the plasma group, the mean N-terminal FN domain immunoreactivity was about one fourth that of the cell-binding and C-terminal domains, whereas in cerebrospinal fluid they were nearly equal. In the presence of 0.5-6 M urea N-terminal domain immunoreactivity in the plasma increased 3-6-fold, but it decreased 0.7-3-fold in the cerebrospinal fluid. Analysis of fibronectin domain immunoreactivities of the cell-binding and N-terminal domains by a panel of specific monoclonal antibodies may reveal N-terminal fibronectin domain accessibility for reaction with biological partner ligand(s) and/or processes in which FN could be implicated. Such determinations may have important clinical implications.
Publisher

Year
Volume
57
Issue
3
Pages
333-337
Physical description
Dates
published
2010
received
2010-02-01
revised
2010-05-18
accepted
2010-06-08
(unknown)
2010-08-19
Contributors
  • Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland
  • Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland
  • Department and Clinic of Pediatrics and Infectious Diseases, Medical University of Wrocław, Wrocław, Poland
  • Department of Chemistry and Immunochemistry, Medical University of Wrocław, Wrocław, Poland
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Document Type
Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p333kz
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