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2010 | 57 | 3 | 305-311
Article title

Isolation and functional expression of a novel lipase gene isolated directly from oil-contaminated soil

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EN
Abstracts
EN
A lipase gene SR1 encoding an extracellular lipase was isolated from oil-contaminated soil and expressed in Escherichia coli. The gene contained a 1845-bp reading frame and encoded a 615-amino-acid lipase protein. The mature part of the lipase was expressed with an N-terminal histidine tag in E. coli BL21, purified and characterized biochemically. The results showed that the purified lipase combines the properties of Pseudomonas chlororaphis and other Serratia lipases characterized so far. Its optimum pH and temperature for hydrolysis activity was pH 5.5-8.0 and 37°C respectively. The enzyme showed high preference for short chain substrates (556.3±2.8 U/µg for C10 fatty acid oil) and surprisingly it also displayed high activity for long-chain fatty acid. The deduced lipase SR1 protein is probably from Serratia, and is organized as a prepro-protein and belongs to the GXSXG lipase family.
Publisher

Year
Volume
57
Issue
3
Pages
305-311
Physical description
Dates
published
2010
received
2009-10-14
revised
2010-05-14
accepted
2010-08-19
(unknown)
2010-10-02
Contributors
author
  • Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiaotong University, Shanghai, China
author
  • Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiaotong University, Shanghai, China
author
  • Plant Biotechnology Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, College of Agriculture and Life Science, Shanghai Jiaotong University, Shanghai, China
author
  • Key Laboratory for Nuclear Waste Treatment & Environmental Safety (SWUST), College of Science, Technology and Industry for National Defense, Southwest University of Science and Technology, Mianyang, Sichuan, China
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p305kz
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