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2010 | 57 | 3 | 299-304
Article title

Acaconin, a chitinase-like antifungal protein with cytotoxic and anti-HIV-1 reverse transcriptase activities from Acacia confusa seeds

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EN
Abstracts
EN
From the seeds of Acacia confusa, a chitinase-like antifungal protein designated as acaconin that demonstrated antifungal activity toward Rhizoctonia solani with an IC50 of 30±4 µM was isolated. Acaconin demonstrated an N-terminal sequence with pronounced similarity to chitinases and a molecular mass of 32 kDa. It was isolated by chromatography on Q-Sepharose, SP-Sepharose and Superdex 75 and was not bound by either ion exchanger. Acaconin was devoid of chitinase activity. The antifungal activity against Rhizoctonia solani was completely preserved from pH 4 to 10 and from 0°C to 70°C. Congo Red staining at the tips of R. solani hyphae indicated inhibition of fungal growth. However, there was no antifungal activity toward Mycosphaerella arachidicola, Fusarium oxysporum, Helminthosporium maydis, and Valsa mali. Acaconin inhibited proliferation of breast cancer MCF-7 cells with an IC50 of 128±9 µM but did not affect hepatoma HepG2 cells. Its IC50 value toward HIV-1 reverse transcriptase was 10±2.3 µM. The unique features of acaconin include relatively high stability when exposed to changes in ambient pH and temperature, specific antifungal and antitumor actions, potent HIV-reverse transcriptase inhibitory activity, and lack of binding by strongly cationic and anionic exchangers.
Publisher

Year
Volume
57
Issue
3
Pages
299-304
Physical description
Dates
published
2010
received
2009-10-15
revised
2010-06-09
accepted
2010-07-08
(unknown)
2010-08-19
Contributors
author
  • School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
author
  • School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
References
  • Buchner P, Rochat C, Wuillème S, Boutin JP (2002) Characterization of a tissue-specific and developmentally regulated β-1,3-glucanase gene in pea (Pisum sativum). Plant Mol Biol 49: 171-186.
  • Choi AM, Lee SB, Cho SH, Hwang I, Hur CG, Suh MC (2008) Isolation and characterization of multiple abundant lipid transfer protein isoforms in developing sesame (Sesamum indicum L.) seeds. Plant Physiol Biochem 46: 127-139.
  • Chu KT, Ng TB (2005) Purification and characterization of a chitinase-like antifungal protein from black turtle bean with stimulatory effect on nitric oxide production by macrophages. Biol Chem 386: 19-24.
  • Chua AC, Chou WM, Chyan CL, Tzen JT (2007) Purification, cloning, and identification of two thaumatin-like protein isoforms in jelly fig (Ficus awkeotsang) Achenes. J Agric Food Chem 55: 7602-7608.
  • Colussi PA, Specht CA, Taron CH (2005) Characterization of a nucleus-encoded chitinase from the yeast Kluyveromyces lactis. Appl Environ Microbiol 71: 2862-2869.
  • Genta FA, Blanes L, Cristofoletti PT, do Lago CL, Terra WR, Ferreira C (2006) Purification, characterization and molecular cloning of the major chitinase from Tenebrio molitor larval midgut. Insect Biochem Mol Biol 36: 789-800.
  • Ho VS, Ng TB (2007) Chitinase-like proteins with antifungal activity from emperor banana fruits. Protein Pept Lett 14: 828-831.
  • Ikeda M, Miyauchi K, Mochizuki A, Matsumiya M (2009) Purification and characterization of chitinase from the stomach of silver croaker Pennahia argentatus. Protein Expr Purif 65: 214-222
  • Kabir KE, Hirowatari D, Watanabe K, Koga D (2006) Purification and characterization of a novel isozyme of chitinase from Bombyx mori. Biosci Biotechnol Biochem 70: 252-262.
  • Kudan S, Pichyangkura R (2009) Purification and characterization of thermostable chitinase from Bacillus licheniformis SK-1. Appl Biochem Biotechnol 157: 23-35.
  • Laemmli UK, Farve M (1973) Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol 80: 575-599.
  • Lam SK, Ng TB (2001) Isolation of a small chitinase-like antifungal protein from Panax notoginseng (sanchi ginseng) roots. Int J Biochem Cell Biol 33: 287-292.
  • Lam SK, Ng TB (2009) Passiflin, a novel dimeric antifungal protein from seeds of the passion fruit, Phytomedicine 16: 172-180.
  • Lam SK, Han QF, Ng TB (2009) Isolation and characterization of a lectin with potentially exploitable activities from caper (Capparis spinosa) seeds. Biosci Rep 29: 293-299.
  • Lam YW, Wang HX, Ng TB (2000) A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum. Biochem Biophys Res Commun 279: 74-80.
  • Lee TH, Qiu F, Waller GR, Chou CH (2000) Three new flavonol galloylglycosides from leaves of Acacia confusa. J Nat Prod 63: 710-712.
  • Lin JY, Chu SC, Wu HC, Hsieh YS (1991) Trypsin inhibitor from the seeds of Acacia confusa. J Biochem 110: 879-883
  • Lin P, Xia X, Wong JH, Ng TB, Ye XY, Wang S, Shi X (2007) Lipid transfer proteins from Brassica campestris and mung bean surpass mung bean chitinase in exploitability. J Pept Sci 13: 642-648.
  • Liu CL, Shen CR, Hsu FF, Chen JK, Wu PT, Guo SH, Lee WC, Yu FW, Mackey ZB, Turk J, Gross ML (2009) Isolation and identification of two novel SDS-resistant secreted chitinases from Aeromonas schubertii. Biotechnol Prog 25: 124-131.
  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428.
  • Molinari LM, Pedroso RB, Scoaris D de O, Ueda-Nakamura T, Nakamura CV, Dias Filho BP (2007) Identification and partial characterisation of a chitinase from Nile tilapia, Oreochromis niloticus. Comp Biochem Physiol B Biochem Mol Biol 146: 81-87.
  • Moore KG, Price MS, Boston RS (2004) Weissinger, A-K., and Payne, G-A.: A Chitinase from Tex6 Maize Kernels Inhibits Growth of Aspergillus flavus. Phytopathology 94: 82-87.
  • Moreno AB, Martinez Del Pozo A, San Segundo B (2006) Biotechnologically relevant enzymes and proteins. Antifungal mechanism of the Aspergillus giganteus AFP against the rice blast fungus Magnaporthe grisea. Appl Microbiol Biotechnol 72: 883-895.
  • Nguyen NV, Kim YJ, Oh KT, Jung WJ, Park RD (2008) Antifungal activity of chitinases from Trichoderma aureoviride DY-59 and Rhizopus microsporus VS-9. Curr Microbiol 56: 28-32.
  • Nguyen VN, Oh IJ, Kim YJ, Kim KY, Kim YC, Park RD (2009) Purification and characterization of chitinases from Paecilomyces variotii DG-3 parasitizing on Meloidogyne incognita eggs. J Ind Microbiol Biotechnol 36: 195-203.
  • Oshima H, Miyazaki R, Ohe Y, Hayashi H, Kawamura K, Kikuyama S (2002) Isolation and sequence of a novel amphibian pancreatic chitinase. Comp Biochem Physiol B Biochem Mol Biol 132: 381-388.
  • Pan XQ, Shih CC, Harday J (2005) Chitinase induces lysis of MCF-7 cells in culture and of human breast cancer xenograft B11-2 in SCID mice. Anticancer Res 25: 3167-3172.
  • Patil DN, Datta M, Chaudhary A, Tomar S, Sharma AK, Kumar P (2009) Isolation, purification, crystallization and preliminary crystallographic studies of chitinase from tamarind (Tamarindus indica) seeds. Acta Crystallogr Sect F Struct Biol Cryst Commun 65: 343-345.
  • Pegg GF (1998) Chitinase from Verticillium albo-atrum. Methods Enzymol 161: 474-489.
  • Rao DH, Gowda LR (2008) Abundant class III acidic chitinase homologue in tamarind (Tamarindus indica) seed serves as the major storage protein. J Agric Food Chem 56: 2175-2182.
  • Shirota K, Sato T, Sekiguchi J, Miyauchi K, Mochizuki A, Matsumiya M (2008) Purification and characterization of chitinase isozymes from a red algae, Chondrus verrucosus. Biosci Biotechnol Biochem 72: 3091-3099.
  • Taira T, Toma N, Ishihara M (2005) Purification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf. Biosci Biotechnol Biochem 69: 189-196.
  • Tung YT, Wu JH, Huang CC, Peng HC, Chen YL, Yang SC, Chang ST (2009) Protective effect of Acacia confusa bark extract and its active compound gallic acid against carbon tetrachloride-induced chronic liver injury in rats. Food Chem Toxicol 47: 1385-1392.
  • Wang HX, Ng TB (2003) Purification of castamollin, a novel antifungal protein from Chinese chestnuts. Protein Expr Purif 32: 44-51.
  • Wang S, Wu J, Rao P, Ng TB, Ye X (2005) A chitinase with antifungal activity from the mung bean. Protein Expr Purif 40: 230-236.
  • Wang SY, Zhou JJ, Shao B, Lu YJ, Rao PF (2008) A thermostable chitinase with chitin-binding activity from Phaseolus limensis. J Food Sci 73: C452-C457.
  • Wang SL, Chao CH, Liang TW, Chen CC (2009) Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes. Mar Biotechnol (NY) 11: 334-344.
  • Wu JH, Huang CY, Tung YT, Chang ST (2008a) Online RP-HPLC-DPPH screening method for detection of radical-scavenging phytochemicals from flowers of Acacia confusa. J Agric Food Chem 56: 328-332.
  • Wu JH, Tung YT, Chien SC, Wang SY, Kuo YH, Shyur LF, Chang ST (2008b) Effect of phytocompounds from the heartwood of Acacia confusa on inflammatory mediator production. J Agric Food Chem 56: 1567-1573.
  • Yan R, Hou J, Ding D, Guan W, Wang C, Wu Z, Li M (2008) In vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi. J Basic Microbiol 48: 293-301.
  • Ye XY, Ng TB (2002) Delandin, a chitinase-like protein with antifungal, HIV-1 reverse transcriptase inhibitory and mitogenic activities from the rice bean Delandia umbellate. Protein Expr Purif 24: 524-529.
  • Ye XY, Ng TB (2003) Isolation of vulgin, a new antifungal polypeptide with mitogenic activity from the pinto bean. J Pept Sci 9: 114-119.
  • Ye XY, Wang HX, Ng TB (1999) First chromatographic isolation of an antifungal thaumatin-like protein from French bean legumes and demonstration of its antifungal activity. Biochem Biophys Res Commun 263: 130-134.
  • Zhang GQ, Sun J, Wang HX, Ng TB (2009) A novel lectin with antiproliferative activity from the medicinal mushroom Pholiota adiposa. Acta Biochim Pol 56: 415-421.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p299kz
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