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2010 | 57 | 3 | 265-268
Article title

Microbial transformation of citral by Penicillium sp.

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Thymol is present in the essential oils from herbs and spices, such as thyme. It is produced by these plant species as a chemical defense against phytopathogenic microorganisms. Therefore, this compound has attracted great attention in food industry, i.e., it has been used as a natural preservative in foods such as cheese to prevent fungal growth. Previous studies concerning the biotransformation of nerol by Penicillium sp. and microbial transformation of citral by sporulated surface cultures method (SSCM) of Penicillium digitatum have been reported. The objective of this research was to study the pathway involved during biotransformation of citral by Penicillium sp. using two methods. The culture preparation was done using different microbial methods and incubation periods to obtain Penicillium for citral biotransformation. The biotransformation products were identified by gas chromatography (GC) and gas chromatography/mass spectroscopy (GC/MS). A comparison of the two methods showed that SSCM was more effective, its major products were thymol (21.5%), geranial (18.6%) and nerol (13.7%). LM produced only one compound - thymol - with a low efficiency.
Physical description
  • Adams R (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured Publ. Corp., Carol Stream. IL,USA.
  • Chen FC, Chenm CF, Wei RD (1982) Acute toxicity of PR Toxin, a mycotoxin from Penicillium roqueforti. Toxicon 20: 433-441.
  • Demyttenaere JCR, Carme Herrera M, De Kimpe N (2000) Biotransformation of geraniol, nerol and citral by sporulated surface cultures of Aspergillus niger and Penicillium sp. Phytochemistry 55: 363-373.
  • Demyttenaere JCR, De Pooter HL (1998) Biotransformation of citral and nerol by spores of Penicillium digitatum. Flav Frag J 13: 1029-1036.
  • Esmaeili A, Sharafian S, Safaiyan S, Rezazadeh S, Rustaiyan A (2009a) Biotransformation of one monoterpene by sporulated surface cultures of Aspergillus niger and Penicillium sp. Nat Prod Res 23: 1058-1061.
  • Esmaeili A, Saad N, Safaiyan S, Rustaiyan A (2009b) Biotransformation of (-)-menthol by spores of Mucor ramannianus and study of the pathways involved. Herba Polonica 55: 51-59.
  • Esmaeili A, Hoseiny Zarea A, Sharafian S, Safaiyan S, Rustaiyan A (2009c) Biotransformation of menthol by sporulated surface cultures of Penicillium sp. and study of the pathways involved. Herba Polonica 55: 110-116.
  • Frank RK, Orth R, Ivankovic S, Kuhlmann M, Schmähl D (1977) Investigations on carcinogenic effects of Penicillium caseicolum and P. roqueforti in rats. Experientia 33: 515-516.
  • Goto K (1967) Biochemical degradation of geraniol by Aspergillus niger. Obihiro Chikusandaiagaku Gakujutsu Kenkyu Hokoku 4: 489-496; Chemical Abstracts 68;19904y,1968.
  • Ishihara K, Hamada H, Hirata T, Nakajima N (2003) Biotransformation using plant cultured cells. J Mol Catal B: Enzym 23: 145-170.
  • Janssens L, De Pooter HL, Schamp NM, Vandamme EJ (1992) Production of flavours by microorganism. Proc Biochem 27: 195-198.
  • Larroche C, Arpah M, Gros JB (1989) Methylketone by Caalginate/Eudrasit RL entrapped spores of Penicillium roqueforti. Enzyme Microb Technol 11: 106-112.
  • Larroche C, Gros JB (1989) Batch and continuous heptanone production Caalginate/Eudrasit RL entrapped spores of Penicillium roqueforti application to aroma production. Biotechnol Bioeng 34: 30-38.
  • Madyastha KM, Krishna Murthy NSR (1988a) Transformation of acetates of citronellol, geraniol and linalool by Aspergillus niger: regiospecific hydroxylation of citronellol by a cell-free system. Appl Microb Biotechnol 28: 324-329.
  • Madyastha KM, Krishna Murthy NSR (1988b) Regiospecific hydroxylation of acyclic monoterpene alcohols by Aspergillus niger. Tetrahedron Lett 29: 579-580.
  • Massada Y (1976) In Analysis of essential oil by gas chromatography and mass spectrometry. Wiley, New York.
  • Scharpf LG Jr, Seitz EW, Morris JA, Farbood MI (1986) Generation of flavour and odour compounds through fermentation processes. In Biogeneration of Aromas. Parliment TH, Croteau R, eds, 317, pp 323-346. American Chemical Socity, Washington, DC, ACS Symposium Series.
  • Shimoda K, Kondo Y, Nishida T, Hamada H, Nakajima N, Hamada H (2006) Biotransformation of thymol, carvacrol, and eugenol by cultured cells of Eucalyptus perriniana. Phytochemistry 67: 2256-2261.
  • Suga T, Hirata T (1990) Biotransformation of exogenous substrates by plant cell cultures. Phytochemistry 29: 2393-2406.
  • Ramaswami SK, Briscese P, Gargiullo R, Vonngeldern T (1988) In Flavours and Fragrances. A World Perspective. Lawrence BM, Mookerjee BD, Willis BJ, eds. Elsevier: Amsterdam 951.
  • Wood JB (1969) Microbial fermentation of lower terpenoids. Process Biochem 2: 50-52.
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