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2010 | 57 | 2 | 199-208
Article title

Interval hypoxic training in complex treatment of Helicobacter pylori-associated peptic ulcer disease

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This study was aimed to demonstrate the efficacy of interval hypoxic training (IHT) in complex treatment of Helicobacter pylori-associated duodenal peptic ulcer disease (DPUD) by parameters of aerobic metabolism and indexes of heart rate variability (HRV). Eighty patients with H. pylori-associated DPUD were included into the study, mean age 32±1.8 yrs, duration of the disease up to 10 years (66.3 %). IHT was modulated using Frolov's hypoxicator (TDI-01) for 30 days after standard eradication therapy. Daily hypoxic sessions consisted of three one-minute sessions, one two-minute, and one three-minute sessions separated by one-minute intervals of room-air breathing. Use of IHT resulted in more efficient elimination of clinical symptoms, histological hallmarks of inflammation and signs of oxidative stress in glandulocytes of the gastric mucosa as determined by 4-hydroxynonenal accumulation. Moderate prooxidant activity of IHT was demonstrated by the increased level of TBARS and oxidatively modified products, normalization of hydroperoxides, middle mass molecules and atherogenic beta-lipoproteins with simultaneous increase in catalase activity and mild decline of SOD activity. Therefore, IHT appeared to be accompanied by higher intensity of redox reactions and enhanced regeneratory processes in cells and tissues. Significant increase in HRV was also noted. Such changes were associated with reduction of inflammation signs and modulation of the autonomic homeostasis in DPUD patients. In general, use of IHT in complex treatment of H. pylori in DPUD patients can be recommended to increase resistance to oxidative stress and to modulate autonomic balance and oxidative homeostasis.
Physical description
  • Department of Internal Medicine No.1, Danylo Halytskyi National Medical University, Lviv, Ukraine
  • Department of Histology, Cytology Embryology, Danylo Halytskyi National Medical University, Lviv, Ukraine
  • Department of Organic, Biorganic and Pharmaceutical Chemistry, Danylo Halytskyi National Medical University, Lviv, Ukraine
  • Department of Internal Medicine No.1, Danylo Halytskyi National Medical University, Lviv, Ukraine
  • Division of Pathology, University Hospital Centre 'Zagreb', Zagreb, Croatia
  • Department of Histology, Cytology Embryology, Danylo Halytskyi National Medical University, Lviv, Ukraine
  • Division of Molecular Medicine, Institute 'Ruđjer Boškovič', Zagreb, Croatia
  • Division of Molecular Medicine, Institute 'Ruđjer Boškovič', Zagreb, Croatia
  • Division of Molecular Medicine, Institute 'Ruđjer Boškovič', Zagreb, Croatia
  • Arumugam TV, Gleichmann M, Tang SC, Mattson MP (2006) Hormesis/preconditioning mechanisms, the nervous system and aging. Ageing Res Rev 5: 165-178.
  • Augusto AC, Miguel F, Mendonca S, Pedrazzoli J Jr, Gurgueira SA (2007) Oxidative stress expression status associated to Helicobacter pylori virulence in gastric diseases. Clin Biochem 40: 615-622.
  • Bell EL, Emerling BM, Chandel NS (2005) Mitochondrial regulation of oxygen sensing. Mitochondrion 322-332.
  • Bernardi L, Passino C, Serebrovskaya Z, Serebrovskaya T, Appenzeller O (2001) Respiratory and cardiovascular adaptations to progressive hypoxia; effect of interval hypoxic training. Eur Heart J 22: 879-886.
  • Bunn HF, Poyton RO (1996) Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev 76: 839-885.
  • Cherkas A, Yelisyeyeva O, Semen K, Zarkovic K, Kaminskyy D, Gasparovic AC, Jaganjac M, Lutsyk A, Waeg G, Zarkovic N (2009) Persistent accumulation of 4-hydroxynonenal-protein adducts in gastric mucosa after Helicobacter pylori eradication. Coll Antropol 33: 815-821.
  • Connes P, Hue O, Hardy-Dessources MD, Boucher JH, Pichot V, Barthelemy JC (2008) Hemorheology and heart rate variability: is there a relationship? Clin Hemorheol Microcirc 38: 257-265.
  • Das DK, Maulik N (2003) Preconditioning potentiates redox signaling and converts death signal into survival signal. Arch Biochem Biophys 420: 305-311.
  • Das DK, Maulik N (2006) Cardiac genomic response following preconditioning stimulus. Cardiovasc Res 70: 254-263.
  • Denisov ET, Afanas'ev IB (2005) Oxidation and antioxidants in organic chemistry and biology. Taylor & Francis Group, Boca Raton.
  • Dubinina E, Burmistrov S, Khodov D, Porotov I (1995) Oxidative modification of human blood serum proteins: a method for its estimation. Vopr Med Khimii 41: 24-29 (in Russian).
  • Ellis EM (2007) Reactive carbonyls and oxidative stress: potential for therapeutic intervention. Pharmacol Ther 115: 13-24.
  • Everett SM, Singh R, Leuratti C, White KL, Neville P, Greenwood D, Marnett LJ, Schorah CJ, Forman D, Shuker D, Axon AT (2001) Levels of malondialdehyde-deoxyguanosine in the gastric mucosa: relationship with lipid peroxidation, ascorbic acid, and Helicobacter pylori. Cancer Epidemiol Biomarkers Prev 10: 369-376.
  • Giordano FJ (2005) Oxygen, oxidative stress, hypoxia, and heart failure. J Clin Invest 115: 500-508.
  • Gurusamy N, Lekli I, Gorbunov NV, Gherghiceanu M, Popescu LM, Das DK (2009) Cardioprotection by adaptation to ischaemia augments autophagy in association with BAG-1 protein. J Cell Mol Med 13: 373-387.
  • Guzy RD, Schumacker PT (2006) Oxygen sensing by mitochondria at complex III: The paradox of increased ROS during hypoxia. Exp Physiol 91: 807-819.
  • Haensel A, Mills PJ, Nelesen RA, Ziegler MG, Dimsdale JE (2008) The relationship between heart rate variability and inflammatory markers in cardiovaskular diseases. Psychoneuroendocrinology 33: 1305-1312.
  • Task Force of the European Society of Cardiology and the North American Society of Pacing and Electrophysiology (1996) Heart rate variability. Standards of measurement, physiological interpretation, and clinical use. Eur Heart J 17: 354-381.
  • Jones DP (2006) Disruption of mitochondrial redox circuitry in oxidative stress. Chem Biol Interact 163: 38-53.
  • Kamada T, Hata J, Kusunoki H, Ito M, Tanaka S, Kawamura Y, Chayama K, Haruma K (2005) Eradication of Helicobacter pylori increases the incidence of hyperlipidaemia and obesity in peptic ulcer patients. Dig Liver Dis 37: 39-43.
  • Kamyshnikov VS (ed) (2000) Reference on clinical biochemical laboratory diagnostics (in 2 vol). Minsk: Belarus: a) vol 1: 344-351; b) vol 2: 323-325; c) vol. 2: 334-342
  • Katayama K, Fujita H, Sato K, Ishida K, Iwasaki K-I, Miyamura M (2005) Effect of a repeated series of Intermittent hypoxic exposures on ventilatory response in humans. High Alt Med Biol 6: 50-59.
  • Kawada T, Mizuno M, Shimizu S, Uemura K, Kamiya A, Sugimachi M (2009) Angiotensin II disproportionally attenuates dynamic vagal and sympathetic heart rate controls. Am J Physiol Heart Circ Physiol 296: H1666-H1674.
  • Khomenko T, Deng X, Sandor Z, Tarnawski AS, Szabo S (2004) Cysteamine alters redox state, HIF-1a transcriptional interactions and reduces duodenal mucosal oxygenation: novel insight into the mechanisms of duodenal ulceration. Biochem Biophys Res Commun 317: 121-127.
  • Korolyuk M, Ivanova L, Mayorova I, Tokarev V (1998) A method for measuring catalase activity. Lab Delo 1 16-19 (in Russian).
  • Kostyuk V, Potapovich A, Kovaleva Z (1990) A simple sensitive assay for determination of superoxide dismutase activity based on reaction of quercetin oxidation. Vopr Med Khimii 88-91 (in Russian).
  • Levine BD (2002) Intermittent hypoxic training: fact and fancy. High Alt Med Biol 3: 177-193.
  • Malaty HM (2007) Epidemiology of Helicobacter pylori infection. Best Pract Res Clin Gastroenterol 21: 205-214.
  • Mangoni ME, Nargeot J (2008) Genesis and regulation of the heart automaticity. Physiol Rev 88: 919-982.
  • Manoli I, Alesci S, Blackman MR, Su YA, Rennert OM, Chrousos GP (2007) Mitochondria as key components of the stress response. Trends Endocrinol Metab 18: 190-198.
  • Marquez-Quinones A, Cipak A, Zarkovic K, Fateel-Fazenda S, VillaTrevino S, Waeg G, Zarkovic N, Gueraud F (2010) HNE-protein adducts formation in different pre-carcinogenic stages of hepatitis in LEC rats. Free Radic Res 44: 119--.
  • Matthews GM, Butler RN (2005) Cellular mucosal defense during Helicobacter pylori infection: a review of the role of glutathione and the oxidative pentose pathway. Helicobacter 10: 298-306.
  • Mattson MP (2008) Hormesis defined. Ageing Res Rev 7: 1-7.
  • Morton JP, Cable NT (2005) Effects of intermittent hypoxic training on aerobic and anaerobic performance. Ergonomics 48: 1535-1546.
  • Neubauer JA (2001) Physiological and genomic consequences of intermittent hypoxia. Invited review: physiological and pathophysiological responses to intermittent hypoxia. J Appl Physiol 90: 1593-1599.
  • Packer L, Cadenas E (2007) Oxidants and antioxidants revisited. New concepts of oxidative stress. Free Radic Res 41: 951-952.
  • Pongchaidecha A, Lailerd N, Boonprasert W, Chattipakorn N (2009) Effects of curcuminoid supplement on cardiac autonomic status in high-fat-induced obese rats. Nutrition 25: 870-878.
  • Prabhakar NR (2001) Oxygen sensing during intermittent hypoxia: cellular and molecular mechanisms. J Appl Physiol 90: 1986-1994.
  • Prabhakar NR, Peng YJ, Jacono FJ, Kumar GK, Dick TE (2005) Cardiovascular alterations by chronic intermittent hypoxia: importance of carotid body chemoreflexes. Clin Exp Pharmacol Physiol 32: 447-449.
  • Prabhakar NR, Kumar GK, Nanduri J, Semenza GL (2007) ROS signaling in systemic and cellular responses to chronic intermittent hypoxia. Antioxid Redox Signal 9: 1397-1403.
  • Suerbaum S, Michetti P (2002) Helicobacter pylori infection. N Engl J Med 347: 1175-1186.
  • Timirbulatov R, Seleznev E (1986) Methods for increasing intensity of free radical oxidation of blood lipids containing components and its diagnostic value. Lab Delo 4: 209-211 (in Russian).
  • Tymochko M, Kobylinska LI., Aleksevych YI. (1998) The role of free radical reactions in formation of the organism's oxygen homeostasis. Hypoxia Medical J 6: 154-158.
  • Uchida K (2003) 4-Hydroxynonenal: a product and mediator of oxidative stress. Prog Lipid Res 42: 318-343.
  • Voeikov VL (2005) Biological oxidation: over a century of hardship for the concept of active oxygen. Cell Mol Biol 51: 663-675.
  • von Kanel R, Thayer JF, Fischer JE (2009) Nighttime vagal cardiac control and plasma fibrinogen levels in a population of working men and women. Ann Noninvasive Electrocardiol 14: 176-184.
  • Waeg G, Dimisity G, Esterbauer H (1996) Monoclonal antibodies for detection of 4-hydroxynonenal modified proteins. Free Radic Res 25: 149-159.
  • Williams SE, Wootton P, Mason HS, Bould J, Iles DE, Riccardi D, Peers C, Kemp PJ (2004) Hemoxygenase-2 is an oxygen sensor for a calcium-sensitive potassium channel. Science 306: 2093-2097.
  • Yelisyeyeva O, Serhiyenko O, Cherkas A, Semen K, Kaminskyy D, Kurkevych A (2005) Heart rate variability in patients with diabetes mellitus: an attempt of metabolic interpretation. Probl Endocrine Pathol 95-110 (in Ukrainian).
  • Yelisyeyeva O, Cherkas A, Zarkovic K, Semen K, Kaminskyy D, Waeg G, Zarkovic N (2008) The distribution of 4-hydroxynonenal-modified proteins in gastric mucosa of duodenal peptic ulcer patients. Free Radic Res 42: 205-211.
  • Zarember KA, Malech HL (2005) HIF-1alpha: a master regulator of innate host defenses? J Clin Invest 115: 1702-1704.
  • Zarkovic N (2003) 4-Hydroxynonenal as a bioactive marker of pathophysiological processes. Mol Aspects Med 24: 281-291.
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