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2010 | 57 | 2 | 193-198
Article title

Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

Content
Title variants
Languages of publication
EN
Abstracts
EN
A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 µM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.
Keywords
Publisher

Year
Volume
57
Issue
2
Pages
193-198
Physical description
Dates
published
2010
received
2009-12-11
revised
2010-05-06
accepted
2010-06-03
(unknown)
2010-06-08
Contributors
author
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
author
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
author
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
author
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
author
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain
References
  • Ades EW, Candal FJ, Swerlick RA, George VG, Summers S, Bosse DC, Lawley TJ (1992) HMEC-1: establishment of an immortalized human microvascular endothelial cell line. J Invest Dermatol 99: 683-690.
  • Cully M, Downward J (2009) Translational responses to growth factors and stress. Biochem Soc Trans 37: 284-288.
  • Daugherty A, Dunn JL, Rateri DL, Heinecke JW (1994) Myeloperoxidase, a catalyst for lipoprotein oxidation, is expressed in human atherosclerotic lesions. J Clin Invest 94: 437-444.
  • Grassi D, Desideri G, Croce G, Tiberti S, Aggio A, Ferri C (2009) Flavonoids, vascular function and cardiovascular protection. Curr Pharm Des 15: 1072-1084.
  • Harrison D, Griendling KK, Landmesser U, Hornig B, Drexler H (2003) Role of oxidative stress in atherosclerosis. Am J Cardiol 91: 7A-11A.
  • Heinecke JW (2003) Oxidative stress: new approaches to diagnosis and prognosis in atherosclerosis. Am J Cardiol 91: 12A-16A.
  • Hu FB (2009) Diet and lifestyle influences on risk of coronary heart disease. Curr Atheroscler Rep 11: 257-263.
  • Kato Y, Nagao A, Terao J, Osawa T (2003) Inhibition of myeloperoxidase-catalyzed tyrosylation by phenolic antioxidants in vitro. Biosci Biotechnol Biochem 67: 1136-1139.
  • Lee JR, Kim JK, Lee SJ, Kim KP (2009) Role of protein tyrosine nitration in neurodegenerative diseases and atherosclerosis. Arch Pharm Res 32: 1109-1118.
  • Manach C, Williamson G, Morand C, Scalbert A, Reémeésy C (2005) Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr 81: 230S-242S.
  • Martindale JL, Holbrook NJ (2002) Cellular response to oxidative stress: Signaling for suicide and survival. J Cell Physiol 192: 1-15.
  • Mocatta TJ, Pilbrow AP, Cameron VA, Senthilmohan R, Frampton CM, Richards AM, Winterbourn CC (2007) Plasma concentrations of myeloperoxidase predict mortality after myocardial infarction. J Am Coll Cardiol 49: 1993-2000.
  • Papadopoulus NG, Dedoussis GVZ, Spanakos, Gritzapis AD, Bazevanis CN, Papamichail M (1994) An improved fluorescence assay for the determination of lymphocyte cytotoxicity using flow cytometry. J Immunol Methods 177: 101-111.
  • Pincemail J, Deby C, Thirion A, de Bruyn-Dister M, Goutier R (1988) Human myeloperoxidase activity is inhibited in vitro by quercetin. Comparison with three related compounds. Experientia 44: 450-453.
  • Rosso R, Vieira TO, Leal PC, Nunes RJ, Yunes RA, Creczynski-Pasa TB (2006) Relationship between the lipophilicity of gallic acid n-alquil esters' derivatives and both myeloperoxidase activity and HOCl scavenging. Bioorg Med Chem 14: 6409-6413.
  • Roy H, Bhardwaj S, Yla-Herttuala S (2009) Molecular genetics of atherosclerosis. Hum Genet 125: 467-491.
  • Schindhelm RK, Van Der Zwan LP, Teerlink T, Scheffer PG (2009) Myeloperoxidase: A useful biomarker for cardiovascular disease risk stratification? Clin Chem 55: 1462-1470.
  • Serrano J, Jové M, Boada J, Bellmunt MJ, Pamplona R, Portero-Otín M (2009) Dietary antioxidants interfere with Amplex Red-coupled-fluorescence assays. Biochem Biophys Res Commun 388: 443-449.
  • Shiba Y, Kinoshita T, Chuman H, Taketani Y, Eiji Takeda, Kato Y, Naito M, Kawabata K, Ishisaka A, Terao A, Kawai Y (2008) Flavonoids as substrates and inhibitors of myeloperoxidase: molecular actions of aglycone and metabolites. Chem Res Toxicol 21: 1600-1609.
  • Witting P, Pettersson K, Östlund-Lindqvist A, Westerlund C, Wågberg M, Stocker R (1999) Dissociation of atherogenesis from aortic accumulation of lipid hydro(pero)xides in watanabe heritable hyperlipidemic rabbits. J Clin Invest 104: 213-220.
  • Yokoyama M (2004) Oxidant stress and atherosclerosis. Curr Opin Pharmacol 4: 110-115.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p193kz
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