Double-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects

• Authors: José Serrano , Ana Cipak , Jordi Boada , Hugo Gonzalo , Daniel Cacabelos , Anna Cassanye , Reinald Pamplona , Neven Zarkovic , Manuel Portero-Otin
• Languages of publication: EN

Abstracts

EN

A previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 µM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.

Keywords

EN

gallic acid; peroxidase; pro-oxidant; antioxidant; HMEC-1

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2010
• Volume: 57
• Issue: 2
• Pages: 193-198

Dates

published

2010

received

2009-12-11

revised

2010-05-06

accepted

2010-06-03

(unknown)

2010-06-08

Contributors

author

José Serrano

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Ana Cipak

• Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia

author

Jordi Boada

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Hugo Gonzalo

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Daniel Cacabelos

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Anna Cassanye

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Reinald Pamplona

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

author

Neven Zarkovic

• Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia

author

Manuel Portero-Otin

• NUTREN Department of Experimental Medicine, University of Lleida-IRBLleida, Lleida, Spain

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