PL EN


Preferences help
enabled [disable] Abstract
Number of results
2010 | 57 | 2 | 185-191
Article title

Influence of 4-hydroxynonenal and spleen cells on primary hepatocyte culture and a novel liver-derived cell line resembling hepatocyte stem cells

Content
Title variants
Languages of publication
EN
Abstracts
EN
Liver is a unique mammalian organ with a great capacity of regeneration related to its function. After surgical resection or injury, hepatic cells, especially hepatocytes, can proliferate rapidly to repair the damage and to regenerate the structure without affecting the function of the liver. Loss of catalase activity during regeneration indicates that oxidative stress is present in the liver not only in pathological conditions but also as a 'physiological' factor during regeneration. As we have shown in our previous work, liver stem cell-like cells treated with 4-hydroxynonenal (HNE), a cytotoxic and growth regulating lipid peroxidation product, recover in the presence of spleen cells. In the current study we characterized this novel cell line as liver-derived progenitor/oval-like cells, (LDP/OCs), i.e. functional liver stem-like cells. We showed that LDP/OC were OV6 positive, with abundant glycogen content in the cytoplasm and expressed α-fetoprotein, albumin, biliverdin reductase and γ-glutamyl transferase. Also, we compared their growth in vitro with the growth of cultured primary hepatocytes stressed with HNE and co-cultured with autologous spleen cells. The influence of spleen cells on HNE-treated primary hepatocytes and on LDP/OCs showed that spleen cells support in a similar manner the recovery of both types of liver cells indicating their important role in regeneration. Hence, LDP/OC cells may provide a valuable tool to study cell interactions and the role on HNE in liver regeneration.
Publisher

Year
Volume
57
Issue
2
Pages
185-191
Physical description
Dates
published
2010
received
2009-11-13
revised
2010-05-16
accepted
2010-05-29
(unknown)
2010-05-31
Contributors
author
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Cell Biology, University of Salzburg, Salzburg, Austria
author
  • Department of Surgery, University Hospital Sestre milosrdnice, Zagreb, Croatia
author
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Pathology, Medical Faculty, Clinical Hospital Centre Zagreb, Zagreb, Croatia
  • Department of Pathology, Medical Faculty, Clinical Hospital Centre Zagreb, Zagreb, Croatia
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Pathology, Medical Faculty, Clinical Hospital Centre Zagreb, Zagreb, Croatia
author
  • Department of Cell Biology, University of Salzburg, Salzburg, Austria
  • Division of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
References
  • Alison M, Sarraf C (1998) Hepatic stem cells. J Hepatol 29: 676-682.
  • Alison M, Golding M, Lalaniel N, Sarraf C (1998) Wound healing in the liver with particular reference to stem cells. Philos Trans R Soc Lond B Biol Sci 353: 877-894.
  • Alison MR, Poulsom R, Forbes SJ (2001) Update on hepatic stem cells. Liver 21: 367-373.
  • Alison MR, Vig P, Russo F, Bigger BW, Amofah E, Themis M, Forbes S (2004) Hepatic stem cells: from inside and outside the liver? Cell Prolif 37: 1-21.
  • Anderson K, Yin L, Macdonald C, Grant MH (1996) Immortalized hepatocytes as in vitro model systems for toxicity testing: the comparative toxicity of menadione in immortalized cells, primary cultures of hepatocytes and HTC hepatoma cells. Toxicol In Vitro 10: 721-727.
  • D'Archivio M, Scazzocchio B, Filesi C, Varì R, Maggiorella MT, Sernicola L, Santangelo C, Giovannini C, Masella R (2008) Oxidised LDL up-regulate CD36 expression by the Nrf2 pathway in 3T3-L1 preadipocytes. FEBS Lett 582: 2291-2298.
  • Awasthi YC, Sharma R, Cheng JZ, Yang Y, Sharma A, Singhal SS, Awasthi S (2003) Role of 4-hydroxynonenal in stress-mediated apoptosis signaling. Mol Asp Med 24: 219-230.
  • Bauer M, Winning J, Kortgen A (2005) Liver failure. Curr Opin Anaesthesiol 18: 111-116.
  • Borovic S, Tirzitis G, Tirzite D, Cipak A, Khoschsorur GA, Waeg G, Tatzber F, Scukanec-Spoljar M, Zarkovic N (2006) Bioactive 1,4-dihydroisonicotinic acid derivatives prevent oxidative damage of liver cells. Eur J Pharmacol 537: 12-19.
  • Bruzzonea P, Strom SC (2006) Historical aspects of hepatocyte transplantation. Transplant Proc 38: 1179-1180.
  • Chen BP, Berman JJ, Ching WM, Rice JM (1983) DNA breakage by methyl methanesulfonate and its repair in brain and liver term cells cultured from fetal rat and mouse. Chem Biol Interact 44: 63-77.
  • Cipak A, Borovic S, Scukanec-Spoljar M, Kirac I, Zarkovic N (2005) Possible involvement of 4-hydroxynonenal in splenocyte regulated liver regeneration. Biofactors 24: 217-226.
  • Dollé L, Best J, Mei J, Al Battah F, Reynaert H, van Grunsven LA, Greets A (2010) The quest for liver progenitor cells a practical point of view. J Hepatol 52: 117-129.
  • Duret C, Gerbal-Chaloin S, Ramos J, Fabre JM, Jacquet E, Navarro F, Blanc P, Sa-Cunha A, Maurel P, Daujat-Chavanieu M (2007) Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver. Stem Cells 25: 1779-1790.
  • Eckl PM, Whitcomb WR, Michalopoulos G, Jirtle RL (1987) Effects of EGF and calcium on adult parenchymal hepatocyte proliferation. J Cell Physiol 132: 363-366.
  • Esterbauer H, Schaur RJ, Zollner H (1991) Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 11: 81-128.
  • Fougère-Deschatrette C, Imaizumi-Scherrer T, Strick-Marchand H, Morosan S, Charneau P, Kremsdorf D, Faust DM, Weiss MC (2006) Plasticity of hepatic cell differentiation: bipotential adult mouse liver clonal cell lines competent to differentiate in vitro and in vivo. Stem Cells 24: 2098-2109.
  • Gondeau C, Pichard-Garcia L, Maurel P (2009) Cellular models for the screening and development of anti-hepatitis C virus agents. Pharmacol Ther 124: 1-22.
  • Grasl-Kraupp B, Ruttkay-Nedecky B, Koudelka H, Bukowska K, Bursch W, Schulte-Hermann R (1995) In situ detection of fragmented DNA (TUNEL assay) fails to discriminate among apoptosis, necrosis and autolytic cell death: a cautionary note. Hepatology 21: 1465-1468.
  • Kreuzer T, Grube R, Zarkovic N, Schaur RJ (1998) 4-Hydroxynonenal modifies the effects of serum growth factors on the expression of the c-fos proto-oncogene and the proliferation of HeLa carcinoma cells. Free Radic Biol Med 25: 42-49.
  • Kulkarni JS, Khanna A (2006) Functional hepatocyte-like cells derived from mouse embryonic stem cells: a novel in vitro hepatotoxicity model for drug screening. Toxicol In Vitro 20: 1014-1022.
  • Leonarduzzi G, Robbesyn F, Poli G (2004) Signaling kinases modulated by 4-hydroxynonenal. Free Radic Biol Med 37: 1694-1702.
  • Michalopoulos GK, DeFrances MC (1997) Liver regeneration. Science 276: 60-66.
  • Michalopoulos G, Cianciulli D, Novotny AR, Kligerman AD, Strom SC, Jirtle RL (1982) Liver regeneration studies with rat hepatocytes in primary culture. Cancer Res 42: 4673-4682.
  • Oberhammer FA, Pavelka M, Sharma S, Tiefenbacher R, Purchio AF, Bursch W, Schulte-Hermann R (1992) Induction of apoptosis in cultured hepatocytes and in regressing liver by transforming growth factor β1. Proc Natl Acad Sci USA 89: 5408-5412.
  • Oertel M, Shafritz DA (2008) Stem cells, cell transplantation and liver repopulation. Biochim Biophys Acta 1782: 61-74.
  • Okiawa I, Novikoff PM (1995) Catalase negative peroxisomes: transient appearance in rat hepatocytes during liver regeneration after partial hepatectomy. Am J Path 146: 673-686.
  • Paku S, Schnur J, Nagy P, Thorgeirsson SS (2001) Origin and structural evolution of the early proliferating oval cells in rat liver. Am J Pathol 158: 1313-1323.
  • Poli G, Albano E, Dianzani MU (1987) The role of lipid peroxidation in liver damage. Chem Phys Lipids 45: 117-142.
  • Qin AL, Zhou XO, Zhang W, Yu H, Xie Q (2004) Characterization and enrichment of hepatic progenitor cells in adult rat liver. World J Gastroenterol 10: 1480-1486.
  • Robino G, Parola M, Marra F, Caligiuri A, De Franco RM, Zamara E, Bellomo G, Gentilini P, Pinzani M, Dianzani UM (2000) Interaction between 4-hydroxy-2,3-alkenals and the platelet-derived growth factor-β receptor: Reduced tyrosine phosphorylation and downstream signaling in hepatic stellate cells. J Biol Chem 275: 40561-40567.
  • Selden C, Hodgson H (2004) Cellular therapies for liver replacement. Transpl Immunol 12: 273-288.
  • Simm A, Halle JP, Adam G (1994) Proliferative and metabolic capacity of rat embryo fibroblasts immortalized by c-myc depends on cellular age at oncogenic transfection. Eur J Cell Biol 65: 121-131.
  • Spagnoli FM, Amicone L, Tripodi M, Weiss MC (1998) Identification of a bipotential precursor cell in hepatic cell lines derived from transgenic mice expressing cyto-Met in the liver. J Cell Biol 143: 1101-1112.
  • Stolz DB, Mars WM, Petersen BE, Kim TH, Michalopoulos GK (1999) Growth factor signal transduction immediately after two-thirds partial hepatectomy in rat. Cancer Res 59: 3954-3960.
  • Streetz KL, Luedde T, Manns MP, Trautwein C (2000) Interleukin 6 and liver regeneration. Gut 47: 309-312.
  • Suc I, Meilhac O, Lajoie-Mazenc I, Vandaele J, Jurgens G, Salvayre R, Nègre-Salvayre A (1998) Activation of EGF receptor by oxidized LDL . FASEB J 12: 665-671.
  • Taub R (2004) Liver regeneration: from myth to mechanism. Nat Rev Mol Cell Biol 5: 836-847.
  • Thorgeirsson SS, Grisham JW (2006) Hematopoietic cells as hepatocyte stem cells: a critical review of the evidence. Hepatology 43: 2-8.
  • Uchida K, Shiraishi M, Naito Y, Torii Y, Nakamura Y, Osawa T. (1999) Activation of stress signaling pathways by the end product of lipid peroxidation: 4-hydroxy-2-nonenal is a potential inducer of intracellular peroxide production. J Biol Chem 274: 2234-2242.
  • Zarkovic N (2003) 4-Hydroxynonenal as a bioactive marker of pathopysiological processes. Mol Aspects Med 24: 281-291.
  • Zarkovic N, Ilic Z, Jurin M, Scahur RJ, Puhl H, Esterbauer H (1993) Stimulation of HeLa cell growth by physiological con­centrations of 4-hydroxynonenal. Cell Biochem Funct 11: 279-286.
  • Zarkovic N, Zarkovic K, Schaur RJ, Stolc S, Schlag G, Redl H, Waeg G, Borovic S, Loncaric I, Juric G, Hlavka V (1999) 4-Hydroxynonenal as a second messenger of free radicals and growth modifying factor. Life Sci 65: 1901-1904.
  • Zindy F, Eischen CM, Randle DH, Kamijo T, Cleveland JL, Sherr CJ (1998) Myc signaling via the ARF tumor suppressor regulates p53-dependent apoptosis and immortalization. Genes Dev 12: 2424-2433.
  • Zivkovic M, Zarkovic K, Skrinjar LJ, Waeg G, Poljak-Blazi M, Borovic Sunjic S, Schaur RJ, Zarkovic N (2005) A new method for detection of HNE-histidine conjugates in rat inflammatory cells. Croat Chem Acta 78: 91-98.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p185kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.