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2010 | 57 | 2 | 179-183
Article title

Induction of CMV-1 promoter by 4-hydroxy-2-nonenal in human embryonic kidney cells

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Abstracts
EN
Oxidative stress, i.e., excessive production of oxygen free radicals and reactive oxygen species, leads to lipid peroxidation and to formation of reactive aldehydes which act as second messengers of free radicals. It has previously been shown that oxidative stress may be involved in the transcriptional regulation of cytomegalovirus (CMV) immediate early promoter, involved in viral reactivation from latency. In the current study we used a plasmid containing the yellow fluorescent protein (YFP) gene under the control of CMV-1 promoter to monitor the influence of hydrogen peroxide and reactive aldehydes, 4-hydroxy-2-nonenal (HNE) and acrolein, on CMV-1 promoter activation in human embryonic kidney cells (HEK293). While acrolein was ineffective, hydrogen peroxide slightly (50 %) stimulated the CMV promoter. In contrast, HNE had a strong, up to 3-fold, enhancing effect on the CMV-1 promoter within four as well as after 24h of treatment. The most effective was the treatment with 24 µM HNE. This effect of HNE suggests that stressful conditions associated with lipid peroxidation could lead to CMV activation.
Year
Volume
57
Issue
2
Pages
179-183
Physical description
Dates
published
2010
received
2009-11-10
revised
2010-04-22
accepted
2010-05-14
(unknown)
2010-05-31
Contributors
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
author
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
  • Biomedica Medizinprodukte GmbH & Co KG, Wien, Austria
  • Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia
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Publication order reference
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YADDA identifier
bwmeta1.element.bwnjournal-article-abpv57p179kz
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