Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)

• Authors: Jarosław Gzyl , Katarzyna Rymer , Edward Gwóźdź
• Languages of publication: EN

Abstracts

EN

Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.

Keywords

EN

cell suspension; cadmium; tolerance; superoxide dismutase; catalase; ascorbate peroxidase; guaiacol peroxidase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2009
• Volume: 56
• Issue: 4
• Pages: 723-727

Dates

published

2009

received

2009-06-15

revised

2009-10-05

accepted

2009-12-05

(unknown)

2009-12-10

Contributors

author

Jarosław Gzyl

• Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Katarzyna Rymer

• Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Edward Gwóźdź

• Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

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