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2009 | 56 | 4 | 723-727
Article title

Differential response of antioxidant enzymes to cadmium stress in tolerant and sensitive cell line of cucumber (Cucumis sativus L.)

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EN
Abstracts
EN
Previously, a stable cell suspension of cucumber tolerant to 100 µM CdCl2 was obtained (Gzyl & Gwóźdź, 2005, Plant Cell Tissue Organ Cult 80: 59-67). In this study, the relationship between the activity of antioxidant enzymes and cadmium tolerance of cucumber cells was analyzed. A cadmium-sensitive and the cadmium-tolerant cell lines were exposed to 100 µM and 200 µM CdCl2 and the activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APOX) and guaiacol peroxidase (POX) were determined. In the sensitive cell line, a decrease of total activity of SOD and POX was observed, whereas the activity of CAT and APOX significantly increased in metal-supplemented medium. By contrast, in the tolerant cells, the total activity of antioxidant enzymes decreased (SOD, CAT) or was maintained at approximately the same level (APOX, POX). Moreover, a different pattern of isoenzyme activity was observed in the tolerant and sensitive cells. These results suggest that an enhanced activity of antioxidant enzymes is not directly involved in the increased tolerance to cadmium of the selected cucumber cell line.
Publisher

Year
Volume
56
Issue
4
Pages
723-727
Physical description
Dates
published
2009
received
2009-06-15
revised
2009-10-05
accepted
2009-12-05
(unknown)
2009-12-10
Contributors
  • Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p723kz
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