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2009 | 56 | 4 | 711-715
Article title

Arachidonic acid-induced apoptosis in rat hepatoma AS-30D cells is mediated by reactive oxygen species

Content
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EN
Abstracts
EN
Arachidonic acid at micromolar concentrations produced a drastic increase of the generation of reactive oxygen species (ROS) in rat hepatoma AS-30D cells cultivated in vitro along with an increase in the incidence of apoptotic cell death. Both processes were prevented by trolox, a water-soluble tocopherol derivative, and tempol, a known antioxidative agent. A synthetic hybrid of lipoic acid and trolox or preincubation with N-acetylcysteine were less effective. Preincubation of the cells with etomoxir, a known highly specific irreversible inhibitor of carnitine-palmitoyltransferase I, partly decreased the ROS formation induced by arachidonic acid but it did not affect the increase in apoptosis. Cumulatively, these results indicate that apoptosis induced in hepatoma cells by arachidonic acid is mediated by ROS. They also suggest that this effect is due to arachidonic acid as such and not to its mitochondrial oxidative metabolites.
Publisher

Year
Volume
56
Issue
4
Pages
711-715
Physical description
Dates
published
2009
received
2009-08-27
revised
2009-11-09
accepted
2009-11-16
(unknown)
2009-12-01
Contributors
  • Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland
author
  • Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warszawa, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p711kz
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