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2009 | 56 | 1 | 63-69
Article title

Identification of the csp gene and molecular modelling of the CspA-like protein from Antarctic soil-dwelling psychrotrophic bacterium Psychrobacter sp. B6

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Abstracts
EN
We cloned and sequenced the cspA-like gene from a psychrotrophic Antarctic soil-dwelling bacterial strain Psychrobacter sp. B6. The gene is 213 bp long and shows 99% and 98% sequence identity with the Psychrobacter cryohalolentis K5 gene encoding a cold-shock DNA-binding domain protein and the Psychrobacter arcticus transcriptional regulator-CspA gene, respectively. The protein encoded by the Psychrobacter sp. B6 cspA-like gene shows 100% identity with the two proteins mentioned above, and also 61% sequence identity with CspB from Bacillus subtilis and Csp from Bacillus caldolyticus, and 56% - with Escherichia coli CspA protein. A three-dimensional model of the CspA-like protein from Psychrobacter sp. B6 was generated based on three known structures of cold shock proteins: the crystal structure of the major cold shock protein from Escherichia coli (CspA), the NMR structure of the latter protein, and the NMR structure of Csp from Thermotoga maritima. The deduced structure of the CspA-like protein from Psychrobacter sp. B6 was found to be very similar to these known structures of Csp-like proteins.
Publisher

Year
Volume
56
Issue
1
Pages
63-69
Physical description
Dates
published
2009
received
2008-08-04
revised
2009-01-12
accepted
2009-02-16
(unknown)
2009-02-24
Contributors
  • Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland
  • Centre of Medical Biology, Polish Academy of Sciences, Łódź, Poland
  • Centre of Medical Biology, Polish Academy of Sciences, Łódź, Poland
  • Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland
  • Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland
  • Institute of Technical Biochemistry, Technical University of Lodz, Łódź, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p63kz
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