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2009 | 56 | 4 | 635-640
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Glutathione reductase activity correlates with concentration of extracellular matrix degradation products in synovial fluid from patients with joint diseases

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The mechanisms underlying cartilage matrix degradation in joint diseases is not fully understood but reactive oxygen species are implicated as main causative factors. Comparative studies of glutathione reductase (GR) activity in synovial fluid from patients with rheumatoid arthritis (RA), reactive arthritis (ReA) and osteoarthritis (OA) as well as correlations between GR activity and concentration of the major cartilage components in synovial fluid are presented in this study. We found significantly higher activity of GR in RA (about three-fold) and ReA (about two-fold) than in OA. In RA and ReA patients, GR activity in synovial fluid correlates negatively with the concentrations of collagen and degradation products of sulfated glycosaminoglycans. In OA patients the activity of GR was significantly lower than in RA and ReA, which positively correlated with the concentration of collagen and showed a tendency for positive correlation with the degradation products of sulfated glycosaminoglycans. Our results suggest that in RA and ReA patients increased activity of GR does not prevent the increased degradation of collagen and proteoglycans by ROS.
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  • Department of Medical Chemistry, Medical University of Białystok, Białystok, Poland
  • Department of Medical Chemistry, Medical University of Białystok, Białystok, Poland
  • Department of Medical Chemistry, Medical University of Białystok, Białystok, Poland
  • Students' Scientific Group at the Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland
  • Department of Pediatrics and Nephrology, Medical University of Białystok, Białystok, Poland
  • Department of Pediatric Orthopaedics and Traumatology, Medical University of Białystok, Białystok, Poland
  • Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, Healey LA, Kaplan SR (1987) The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum 31: 315-324.
  • Bazzichi L, Ciompi ML, Betti L, Rossi A, Melchiorre D, Fiorini M, Giannaccini G, Lucacchini A (2002) Impaired glutathione reductase activity and levels of collagenase and elastase in synovial fluid in rheumatoid arthritis. Clin Exp Rheumatol 20: 761-766.
  • Biernat-Kałuża E (2001) Reactive arthritis as an interdisciplinary medical problem. Acta Clinica 1: 222-230.
  • Billinghurst RC, Dahlberg L, Ionescu M, Reiner A, Bourne R, Rorabeck C, Mitchell P, Hambor J, Diekmann O, Tischesche H, Chen J, Van Wart H, Poole AR (1997) Enhanced cleavage of type ll collagen by collagenases in osteoarthritic articular cartilage. J Clin Invest 99: 1534-1545.
  • Brandt KD, Fife RS, Braunstein EM, Katz B (1991) Radiological grading of the severity of knee osteoarthritis: relation of the Kellgren and Lawrence grade to a grade based on joint space narrowing, and correlation with arthroscopic evidence of articular cartilage degradation. Arthritis Rheum 34: 1381-1386.
  • Carlo MD, Loeser RF (2003) Increased oxidative stress with aging reduces chondrocyte survival: correlation with intracellular glutathione levels. Arthritis Rheum 48: 3419-3430.
  • Eguchi K (2007) Early prediction of joint destruction in rheumatoid arthritis. Clin Calcium 17: 517-525.
  • Farndale RW, Sayers CA, Barrett AJ (1982) A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res 9: 247-248.
  • Filippin LI, Vercelino R, Marroni NP, Xavier RM (2008) Redox signalling and the inflammatory response in rheumatoid arthritis. Clin Exp Immunol 152: 415-422.
  • Henrotin YE, Bruckner P, Pujol JP (2003) The role of reactive oxygen species in homeostasis and degradation of cartilage. Osteoarthritis Cartilage 11: 747-755.
  • Komsa-Penkova R, Spirova R, Bechev B (1996) Modification of Lowry's method for collagen concentration measurement. J Biochem Biophys Methods 32: 33-43.
  • Lark MW, Bayne EK, Flanagan J, Harper CF, Hoermer LA, Hutchinson NI, Singer II, Donatelli SA, Weidner JR, Williams HR, Mumford RA, Lohmander LS (1997) Aggrecan degradation in human cartilage. Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints. J Clin Invest 100: 93-106.
  • Monboisse JC, Borel JP (1992) Oxidative damage to collagen. EXS 62: 323-327
  • Olszowski S, Mak P, Olszowska E, Marcinkiewicz J (2003) Collagen type II modification by hypochlorite. Acta Biochim Polon 50: 471-479.
  • Ostalowska A, Birkner E, Wiecha M, Kasperczyk S, Kasperczyk A, Kopolka D, Zon-Giebel A (2006) Lipid peroxidation and antioxidant enzymes in synovial fluid of patients with primary and secondary osteoarthritis of the knee joint. Osteoarthritis Cartilage 14: 139-145.
  • Ostrowska J, Makieła M, Zwierz K (2004) Contribution of oxidative stress in liver injury. Hepatologia 4: 28-32.
  • Ortutay Z, Polgar A, Gomor B, Geher P, Lakatos T, Glant TT, Gay RE, Gay S, Pallinger E, Farkas C, Farkas E, Tothfalusi L, Kocsis K, Falus A, Buzas EI (2003) Synovial fluid exoglycosidases are predictors of rheumatoid arthritis and are effective in cartilage glycosaminoglycan depletion. Arthritis Rheum 48: 2163-2172.
  • Popko J, Marciniak J, Zalewska A, Górska A, Zwierz K, Sierakowski S, Urban M (2006a) Activity of N-acetyl-β-hexosaminidase and its isoenzymes in serum and synovial fluid from patients with different arthropathies. Clin Exp Rheumatol 24: 690-693.
  • Popko J, Marciniak J, Zalewska A, Małdyk P, Rogalski M, Zwierz K (2006b) The activity of exoglycosidases in the synovial membrane and knee fluid of patients with rheumatoid arthritis and juvenile idiopathic arthritis. Scand J Rheumatol 35: 189-192.
  • Rajagopalan S, Meng XP, Ramasamy S, Harrison DG, Galis ZS (1996) Reactive oxygen species produced by macrophage-derived foam cells regulate the activity of vascular matrix metalloproteinases in vitro: implications for atherosclerotic plaque stability. J Clin Invest 98: 2572-2579.
  • Rousseau IC, Delmas PD (2007) Biological markers in osteoarthritis. Nat Clin Pract Rheumatol 3: 346-356.
  • Shikman AR, Brinson DC, Lotz M (2000) Profile of glycosaminoglycan-degrading glycosidases and glycoside sulfatases secreted by human articular chondrocytes in homeostasis and inflammation. Arthritis Rheum 43: 1307-1314.
  • Sieper J, Braun J (1999) Problems and advances in the diagnosis of reactive arthritis. J Rheumatol 26: 1222-1234.
  • Taraza C, Mohora M, Vargolici B, Dinu V (1997) Importance of reactive oxygen species in rheumatoid arthritis. Rom J Intern Med 35: 89-98.
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