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2009 | 56 | 3 | 503-507
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Natural phenolic acids may increase serum estradiol level in ovariectomized rats

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Natural phenolic acids are commonly present in plants consumed in the diet. Recently we have observed that different natural phenolic acids exert differential effects on the body mass gain in ovariectomized and non-ovariectomized female rats. The aim of the present study was to investigate the effects of ferulic, caffeic, p-coumaric and chlorogenic acids on serum estradiol and total cholesterol levels in ovariectomized and non-ovariectomized rats. The experiments were carried out on 3-month old female Wistar Cmd:(WI)WU rats, divided into following groups (n=8 in each group): non-ovariectomized control rats and non-ovariectomized rats receiving ferulic, caffeic, p-coumaric or chlorogenic acids, sham-operated control rats, ovariectomized control rats and ovariectomized rats receiving the same phenolic acids. The phenolic acids were administered at a dose of 10 mg/kg p.o. daily for 4 weeks. Serum estradiol and total cholesterol levels on the next day after the last administration of the phenolic acids were examined. The phenolic acids did not affect serum estradiol or total cholesterol levels in non-ovariectomized rats. In ovariectomized rats, caffeic acid and to a lesser extent p-coumaric acid increased serum estradiol level, which effect correlated with a decreased body mass gain. All the phenolic acids decreased serum cholesterol level in ovariectomized rats. Concluding, the anti-obesity activity of some phenolic acids may be, at least partially, connected with estrogenic pathways.
Physical description
  • Department of Pharmacognosy and Phytochemistry, Medical University of Silesia, Katowice, Sosnowiec, Poland
  • Department of Pharmacology, Medical University of Silesia, Katowice, Sosnowiec, Poland
  • Department of Pharmacology, Medical University of Silesia, Katowice, Sosnowiec, Poland
  • Ardiansyah, Ohsaki Y, Shirakawa H, Koseki T, Komai M (2008) Novel effects of a single administration of ferulic acid on the regulation of blood pressure and the hepatic lipid metabolic profile in stroke-prone spontaneously hypertensive rats. J Agric Food Chem 56: 2825-2830.
  • Chang CJ, Chiu JH, Tseng LM, Chang CH, Chien TM, Wu CW, Lui WY (2006) Modulation of HER2 expression by ferulic acid on human breast cancer MCF7 cells. Eur J Clin Invest 36: 588-596.
  • Clegg DJ, Brown LM, Woods SC, Benoit SC (2006) Gonadal hormones determine sensitivity to central leptin and insulin. Diabetes 55: 978-987.
  • Folwarczna J, Zych M, Burczyk J, Trzeciak H, Trzeciak HI (2009) Effects of natural phenolic acids on the skeletal system of ovariectomized rats. Planta Med 75: 1-6.
  • Hany J, Lilienthal H, Sarasin A, Roth-Harer A, Fastabend A, Dunemann L, Lichtensteiger W, Winneke G (1999) Developmental exposure of rats to a reconstituted PCB mixture or aroclor 1254: effects on organ weights, aromatase activity, sex hormone levels, and sweet preference behavior. Toxicol Appl Pharmacol 158: 231-243.
  • Hsu C-L, Yen G-C (2008) Phenolic compounds: evidence for inhibitory effects against obesity and their underlying molecular signaling mechanisms. Mol Nutr Food Res 52: 53-61.
  • Innocenti G, Vegeto E, Dall'Acqua S, Ciana P, Giorgetti M, Agradi E, Sozzi A, Fico G, Tome F (2007) In vitro estrogenic activity of Achillea millefolium L. Phytomedicine 14: 147-152.
  • Jung EH, Kim SR, Hwang IK, Ha TY (2007) Hypoglycemic effects of a phenolic acid fraction of rice bran and ferulic acid in C57BL/KsJ-db/db mice. J Agric Food Chem 55: 9800-9804.
  • Kim HK, Jeong T-S, Lee M-K, Park YB, Choi M-S (2003) Lipid-lowering efficacy of hesperetin metabolites in high-cholesterol fed rats. Clin Chim Acta 327: 129-137.
  • Lafay S, Gueux E, Rayssiguier Y, Mazur A, Rémésy C, Scalbert A (2005) Caffeic acid inhibits oxidative stress and reduces hypercholesterolemia induced by iron overload in rats. Int J Vitam Nutr Res 75: 119-125.
  • Lee J-S, Bok S-H, Park YB, Lee M-K, Choi M-S (2003) 4-hydroxycinnamate lowers plasma and hepatic lipids without changing antioxidant enzyme activities. Ann Nutr Metab 47: 144-151.
  • Lee J-S, Choi M-S, Jeon S-M, Jeong T-S, Park YB, Lee M-K, Bok S-H (2001) Lipid-lowering and antioxidative activities of 3,4-di(OH)-cinnamate and 3,4-di(OH)-hydrocinnamate in cholesterol-fed rats. Clin Chim Acta 314: 221-229.
  • Liang Y-Q, Akishita M, Kim S, Ako J, Hashimoto M, Iijima K, Ohike Y, Watanabe T, Sudoh N, Toba K, Yoshizumi M, Ouchi Y (2002) Estrogen receptor β is involved in the anorectic action of estrogen. Int J Obes Relat Metab Disord 26: 1103-1109.
  • Meli R, Pacilio M, Raso GM, Esposito E, Coppola A, Nasti A, Di Carlo C, Nappi C, Di Carlo R (2004) Estrogen and raloxifene modulate leptin and its receptor in hypothalamus and adipose tissue from ovariectomized rats. Endocrinology 145: 3115-3121.
  • Pakrashi A, Pakrasi PL, Kabir SN (1979) Effect of p-coumaric acid on immature estrogen treated and cyclic female mice. Experientia 35: 843-844.
  • Sassa S, Kikuchi T, Shinoda H, Suzuki S, Kudo H, Sakamoto S (2003) Preventive effect of ferulic acid on bone loss in ovariectomized rats. In Vivo 17: 277-280.
  • Setchell KDR, Lydeking-Olsen E (2003) Dietary phytoestrogens and their effect on bone: evidence from in vitro and in vivo, human observational, and dietary intervention studies. Am J Clin Nutr 78 (Suppl 3): 593S-609S.
  • Sharma RD (1979) Isoflavones and hypercholesterolemia in rats. Lipids 14: 535-539.
  • Sri Balasubashini M, Rukkumani R, Menon VP (2003) Protective effects of ferulic acid on hyperlipidemic diabetic rats. Acta Diabetol 40: 118-122.
  • Stalikas CD (2007) Extraction, separation, and detection methods for phenolic acids and flavonoids. J Sep Sci 30: 3268-3295.
  • Stromeier S, Petereit F, Nahrstedt A (2005) Phenolic esters from the rhizomes of Cimicifuga racemosa do not cause proliferation effects in MCF-7 cells. Planta Med 71: 495-500.
  • Yamaguchi M, Lai YL, Uchiyama S, Nakagawa T (2008) Oral administration of phytocomponent p-hydroxycinnamic acid prevents bone loss in ovariectomized rats. Mol Cell Biochem 311: 31-36.
  • Zang L-Y, Cosma G, Gardner H, Shi X, Castranova V, Vallyathan V (2000) Effect of antioxidant protection by p-coumaric acid on low-density lipoprotein cholesterol oxidation. Am J Physiol Cell Physiol 279: C954-C960.
  • Zych M, Folwarczna J, Pytlik M, Śliwiński L, Gołden MA, Burczyk J, Trzeciak HI (2009) Administration of caffeic acid worsened bone mechanical properties in female rats (submitted).
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