Preferences help
enabled [disable] Abstract
Number of results
2009 | 56 | 3 | 465-469
Article title

Arginase isoenzymes in human cirrhotic liver

Title variants
Languages of publication
Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was performed on tissues obtained during liver transplantation from 60 patients with liver cirrhosis, and on samples of histologically normal liver (control) from 40 patients with benign or colorectal cancer liver metastases removed during surgery, 6-7 cm from the tumor border. Arginase isoenzymes AI (so-called liver-type arginase) and AII (called extrahepatic arginase) were identified by Western blotting and isolated by ion-exchange chromatography. Their expression on mRNA level was studied by RT-PCR. A significant decrease in arginase activity, dependent of the liver clinical stage, was observed in cirrhotic tissue. Arginase AI activity and its mRNA level were significantly decreased in cirrhotic liver, whereas the activity and expression of arginase AII were concurrently raised, as compared to normal liver. Since arginase AI is a key enzyme of the urea cycle, whereas arginase AII most probably takes part in the biosynthesis of ornithine and polyamines, the defective ammonia inactivation and increased collagen biosynthesis observed in cirrhotic liver may be related to the changes in arginase AI and AII levels, respectively.
Physical description
  • Aguilera V, Berenguer M, Rubin A, San-Juan F, Rayón JM, Prieto M, Mir J (2009) Cirrhosis of mixed etiology (hepatitis C virus and alcohol): Posttransplantation outcome - Comparison with hepatitis C virus-related cirrhosis and alcoholic-related cirrhosis. Liver Transpl 15: 79-87.
  • Brophy NA, Marie JP, Rojas VA, Warnke RA, McFall PJ, Smith SD, Sikic BI (1994) Mdr1 gene expression in childhood acute lymphoblastic leukemias and lymphomas: a critical evaluation by four techniques. Leukemia 8: 327-335.
  • Chinard FP (1952) Photometric estimation of proline and ornithine. J Biol Chem 199: 91-95.
  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
  • Chrzanowska A, Krawczyk M, Barańczyk-Kuźma A (2008) Changes in arginase isoenzymes pattern in human hepatocellular carcinoma. Biochem Biophys Res Commun 377: 337-340.
  • Cederbaum SD, Yu H, Grody WW, Kern RM, Yoo P, Iyer RK (2004) Arginases I and II: do their functions overlap? Mol Genet Metab 81: S38-S44.
  • Crombez EA, Cederbaum SD (2005) Hyperargininemia due to liver arginase deficiency. Mol Genet Metab 84: 243-2451.
  • Dioguardi N, Grizzi F, Fiamengo B, Russo C (2008) Metrically measuring liver biopsy: A chronic hepatitis B and C computer-aided morphologic description. World J Gastroenterol 14: 7335-7344.
  • Endo M, Oyadomari S, Terasaki Y, Takeya M, Suga M, Mori M, Gotoh T (2003) Induction of arginase I and II in bleomycin-induced fibrosis of mouse lung. Am J Physiol Lung Cell Mol Physiol 285: L313-L321.
  • Ferraz-Neto BH, Hidalgo R, Thomé T, Melo VA Jr, Lobue A, Zurstrassen MP, Moraes JM Jr, Meira-Filho SP, Rezende MB, Fonseca LE, Pandullo FL, Soeiro FS, Afonso RC (2007) Analysis of model for end-stage liver disease (MELD) score in a liver transplantation waiting list. Transplant Proc 39: 2511-2513.
  • Gebhardt R, Baldysiak-Figiel A, Krügel V, Ueberham E, Gaunitz F (2007) Hepatocellular expression of glutamine synthetase: an indicator of morphogen actions as master regulators of zonation in adult liver. Prog Histochem Cytochem 41: 201-266.
  • Grody WW, Kern RM, Klein D, Dodson AE, Wissman PB, Barsky SH, Cederbaum SD (1993) Arginase deficiency manifesting delayed clinical sequelae and induction of kidney arginase isoenzyme. Hum Genet 91: 1-5.
  • Häussinger D, Lamers WH, Moorman AF (1992) Hepatocyte heterogenity in the metabolism of amino acids and ammonia. Enzyme 46: 72-93.
  • Hillaire S, Voitot H (1999) Cirrhosis. Pathol Biol 47: 895-905.
  • Huo TI, Wu JC, Lin HC, Lee FY, Hou MC, Lee PC, Chang FY, Lee SD (2005) Evaluation of the increase in model for end-stage liver disease (DeltaMELD) score over time as a prognostic predictor in patients with advanced cirrhosis: risk factor analysis and comparison with initial MELD and Child-Turcotte-Pugh score. J Hepatol 42: 826-832.
  • Kato A, Watanabe Y, Sawara K, Suzuki K (2008) Diagnosis of sub-clinical hepatic encephalopathy by neuropsychological tests (NP-tests). Hepatol Res 38: S122-S127.
  • Kern RM, Grody WW, Cederbaum SD, Kern RM, Yoo P, Iyer RK Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
  • Nissim I, Luhovyy B, Horyn O (2005) The role of mitochondrially bound arginase in regulation of urea synthesis. J Biol Chem 280: 17715-17724.
  • Olga OZ, Nikolai DY (2003) Invasive and non-invasive monitoring of hepatitis C virus-induced liver fibrosis: alternatives or complements? Curr Pharm Biotechnol 4: 195-209.
  • O'sullivan D, Brosnan JT, Brosnan ME (1998) Hepatic zonation of the catabolism of arginine and ornithine in the perfused rat liver. Biochem J 330 (Part 2): 627-632.
  • Shah TU, Semelka RC, Pamuklar EZ, Firat Z, Gerber RD, Shrestha R, Russo MW (2006) The risk of hepatocellular carcinoma in cirrhotic patients with small liver nodules on MRI. Am J Gastroenterol 101: 533-540.
  • Sparkes RS, Dizikes GJ, Klisak I, Grody WW, Mohandas T, Heinzmann C, Zollman S, Lusis AJ, Cederbaum SD (1986) The gene for human liver arginase (ARG1) is assigned to chromosome band 6q23. Am J Hum Genet 39: 186-191.
  • Vockley JG, Jenkinson CP, Shukala H, Kern RM, Grody WW, Cederbaum SD (1996) Cloning and characterization of the human type II arginase gene. Genomics 38: 118-123.
  • Wheatley DN, Campbell E (2002) Arginine catabolism, liver extracts and cancer. Pathol Oncol Res 8: 18-25.
  • Yu J, Hui AY, Chu ES, Go MY, Cheung KF, Wu CW, Chan HL, Sung JJ (2009) The anti-inflammatory effect of celecoxib does not prevent liver fibrosis in bile duct-ligated rats. Liver Int 29: 25-36.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.