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2009 | 56 | 1 | 33-39
Article title

Giant unilamellar vesicles - a perfect tool to visualize phase separation and lipid rafts in model systems

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EN
Abstracts
EN
Model systems such as black lipid membranes or conventional uni- or multilamellar liposomes are commonly used to study membrane properties and structure. However, the construction and dimensions of these models excluded their direct optical microscopic observation. Since the introduction of the simple method of liposome electroformation in alternating electric field giant unilamellar vesicles (GUVs) have become an important model imitating biological membranes. Due to the average diameter of GUVs reaching up to 100 µm, they can be easily observed under a fluorescent or confocal microscope provided that the appropriate fluorescent probe was incorporated into the lipid phase during vesicle formation. GUVs can be formed from different lipid mixtures and they are stable in a wide range of physical conditions such as pH, pressure or temperature. This mini-review presents information about the methods of GUV production and their usage. Particularly, the use of GUVs in studying lipid phase separation and the appearance and behavior of lipid domains (rafts) in membranes is discussed but also other examples of GUVs use in membrane research are given. The experience of the authors in setting up the GUV-forming equipment and production of GUVs is also presented.
Publisher

Year
Volume
56
Issue
1
Pages
33-39
Physical description
Dates
published
2009
received
2009-01-19
revised
2009-03-10
accepted
2009-03-13
(unknown)
2009-03-17
Contributors
  • Department of Biophysics Wrocław Medical University, Wrocław, Poland
  • Department of Biophysics Wrocław Medical University, Wrocław, Poland
  • Department of Biophysics Wrocław Medical University, Wrocław, Poland
  • Department of Biology and Medical Parasitology, Wrocław Medical University, Wrocław, Poland
References
  • Akashi K, Miyata H, Itoh H, Kinosita K Jr (1996) Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope. Biophys J 71: 3242-3250.
  • Angelova MI, Dimitrov DS (1986) Liposome electroformation. Faraday Discuss Chem Soc 81: 303-311.
  • Angelova MI, Dimitrov DS (1988) A mechanism of liposome electroformation. Prog Colloid Polym Sci 76: 59-67.
  • Angelova MI, Tsoneva I (1999) Interactions of DNA with giant liposomes. Chem Phys Lipids 101: 123-137.
  • Angelova MI, Soleau S, Meleard Ph, Faucon JF, Bothorel F (1992) Preparation of giant vesicles by external AC electric fields. Kinetics and applications. Prog Colloid Polym Sci 89: 127-131.
  • Ayuyan AG, Cohen FS (2006) Lipid peroxides promote large rafts: effects of excitation of probes in fluorescence microscopy and electrochemical reactions during vesicle formation. Biophys J 91: 2172-2183.
  • Bacia K, Scherfeld D, Kahya N, Schwille P (2004) Fluorescence correlation spectroscopy relates rafts in model and native membranes. Biophys J 87: 1034-1043.
  • Bacia K, Schwille P, Kurzchalia T (2005) Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes. Proc Natl Acad Sci USA 102: 3272-3277.
  • Bagatolli LA, Gratton E (1999) Two-photon fluorescence microscopy observation of shape changes at the phase transition in phospholipid giant unilamellar vesicles. Biophys J 77: 2090-2101.
  • Bagatolli LA, Gratton E (2000a) Two photon fluorescence microscopy of coexisting lipid domains in giant unilamellar vesicles of binary phospholipid mixtures. Biophys J 78: 290-305.
  • Bagatolli LA, Gratton E (2000b) A correlation between lipid domain shape and binary phospholipid mixture composition in free standing bilayers: A two-photon fluorescence microscopy study. Biophys J 79: 434-447.
  • Baumgart T, Hunt G, Farkas ER, Webb WW, Feigenson GW (2007) Fluorescence probe partitioning between Lo/Ld phases in lipid membranes. Biochim Biophys Acta 1768: 2182-2194.
  • Dietrich C, Bagatolli LA, Volovyk ZN, Thompson NL, Levi M, Jacobson K, Gratton E (2001) Lipid rafts reconstituted in model membranes. Biophys J 80: 1417-1428.
  • Doeven M, Folgering JH, Krasnikov V, Geertsma ER, van den Bogaart G, Poolman B (2005) Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles. Biophys J 88: 1134-1142.
  • Evans E, Kwok R (1982) Mechanical calorimetry of large dimyristoylphosphatidylcholine vesicles in the phase transition region. Biochemistry 21: 4874-4879.
  • Fahsel S, Pospiech EM, Zein M, Hazlet TL, Gratton E, Winter R (2002) Modulation of concentration fluctuations in phase-separated lipid membranes by polypeptide insertion. Biophys J 83: 334-344.
  • Fischer A, Oberholzer T, Luisi PL (2000) Giant vesicles as models to study the interactions between membranes and proteins. Biochim Biophys Acta 1467: 177-188.
  • Gordon S, Berezhna S, Scherfeld D, Kahya N, Schwille P (2005) Characterization of interaction between cationic lipid-oligonucleotide complexes and cellular membrane lipids using confocal imaging and fluorescence correlation spectroscopy. Biophys J 88: 305-316.
  • Holopainen J, Angelova MI, Kinnunen PK (2000) Vectorial budding of vesicles by asymmetrical enzymatic formation of ceramide in giant liposomes. Biophys J 78: 830-838.
  • Kahya N, Scherfeld D, Bacia K, Poolman B, Schwille P (2003) Probing lipid mobility of raft-exhibiting model membranes by fluorescence correlation spectroscopy. J Biol Chem 278: 28109-28115.
  • Kahya N, Scherfeld D, Bacia K, Schwille P (2004) Lipid domain formation and dynamics in giant unilamellar vesicles explored by fluorescence correlation spectroscopy. J Struct Biol 147: 77-89.
  • Kenworthy AK (2005) Fleeting glimpses of lipid rafts: how biophysics is being used to track them. J Investig Med 53: 312-317.
  • Lee YH, Park NS, Kwon JD, Park JS, Shin GB, Lee CS, Jung TS, Choi NJ, Yoon JH, Ok JS, Yoon UC, Bae MK, Jang HO, Yun I (2007) Amphiphilic effects of dibucaine.HCl on rotational mobility of n-(9-anthroyloxy)stearic acid in neuronal and model membranes. Chem Phys Lipids 146: 33-42.
  • Lopez-Montero I, Rodriguez N, Cribier S, Pohl A, Velez M, Devaux PF (2005) Rapid transbilayer movement of ceramides in phospholipid vesicles and in human erythrocytes. J Biol Chem 280: 25811-25819.
  • Mathivet L, Cribier S, Devaux PF (1996) Shape change and physical properties of giant phospholipid vesicles prepared in the presence of an AC electric field. Biophys J 70: 1112-1121.
  • Metso AJ, Zhao H, Tuunainen I, Kinnunen PK (2005) Observation of the main phase transition of dinervonoylphosphocholine giant liposomes by fluorescence microscopy. Biochim Biophys Acta 1713: 83-91.
  • Needham D, Evans E (1988a) Structure and mechanical properties of giant lipid (DMPC) vesicle bilayers from 20 degrees C below to 10 degrees C above the liquid crystal-crystalline phase transition at 24 degrees C. Biochemistry 27: 8261-8269.
  • Needham D, McIntosh TJ, Evans E (1988b) Thermomechanical and transition properties of dimyristoylphosphatidylcholine/cholesterol bilayers. Biochemistry 27: 4668-4673.
  • Nomura SM, Mizutani Y, Kurita K, Watanabe A, Akiyoshi K (2005) Changes in the morphology of cell-size liposomes in the presence of cholesterol: formation of neuron-like tubes and liposome networks. Biochim Biophys Acta 1669: 164-169.
  • Riske KA, Dimova R (2005) Electro-deformation and poration of giant vesicles viewed with high temporal resolution. Biophys J 88: 1143-1155.
  • Scherfeld D, Kahya N, Schwille P (2003) Lipid dynamics and domain formation in model membranes composed of ternary mixtures of unsaturated and saturated phosphatidylcholines and cholesterol. Biophys J 85: 3758-3768.
  • Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387: 569-572.
  • Staneva G, Angelova MI, Koumanov K (2004) Phospholipase A2 promotes raft budding and fission from giant liposomes. Chem Phys Lipids 129: 53-62.
  • Staneva G, Seigneuret M, Koumanov K, Trugnan G, Angelova MI (2005) Detergents induce raft-like domains budding and fission from giant unilamellar heterogeneous vesicles. A direct microscopy observation. Chem Phys Lipids 136: 55-66.
  • Tamba Y, Tanaka T, Yahagi T, Yamashita Y, Yamazaki M (2004) Stability of giant unilamellar vesicles and large unilamellar vesicles of liquid-ordered phase membranes in the presence of Triton X-100. Biochim Biophys Acta 1667: 1-6.
  • Tierney KJ, Block DE, Longo ML (2005) Elasticity and phase behavior of DPPC membrane modulated by cholesterol, ergosterol, and ethanol. Biophys J 89: 2481-2493.
  • Veatch SL, Keller SL (2002) Organization in lipid membranes containing cholesterol. Phys Rev Lett 89: 268101-1-268101-4.
  • Veatch SL, Keller SL (2003) Separation of liquid phases in giant vesicles of ternary mixtures of phospholipids and cholesterol. Biophys J 85: 3074-3083.
  • Zhao J, Wu J, Heberle FA, Mills TT, Klawitter P, Huang G, Costanza G, Feigenson GW (2007) Phase studies of model biomembranes: complex behavior of DSPC/DOPC/cholesterol. Biochim Biophys Acta 1768: 2764-2776.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p33kz
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