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2009 | 56 | 2 | 325-335
Article title

Exogenous nitric oxide inhibits shedding of ADAM17 substrates

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EN
Abstracts
EN
Both ADAM17, the secretase responsible for the shedding of ectodomains of numerous membrane proteins including TNF and its receptors, as well as nitric oxide synthesized by inducible nitric oxide synthase play regulatory roles in inflammation and tumor progression. We analyzed the effect of endogenous and exogenous nitric oxide on the expression and activity of ADAM17 in murine endothelial cells and a monocyte/macrophage cell line. We found that endogenous nitric oxide influenced neither ADAM17 mRNA level nor the shedding of two ADAM17 substrates, TNF and TNFR1. Exogenous NO significantly diminished the release of TNF and TNFR1 without affecting the ADAM17 transcript level. Our data seem contrary to a previous report that showed the activation of ADAM17 by nitric oxide (Zhang et al., 2000, J Biol Chem 275: 15839-15844). We discuss potential mechanisms of NO-mediated inhibition of ectodomain shedding and possible reasons of discrepancy between our results and the previous report.
Keywords
Publisher

Year
Volume
56
Issue
2
Pages
325-335
Physical description
Dates
published
2009
received
2009-04-07
revised
2009-05-28
accepted
2009-06-17
(unknown)
2009-06-18
Contributors
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
author
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
author
  • Department of Cell Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland
References
  • Althoff K, Reddy P, Voltz N, Rose-John S, Mullberg J (2000) Shedding of interleukin-6 receptor and tumor necrosis factor α. Contribution of the stalk sequence to the cleavage pattern of transmembrane proteins. Eur J Biochem 267: 2624-2631.
  • Arribas J, Ruiz-Paz S (2005) ADAM17. Regulation of ectodomain shedding. In The ADAM family of proteases. Hooper NM, ed, pp 171-197. Springer, New York.
  • Bereta J, Bereta M (1995) Stimulation of glyceraldehyde-3-phosphate dehydrogenase mRNA levels by endogenous nitric oxide in cytokine-activated endothelium. Biochem Biophys Res Commun 217: 363-369.
  • Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP (1997) A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 385: 729-733.
  • Blanchot-Jossic F, Jarry A, Masson D, Bach-Ngohou K, Paineau J, Denis MG, Laboisse CL, Mosnier JF (2005) Up-regulated expression of ADAM17 in human colon carcinoma: co-expression with EGFR in neoplastic and endothelial cells. J Pathol 207: 156-163.
  • Bogdan C (2001) Nitric oxide and the immune response. Nat Immunol 2: 907-916.
  • Bohgaki T, Amasaki Y, Nishimura N, Bohgaki M, Yamashita Y, Nishio M, Sawada KI, Jodo S, Atsumi T, Koike T (2005) Up regulated expression of tumour necrosis factor {alpha} converting enzyme in peripheral monocytes of patients with early systemic sclerosis. Ann Rheum Dis 64: 1165-1173.
  • Borrell-Pages M, Rojo F, Albanell J, Baselga J, Arribas J (2003) TACE is required for the activation of the EGFR by TGF-α in tumors. Embo J 22: 1114-1124.
  • Bzowska M, Jura N, Lassak A, Black RA, Bereta J (2004) Tumour necrosis factor-alpha stimulates expression of TNF-α converting enzyme in endothelial cells. Eur J Biochem 271: 2808-2820.
  • Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
  • Colon AL, Menchen LA, Hurtado O, De Cristobal J, Lizasoain I, Leza JC, Lorenzo P, Moro MA (2001) Implication of TNF-α convertase (TACE/ADAM17) in inducible nitric oxide synthase expression and inflammation in an experimental model of colitis. Cytokine 16: 220-226.
  • Dejam A, Hunter CJ, Pelletier MM, Hsu LL, Machado RF, Shiva S, Power GG, Kelm M, Gladwin MT, Schechter AN (2005) Erythrocytes are the major intravascular storage sites of nitrite in human blood. Blood 106: 734-739.
  • Ding X, Yang LY, Huang GW, Wang W, Lu WQ (2004) ADAM17 mRNA expression and pathological features of hepatocellular carcinoma. World J Gastroenterol 10: 2735-2739.
  • Dunn BM (1995) Detection and assay methods. In: Current protocols in molecular biology. Ausubel F, Brent R, Kingston R, Moore D, Seidman J, Smith J, Struhl K, eds, pp 3.6.4-3.6.6. John Wiley & Sons, New York.
  • Eriksson S, Chambers BJ, Rhen M (2003) Nitric oxide produced by murine dendritic cells is cytotoxic for intracellular Salmonella enterica sv. Typhimurium. Scand J Immunol 58: 493-502.
  • Franovic A, Robert I, Smith K, Kurban G, Pause A, Gunaratnam L, Lee S (2006) Multiple acquired renal carcinoma tumor capabilities abolished upon silencing of ADAM17. Cancer Res 66: 8083-8090.
  • Garton KJ, Gough PJ, Blobel CP, Murphy G, Greaves DR, Dempsey PJ, Raines EW (2001) Tumor necrosis factor-α-converting enzyme (ADAM17) mediates the cleavage and shedding of fractalkine (CX3CL1). J Biol Chem 276: 37993-38001.
  • Garton KJ, Gough PJ, Philalay J, Wille PT, Blobel CP, Whitehead RH, Dempsey PJ, Raines W (2003) Stimulated shedding of vascular cell adhesion molecule 1 (VCAM-1) is mediated by tumor necrosis factor-α-converting enzyme (ADAM 17). J Biol Chem 278: 37459-37464.
  • Gomez-Jimenez J, Salgado A, Mourelle M, Martin MC, Segura MR, Peracaula R, Moncada S (1995) l-Arginine: nitric oxide pathway in endotoxemia and human septic shock. Crit Care Med 23: 253-258.
  • Gomez MI, Sokol SH, Muir AB, Soong G, Bastien J, Prince S (2005) Bacterial induction of TNF-α converting enzyme expression and IL-6 receptor α shedding regulates airway inflammatory signaling. J Immunol 175: 1930-1936.
  • Holst-Hansen C, Brünner N (1998) MTT-cell proliferation assay. In: Cell biology: A laboratory handbook. Celis JE, ed, pp 16-18. Academic Press, London.
  • Horiuchi K, Kimura T, Miyamoto T, Takaishi T, Okada Y, Toyama Y, Blobel CP (2007) Cutting edge: TNF-α-converting enzyme (TACE/ADAM17) inactivation in mouse myeloid cells prevents lethality from endotoxin shock. J Immunol 179: 2686-2689.
  • Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S (2005) Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-α release in patients with severe peritonitis: role of tumor necrosis factor-α converting enzyme cleavage. Crit Care Med 33: 1359-1364.
  • MacMicking JD, Nathan C, Hom G, Chartrain N, Fletcher DS, Trumbauer M, Stevens K, Xie QW, Sokol K, Hutchinson N (1995) Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase. Cell 81: 641-650.
  • Mężyk R, Bzowska M, Bereta J (2003) Structure and functions of tumor necrosis factor-α converting enzyme. Acta Biochim Polon 50: 625-645.
  • Mężyk-Kopeć R, Bzowska M, Potempa J, Bzowska M, Jura N, Sroka A, Black RA, Bereta J (2005) Inactivation of membrane tumor necrosis factor α by gingipains from Porphyromonas gingivalis. Infect Immun 73: 1506-1514.
  • Mężyk-Kopeć R, Bzowska M, Stalińska K, Chełmicki T, Podkalicki M, Jucha J, Kowalczyk K, Mak P, Bereta J (2009) Identification of ADAM10 as a major TNF sheddase in ADAM17-deficient fibroblasts. Cytokine 46: 309-315.
  • Milla ME, Gonzales PE, Leonard JD (2006) The TACE zymogen: re-examining the role of the cysteine switch. Cell Biochem Biophys 44: 342-348.
  • Moss ML, Jin SL, Milla ME, Bickett DM, Burkhart W, Carter HL, Chen WJ, Clay WC, Didsbury JR, Hassler D, Hoffman CR, Kost TA, Lambert MH, Leesnitzer MA, McCauley P, McGeehan G, Mitchell J, Moyer M, Pahel G, Rocque W, Overton LK, Schoenen F, Seaton T, Su JL, Becherer JD (1997) Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 385: 733-736.
  • Murray HW, Nathan CF (1999) Macrophage microbicidal mechanisms in vivo: reactive nitrogen versus oxygen intermediates in the killing of intracellular visceral Leishmania donovani. J Exp Med 189: 741-746.
  • Peschon JJ, Slack JL, Reddy P, Stocking KL, Sunnarborg SW, Lee DC, Russell WE, Castner BJ, Johnson RS, Fitzner JN, Boyce RW, Nelson N, Kozlosky CJ, Wolfson MF, Rauch CT, Cerretti DP, Paxton RJ, March CJ, Black RA (1998) An essential role for ectodomain shedding in mammalian development. Science 282: 1281-1284.
  • Reddy P, Slack JL, Davis R, Cerretti DP, Kozlosky CK, Blanton RA, Shows D, Peschon JJ, Black RA (2000) Functional analysis of the domain structure of tumor necrosis factor-alpha converting enzyme. J Biol Chem 275: 14608-14614.
  • Umansky V, Hehner SP, Dumont A, Hofmann TG, Schirrmacher V, Droge W, Schmitz ML (1998) Co-stimulatory effect of nitric oxide on endothelial NF-κB implies a physiological self-amplifying mechanism. Eur J Immunol 28: 2276-2282.
  • von Knethen A, Callsen D, Brune B (1999) NF-κB and AP-1 activation by nitric oxide attenuated apoptotic cell death in RAW 264.7 macrophages. Mol Biol Cell 10: 361-372.
  • Wang S, Zhang J, Zhang Y, Kern S, Danner RL (2008) Nitric oxide-p38 MAPK signaling stabilizes mRNA through AU-rich element-dependent and -independent mechanisms. J Leukoc Biol 83: 982-990.
  • Xu H, Uysal KT, Becherer JD, Arner P, Hotamisligil GS (2002) Altered tumor necrosis factor-α (TNF-α) processing in adipocytes and increased expression of transmembrane TNF-α in obesity. Diabetes 51: 1876-1883.
  • Zhang Z, Kolls JK, Oliver P, Good D, Schwarzenberger PO, Joshi MS, Ponthier JL, Lancaster R Jr (2000) Activation of tumor necrosis factor-α converting enzyme-mediate ectodomain shedding by nitric oxide. J Biol Chem 275: 15839-15844.
  • Zhang J, Wang S, Wesley RA, Danner RL (2003) Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites. J Biol Chem 278: 29192-29200.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p325kz
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