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2009 | 56 | 2 | 249-253
Article title

Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells

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EN
Abstracts
EN
Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.
Keywords
Publisher

Year
Volume
56
Issue
2
Pages
249-253
Physical description
Dates
published
2009
received
2008-12-19
revised
2009-04-15
accepted
2009-04-27
(unknown)
2009-05-07
Contributors
  • Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina
  • Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina
  • Instituto de la Visión; Hospital Oftalmológico Malvinas Argentinas; Buenos Aires, Argentina, Buenos Aires, Argentina
  • Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina
  • Department of Molecular Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland
  • Department of Molecular Biology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice, Poland
  • Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina
  • Laboratorio de Biomembranas, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Buenos Aires, Argentina
References
  • Asada H, Kishida T, Hirai H, Satoh E, Ohashi S, Takeuchi M, Kubo T, Kita M, Iwakura Y, Imanishi J, Mazda O (2002) Significant antitumor effects obtained by autologous tumor cell vaccine engineered to secrete interleukin 12 and IL-18 by means of the EBV/lipoplex. Mol Ther 5: 609-616.
  • Attia MA, Weiss DW (1966) Immunology of spontaneous mammary carcinomas in mice. V. Acquired tumor resistance and enhancement in strain-A mice infected with mammary tumor virus. Cancer Res 26: 1787-1800.
  • Bangham AD (1972) Model membranes. Chem Phys Lipids 8: 386-392.
  • Brunda MJ, Luistro L, Warrier RR, Wright RB, Hubbard BR, Murphy M, Wolf SF, Gately MK (1993) Antitumor and antimetastatic activity of interleukin 12 against murine tumors. J Exp Med 178: 1223-1230.
  • Budryk M, Wilczyńska U, Szary J, Szala S (2000) Direct transfer of IL-12 gene into growing Renca tumors. Acta Biochim Polon 47: 385-391.
  • Colombo MP, Trinchieri G (2002) Interleukin-12 in anti-tumor immunity and immunotherapy. Cytokine Growth Factor Rev 13: 155-168.
  • Elzaouk L, Moelling K, Pavlovic J (2006) Anti-tumor activity of mesenchymal stem cells producing IL-12 in a mouse melanoma model. Exp Dermatol 15: 865-874.
  • Fu J, Zheng J, Fang W (1998) Interleukin-12 gene transfection into murine B16 melanoma cells suppresses tumorigenicity and decreases metastatic potential. Zhonghua Yi Xue Za Zhi 78: 627-629.
  • Jacobsen F, Mertens-Rill J, Beller J, Hirsch T, Daigeler A, Langer S, Lehnhardt M, Steinau HU, Steinstraesser L (2006) Nucleofection: a new method for cutaneous gene transfer? J Biomed Biotechnol Article ID 26060, 2006: 1-8.
  • Kodama T, Takeda K, Shimozato O, Hayakawa Y, Atsuta M, Kobayashi K, Ito M, Yagita H, Okumura K (1999) Perforin-dependent NK cell cytotoxicity is sufficient for anti-metastatic effect of IL-12. Eur J Immunol 29: 1390-1396.
  • Lasek W, Basak G, Switaj T, Jakubowska AB, Wysocki PJ, Mackiewicz A, Drela N, Jalili A, Kamiński R, Kozar K, Jakóbisiak M (2004) Complete tumour regressions induced by vaccination with IL-12 gene-transduced tumour cells in combination with IL-15 in a melanoma model in mice. Cancer Immunol Immunother 53: 363-372.
  • Nanni P, Rossi I, De Giovanni C, Landuzzi L, Nicoletti G, Stoppacciaro A, Parenza M, Colombo MP, Lollini PL (1998) Interleukin 12 gene therapy of MHC-negative murine melanoma metastases. Cancer Res 58: 1225-1230.
  • Prehn R (2007) Immunostimulation and immunoinhibition of premalignant lesions. Theor Biol Med Model 4: 6.
  • Son K, Patel D, Tkach D, Park A (2000) Cationic liposome and plasmid DNA complexes formed in serum-free medium under optimum transfection condition are negatively charged. Biochim Biophys Acta 1466: 11-15.
  • Smyth MJ, Taniguchi M, Street SE (2000) The anti-tumor activity of IL-12: mechanisms of innate immunity that are model and dose dependent. J Immunol 165: 2665-2670.
  • Switaj T, Jalili A, Jakubowska AB, Drela N, Stoksik M, Nowis D, Basak G, Golab J, Wysocki PJ, Mackiewicz A, Sasor A, Socha K, Jakóbisiak M, Lasek W (2004) CpG immunostimulatory oligodeoxynucleotide 1826 enhances antitumor effect of interleukin 12 gene-modified tumor vaccine in a melanoma model in mice. Clin Cancer Res 10: 4165-4175.
  • Wang D, Jing N, Lin Q (1996) Stearylamine liposome as a new efficient reagent for dna transfection of eukaryotic cells. Biochem Biophys Res Commun 226: 450-455.
  • Wang JC, Liu XY, Lü WL, Lee HS, Goh BC, Zhang Q (2005) The in vitro cytotoxicity and in vivo toxicity of doxorubicin antiresistant stealth liposomes. Yao Xue Xue Bao 40: 475-480.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv56p249kz
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