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2008 | 55 | 1 | 97-106
Article title

Tenocyclidine treatment in soman-poisoned rats - intriguing results on genotoxicity versus protection

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Abstracts
EN
This study aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with ¼ and ½ of LD50 of soman. Possible genotoxic effects of TCP in white blood cells and brain tissue were also studied. Results were compared with previous findings on the adamantyl tenocyclidine derivative TAMORF. TCP given alone as pretreatment, 5 min before soman, seems to be superior in the protection of cholinesterase (ChE) catalytic activity in the plasma than in brain, especially after administration of the lower dose of soman. Plasma activities of the enzyme after a joint treatment with TCP and soman were significantly increased at 30 min (P < 0.001) and 24 h (P = 0.0043), as compared to soman alone. TCP and atropine, given as therapy, were more effective than TCP administered alone as a pretreatment. The above therapy significantly increased activities of the enzyme at 30 min (P = 0.046) and 24 h (P < 0.001), as compared to controls treated with ¼ LD50 of soman alone. Using the alkaline comet assay, acceptable genotoxicity of TCP was observed. However, the controversial role of TCP in brain protection of soman-poisoned rats should be studied further.
Publisher

Year
Volume
55
Issue
1
Pages
97-106
Physical description
Dates
published
2008
received
2007-11-24
revised
2008-02-04
accepted
2008-02-26
(unknown)
2008-03-07
Contributors
  • Forensic Centre "Ivan Vučetić", Ministry of Interior, Zagreb, Croatia
author
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
  • Institute for Medical Research and Occupational Health, Zagreb, Croatia
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p97kz
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