The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants

• Authors: Agnieszka Dzikiewicz-Krawczyk , Paulina Piontek , Zofia Szweykowska-Kulińska , Artur Jarmołowski
• Languages of publication: EN

Abstracts

EN

In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.

Keywords

EN

nuclear cap-binding protein complex; alternative splicing; mRNA surveillance; NMD; premature terminatin codon; nonsense-mediated mRNA decay

• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 4
• Pages: 825-828

Dates

published

2008

received

2008-06-26

revised

2008-09-18

accepted

2008-11-17

(unknown)

2008-12-16

Contributors

author

Agnieszka Dzikiewicz-Krawczyk

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Paulina Piontek

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Zofia Szweykowska-Kulińska

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Artur Jarmołowski

• Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

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