PL EN


Preferences help
enabled [disable] Abstract
Number of results
2008 | 55 | 4 | 825-828
Article title

The nuclear cap-binding protein complex is not essential for nonsense-mediated mRNA decay (NMD) in plants

Content
Title variants
Languages of publication
EN
Abstracts
EN
In this study we investigated whether in plants, like in mammals, components of the nuclear cap-binding protein complex (CBC) are involved in nonsense-mediated mRNA decay (NMD). We selected several genes producing at least two alternatively spliced mRNA variants: one with a premature termination codon (PTC+) and another without it (PTC-). For each gene the PTC+/PTC- ratio was calculated using RT-PCR and direct sequencing in four Arabidopsis thaliana lines: wild type, the NMD mutant atupf3-1 and two CBC mutants: cbp20 and abh1. Whereas in the NMD mutant the ratios of PTC+/PTC- splice variants were higher than in wild-type plants, the two CBC mutants investigated showed no change in the PTC+/PTC- ratios. Our results suggest that neither CBP20 nor CBP80 is involved in NMD in A. thaliana.
Publisher

Year
Volume
55
Issue
4
Pages
825-828
Physical description
Dates
published
2008
received
2008-06-26
revised
2008-09-18
accepted
2008-11-17
(unknown)
2008-12-16
Contributors
  • Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
References
  • Arciga-Reyes L, Wootton L, Kieffer M, Davies B (2006) Plant J 47: 480-489.
  • Chiu SY, Lejeune F, Ranganathan AC, Maquat LE (2004) Genes Dev 18: 745-754.
  • Conti E, Izaurralde E (2005) Curr Opin Cell Biol 17: 316-325.
  • Green RE (2003) Bioinformatics 19 (Suppl 1): i118-i121.
  • Hori K, Watanabe Y (2005) Plant J 43: 530-540.
  • Hosoda N, Kim YK, Lejeune F, Maquat LE (2005) Nat Struct Mol Biol 12: 893-901.
  • Hugouvieux V, Kwak JM, Schroeder JI (2001) Cell 106: 477-487.
  • Ishigaki Y, Li X, Serin G, Maquat LE (2001) Cell 106: 607-617.
  • Isshiki M, Yamamoto Y, Satoh H, Shimamoto K (2001) Plant Physiol 125: 1388-1395.
  • Kertesz S, Kerenyi Z, Merai Z, Bartos I, Palfy T, Barta E, Silhavy D (2006) Nucleic Acids Res 34: 6147-6157.
  • Kmieciak M, Simpson CG, Lewandowska D, Brown JWS, Jarmolowski A (2002) Gene 283: 171-183.
  • Lejeune F, Maquat LE (2005) Curr Opin Cell Biol 17: 309-315.
  • Maquat LE (2004) Nat Rev Mol Cell Biol 5: 89-99.
  • Papp I, Mur LA, Dalmadi A, Dulai S, Koncz C (2004) Plant Mol Biol 55: 679-686.
  • Petracek ME, Nuygen T, Thompson WF, Dickey LF (2000) Plant J 21: 563-569.
  • van Hoof A, Green PJ (1996) Plant J 10: 415-424.
  • Wagner E, Lykke-Andersen J (2002) J Cell Sci 115: 3033-3038.
  • Wang BB, Brendel V (2006) Proc Natl Acad Sci USA 103: 7175-7180.
  • Zhang S, Ruiz-Echevarria MJ, Quan Y, Peltz SW (1995) Mol Cell Biol 15: 2231-2244.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p825kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.