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2008 | 55 | 4 | 815-819
Article title

SNP-minisequencing as an excellent tool for analysing degraded DNA recovered from archival tissues

Content
Title variants
Languages of publication
EN
Abstracts
EN
SNP-minisequencing has become common in forensic genetics, especially for analysing degraded or low copy number DNA (LCN DNA). The aim of this study was to examine the usefulness of five SNP (single nucleotide polymorphism) markers for analyzing degraded and LCN DNA recovered from archival samples. DNA extractions of eight formalin-fixed paraffin-embedded (FFPE) tissues were performed and DNA fragments were amplified in one multiplex PCR (polymerase chain reaction). SNPs were identified in a minisequencing reaction and a gel electrophoresis in ABI Prism® 377 Sequencer. The research confirmed the usefulness of SNP-minisequencing for analysing FFPE tissues.
Publisher

Year
Volume
55
Issue
4
Pages
815-819
Physical description
Dates
published
2008
received
2008-04-27
revised
2008-07-08
accepted
2008-10-08
(unknown)
2008-11-4
Contributors
  • Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland
  • Department of Forensic Medicine, Medical University of Lodz, Łódź, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p815kz
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