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2008 | 55 | 4 | 799-805
Article title

Aldolase A is present in smooth muscle cell nuclei

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EN
Abstracts
EN
Previously we have shown that aldolase (ALD; EC 4.1.2.13) is present in cardiomyocyte nuclei. Now, we focused our attention on ALD localization in smooth muscle cells. Immunocytochemical methods were used to study the subcellular localization of ALD. Aldolase was localized in the cytoplasm as well as in the nuclei. Within the nuclei ALD was located in the heterochromatin region. Native polyacrylamide gel electrophoresis followed by aldolase activity staining in gel was used to study the ALD isoenzyme pattern in porcine smooth muscle cells. Two ALD isoenzymes, A and C, were found in these cells but in the nuclei only the muscle isoenzyme was detected. To support the nuclear localization of ALD, measurement of aldolase activity in the smooth muscle cell nuclei isolated from porcine stomach was performed. The ALD activity in the isolated nuclei was detectable only after preincubation of the nuclear fraction with Triton X-100 and high concentration of KCl.
Publisher

Year
Volume
55
Issue
4
Pages
799-805
Physical description
Dates
published
2008
received
2008-09-29
revised
2008-11-21
accepted
2008-12-08
(unknown)
2008-12-16
Contributors
author
  • Department of Animal Molecular Physiology, Institute of Zoology, University of Wrocław, Wrocław, Poland
  • Department of Genetics, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p799kz
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