PL EN


Preferences help
enabled [disable] Abstract
Number of results
2008 | 55 | 4 | 687-692
Article title

Genotype-specific human papillomavirus detection in cervical smears

Content
Title variants
Languages of publication
EN
Abstracts
EN
Human papillomavirus (HPV) is widely accepted as a causative agent of cervical cancer. The distribution and prevalence of HPV types depend on geographic region and demographic factors. The aim of this study was to investigate the relationship between the presence of various HPV types and the outcome of cytological examination. Cervical smears were obtained from 125 women from southern Poland: low grade squamous intraepithelial lesions (LSIL) - 44, high grade squamous intraepithelial lesions (HSIL) - 12, cervical carcinoma - 27 and 42 women without abnormality in cytology as a control group. DNA was extracted from the smears and broad-spectrum HPV DNA amplification and genotyping was performed with the SPF 10 primer set and reverse hybridisation line probe assay (INNO-LiPA HPV Genotyping, Innogenetics). HPV DNA was detected in approximately 72% cases, more frequently in women with squamous intraepithelial lesions and cervical carcinoma than in the control group (P < 0.0005). The most frequent type found was HPV 16 (37%), followed by HPV 51 (28%) and HPV 52 (17%). A single HPV type was detected in 51% positive cases, more frequently in cervical cancer specimens. Multiple HPV infection was dominant in women with LSIL and normal cytology. Prevalence of HPV 16 increased with the severity of cervical smear abnormality. For women HPV 16 positive, the relative risk (odds ratio) of the occurrence of HSIL and cervical cancer versus LSIL was 14.4 (95% CI, 3.0-69.2; P=0.001) and 49.4 (95% CI, 6.5-372.8; P < 0.001), respectively. Genotyping of HPV will allow better classification of women with cervical abnormalities into different risk groups and could be useful in therapy.
Publisher

Year
Volume
55
Issue
4
Pages
687-692
Physical description
Dates
published
2008
received
2008-04-27
revised
2008-09-16
accepted
2008-11-03
(unknown)
2008-11-17
Contributors
author
  • Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland
  • Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Kraków, Poland
  • Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland
  • Jagiellonian University Medical College, Chair of Microbiology, Department of Virology, Kraków, Poland
References
  • Bardin A, Vaccarella S, Clifford GM, Lissowska J, Rekosz M, Bobkiewicz P, Kupryjańczyk J, Krynicki R, Jonska-Gmyrek J, Danska-Bidzinska A, Snijders PJ, Meijer CJ, Zatonski W, Franceschi S (2008) Human papillomavirus infection in women with and without cervical cancer in Warsaw, Poland. Eur J Cancer 44: 557-564.
  • Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV (2002) The causal relation between human papillomavirus and cervical cancer. J Clin Pathol 55: 244-265.
  • Cho NH, An HJ, Jeong JK, Kang S, Kim YT, Park TK (2003) Genotyping of 22 human papillomavirus types by DNA chip in Korean women: Comparison with cytologic diagnosis. Am J Obstet Gynecol 188: 56-62.
  • Clavel C, Masure M, Bory JP, Putaud I, Mangeonjean C, Lorenzato M, Gabriel R, Quereux C, Birembaut P (1999) Hybrid Capture II-based human papillomavirus detection, a sensitive test to detect in routine high-grade cervical lesions: a preliminary study on 1518 women. Br J Cancer 80: 1306-1311.
  • Clifford GM, Smith JS, Aguado T, Franceschi S (2003a) Comparison of HPV type distribution in high-grade cervical lesions and cervical cancer: a meta-analysis. Br J Cancer 89: 101-105.
  • Clifford GM, Smith JS, Plummer M, Muñoz N, Franceschi S (2003b) Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysis. Br J Cancer 88: 63-73.
  • Clifford GM, Rana RK, Franceschi S, Smith JS, Gough G, Pimenta JM (2005) Human papillomavirus genotype distribution in low grade cervical lesions: comparison by geographic region and with cervical cancer. Cancer Epidemiol Biomarkers Prev 14: 1157-1164.
  • Cuzick J, Szarewski A, Terry G, Ho L, Hanby A, Moddox P, Anderson M, Kocjan G, Steele ST, Guillebaud J (1995) Human papillomavirus testing in primary cervical screening. Lancet 345: 1533-1536.
  • De Francesco MA, Gargiulo F, Schreiber C, Ciravolo G, Salinaro F, Manca N (2005) Detection and genotyping of human papillomavirus in cervical samples from Italian patients. J Med Virol 75: 588-592.
  • Dudkiewicz J, Waksmanski B, Cieslak-Stec M (2001) Detection of human papillomavirus genome in cervical squamous intraepitelial lesions. Ginekol Pol 72: 1489-1496 (in Polish).
  • Dybikowska A, Licznerski P, Podhajska A (2002) HPV detection in cervical cancer patients in northern Poland. Oncol Rep 9: 871-874.
  • Finan RR, Irani-Hakime N, Tamim H, Sharide HE, Daccache JL, Almawi WY (2001) Detection of human papillomavirus (HPV) genotypes in cervico-vaginal scrapes of women with normal and abnormal cytology. Clin Microbiol Infect 7: 688-692.
  • Frazer IH (2004) Prevention of cervical cancer through papillomavirus vaccination. Nat Rev Immunol 4: 46-54.
  • Gravitt PE, Peyton CL, Alessi TQ, Wheeler CM, Coutlee F, Hildesheim A, Schiffman MH, Scott DR, Apple RJ (2000) Improved amplification of genital human papillomaviruses. J Clin Microbiol 38: 357-361.
  • Guzik K, Bzowska M, Dobrucki J, Pryjma J (1999) Heat-shocked monocytes are resistant to Staphylococcus auterus-induced apoptotic DNA fragmentation due expression of HSP 72. Infect Immun 67: 4216-4222.
  • Harper DM, Franco EL, Wheeler C (2004) Efficacy of a bivalent L1 virus-like particle vaccine in prevention of infection with human papillomavirus types 16 and 18 in young women: a randomized controlled trial. Lancet 364: 1757-1765.
  • Jacobs MV, Snijders PJ, van der Brule AJ, Helmerhorst TJ, Meijer CJ, Walboomers JM (1997) A general primer GP5+/GP6+-mediated PCR enzyme immunoassay method for rapid detection of 14 high-risk and 6 low-risk human papillomavirus genotypes in cervical scrapings. J Clin Microbiol 35: 791-795.
  • Kleter B, van Doorn L-J, ter Schegget J, Schrauwen L, van Krimpen C, Burger M, ter Harmsel B, Quint W (1998) Novel short-fragment PCR assay for highly sensitive broad-spectrum detection of anogenital human papillomaviruses. Am J Pathol 153: 1731-1739.
  • Kleter B, van Doorn LJ, Schrauwen L, Molijn A, Sastrowijoto S, ter Schegget J, Linderman J, ter Harmsel B, Burger M, Quint W (1999) Development and clinical evaluation of a highly sensitive PCR-reverse hybridization line probe assay for detection and identification of anogenital human papillomavirus. J Clin Microbiol 37: 2508-2517.
  • Koutsky L, Ault KA, Wheeler CM (2002) A controlled trial of a human papillomavirus type 16 vaccine. N Engl J Med 347: 1645-1651.
  • Liss J, Lukaszuk K, Gulczynski J, Zwalinski M, Wozniak I, Emerich J, Wojcikowski C (2002) The incidence of human papillomavirus DNA in patients with cervical carcinoma from Gdansk region. Ginekol Pol 73: 740-744 (in Polish).
  • Low SH, Thong TW, Ho TH, Lee YS, Morita T, Singh M, Yap EH, Chan YC (1990) Prevalence of human papillomavirus types 16 and 18 in cervical carcinomas; a study by dot and Southern blot hybridization and the polymerase chain reaction. Jpn J Cancer Res 81: 1118-1123.
  • Manos MM, Ting Y, Wright DK, Lewis AI, Broker TR, Wolinsky SM (1989) The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells 7: 209-214.
  • Melchers WJG, Bakkers JMJE, Wang J, deWilde PCM, Boonstra H, Quint WGV, Hanselaar AGJM (1999) Short fragment polymerase chain reaction reverse hybridization line probe assay to detect and genotype a broad spectrum of human papillomavirus types. Am J Pathol 155: 1473-1478.
  • Meyer T, Arndt R, Beckmann ER, Padberg B, Christophers E, Stockfleth E (2001) Distribution of HPV 53, HPV 73 and CP8304 in genital epithelial lesions with different grades of dysplasia. Int J Gynecol Cancer 11: 198-204.
  • Michalski B, Mazurek U, Orchel J, Kachel-Flis A, Zielinski T, Łukasik A, Belowska A, Michalski M, Wilczok T (2004) Znaczenie badań wykrywajacych DNA-HPV w profilaktyce raka szyjki macicy. Wiad Lek 57 (Suppl 1): 201-206 (in Polish).
  • Muňoz N, Bosch FX, de Sanjose S, Herrero R, Castellsague X, Shah KV, Snijders PJF, Meijer CJLM (2003) Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med 348: 518-527.
  • Naucler P, Da Costa FM, Ljungberg O, Bugalho A, Dillner J (2004) Human papillomavirus genotypes in cervical cancer in Mozambique. J Gen Virol 85: 2189-2190.
  • Nyari TA, Kalmar L, Deak J, Szollosi J, Farkas I, Kovacs L (2004) Prevalence and risk factors of human papilloma virus infection in asymptomatic women in southeastern Hungary. Eur J Obstet Gynecol Reprod Biol 115: 99-100.
  • Perrons C, Kleter B, Jelley R, Jalal H, Quint W, Tedder RJ (2002) Detection and genotyping of human papillomavirus DNA by SPF 10 and MY09/11 primers in cervical cells taken from women attending a colposcopy clinic. J Med Virol 67: 246-252.
  • Quint WGV, Scholte G, van Doorn LJ, Kleter B, Smits PHM, Linderman J (2001) Comparative analysis of human papillomavirus infections in cervical scrapes and biopsy specimens by general SPF10 PCR and HPV genotyping. J Pathol 194: 51-58.
  • Remmink AJ, Walboomers JMM, Helmerhorts TJM, Voorhort FJ, Roozendaal L, Risse EKJ, Meijer CJLM, Kenemans P (1995) The presence of persistent high-risk HPV genotypes in dysplastic cervical lesions is associated with progressive disease natural history up to 36 months. Int J Cancer 61: 306-311.
  • Ronco G, Ghisetti V, Segnan N, Snijders PJ, Gillio-Tos A, Meijer CJ, Franceschi S (2005) Prevalence of human papillomavirus infection on women in Turin, Italia. Eur J Cancer 41: 297-305.
  • Schmidt MT, Olejnik AK, Gożdzicka-Józefiak A (2005) The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome. Acta Biochim Polon 52: 823-832.
  • Silins I, Wang X, Tadesse A, Jansen KU, Schiller JT, Avall-Lundqvist E, Frankendal B, Dillner J (2004) A population-based study of cervical carcinoma and HPV infection in Lativa. Gynecol Oncol 93: 484-492.
  • Szostek S, Klimek M, Zawilińska B, Ryś J, Kopeć J, Daszkiewicz E (2006) Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers. Acta Biochim Polon 53: 603-607.
  • Tachezy R, Salakova M, Hamšikova E, Kaňka J, Havrankova A, Vonka V (2003) Prospective study on cervical neoplasia: presence of HPV DNA in cytological smears precedes the development of cervical neoplastic lesions. Sex Transm Infect 79: 191-196.
  • Wojciechowska U, Didkowska J, Tarkowski W, Zatoński W (2005) Cancer in Poland in 2003. Center of Oncology, M. Sklodowska-Curie Memorial Institute, Warszawa (in Polish).
  • zur Hausen H (2000) Papillomaviruses causing cancer: evasion from host-cell control in early events in carcinogenesis. J Natl Cancer Inst 92: 690-698.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p687kz
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.