Methylenetetrahydrofolate reductase (MTHFR-677 and MTHFR-1298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis

• Authors: Igor Spiroski , Sashko Kedev , Slobodan Antov , Todor Arsov , Marija Krstevska , Sloboda Dzhekova-Stojkova , Gordana Bosilkova , Stojanka Kostovska , Dejan Trajkov , Aleksandar Petlichkovski , Ana Strezova , Olivija Efinska-Mladenovska , Mirko Spiroski
• Languages of publication: EN

Abstracts

EN

The aim was to investigate different genotypes and haplotypes of methylenetetrahydrofolate reductase (MTHFR-677, -1298) and plasma concentration of total homocysteine (tHcy) in Macedonian patients with occlusive artery disease (OAD) and deep venous thrombosis (DVT). Investigated groups consists of 80 healthy, 74 patients with OAD, and 63 patients with DVT. Plasma tHcy was measured with Microplate Enzyme Immunoassay. Identification of MTHFR genotypes and haplotypes was done with CVD StripAssay. The probability level (P-value) was evaluated by the Student's t-test. Plasma concentration of tHcy in CC and CT genotypes of MTHFR C677T was significantly increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy in AC genotype of MTHFR A1298C was increased in patients with OAD and in patients with DVT. Plasma concentration of tHcy was significantly increased in AA genotype of patients with OAD, but not in patients with DVT. We found a significant increase of plasma tHcy in patients with OAD in comparison with healthy respondents for normal:heterozygote (CC:AC), heterozygote:normal (CT:AA), and heterozygote:heterozygote (CT:AC) haplotypes. Plasma concentration of tHcy in patients with DVT in comparison with healthy respondents was significantly increased for normal:normal (CC:AA), normal heterozygote (CC:AC), and heterozygote:heterozygote (CT:AC) haplotypes. We conclude that MTHFR C677T and MTHFR A1289C genotypes and haplotypes are connected with tHcy plasma levels in Macedonian patients with OAD and DVT.

Keywords

EN

MTHFR-677; plasma total homocysteine; occlusive artery disease; MTHFR-1298; deep venous thrombosis

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 3
• Pages: 587-594

Dates

published

2008

received

2008-06-26

revised

2008-09-12

accepted

2008-09-16

(unknown)

2008-09-18

Contributors

author

Igor Spiroski

• Clinic for Cardiology, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Sashko Kedev

• Clinic for Cardiology, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Slobodan Antov

• Clinic for Cardiology, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Todor Arsov

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Marija Krstevska

• Institute of Medical and Experimental Biochemistry, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Sloboda Dzhekova-Stojkova

• Institute of Medical and Experimental Biochemistry, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Gordana Bosilkova

• Institute of Medical and Experimental Biochemistry, Faculty of Medicine, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Stojanka Kostovska

• Institute of Transfusion, Skopje, Republic of Macedonia

author

Dejan Trajkov

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Aleksandar Petlichkovski

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Ana Strezova

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Olivija Efinska-Mladenovska

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

author

Mirko Spiroski

• Institute of Immunobiology and Human Genetics, University "Ss. Kiril and Metodij", Skopje, Republic of Macedonia

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