Reactions of Pseudomonas aeruginosa pyocyanin with reduced glutathione

• Authors: Rajkumar Cheluvappa , Ronald Shimmon , Michael Dawson , Sarah N Hilmer , David G Le Couteur
• Languages of publication: EN

Abstracts

EN

Pseudomonas aeruginosa is the most common cause of chronic and recurrent lung infections in patients with cystic fibrosis (CF) whose sputa contain copious quantities of P. aeruginosa toxin, pyocyanin. Pyocyanin triggers tissue damage mainly by its redox cycling and induction of reactive oxygen species (ROS). The reactions between reduced glutathione (GSH) and pyocyanin were observed using absorption spectra from spectrophotometry and the reaction products analysed by nuclear magnetic resonance imaging. Pyocyanin reacted with GSH non-enzymatically at 37°C resulting in the production of red-brown products, spectophotometrically visible as a 480 nm maximum absorption peak after 24 h of incubation. The reaction was concentration-dependent on reduced glutathione but not on pyocyanin. Minimizing the accessibility of oxygen to the reaction decreased its rate. The anti-oxidant enzyme catalase circumvented the reaction. Proton-NMR analysis demonstrated the persistence of the original aromatic ring and the methyl-group of pyocyanin in the red-brown products. Anti-oxidant agents having thiol groups produced similar spectophotometrically visible peaks. The presence of a previously unidentified non-enzymatic GSH-dependent metabolic pathway for pyocyanin has thus been identified. The reaction between pyocyanin and GSH is concentration-, time-, and O2-dependent. The formation of H2O2 as an intermediate and the thiol group in GSH seem to be important in this reaction.

Keywords

EN

Pseudomonas aeruginosa pyocyanin; superoxide dismutase; catalase; spectrophotometry; nuclear magnetic resonance imaging; glutathione; cystic fibrosis; oxidative stress

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 3
• Pages: 571-580

Dates

published

2008

received

2008-05-24

revised

2008-08-26

accepted

2008-09-16

(unknown)

2008-09-17

Contributors

author

Rajkumar Cheluvappa

• Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney and Concord RG Hospital, Concord, Australia

author

Ronald Shimmon

• Department of Chemistry, Material and Forensic Science, University of Technology Sydney, Sydney, Australia

author

Michael Dawson

• Department of Chemistry, Material and Forensic Science, University of Technology Sydney, Sydney, Australia

author

Sarah N Hilmer

• Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney and Concord RG Hospital, Concord, Australia

author

David G Le Couteur

• Centre for Education and Research on Ageing and ANZAC Research Institute, University of Sydney and Concord RG Hospital, Concord, Australia

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