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2008 | 55 | 3 | 549-557
Article title

Effects of pH on the activity and structure of choline oxidase from Alcaligenes species

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A reversible effect of pH on the ionization of amino-acid residues at the active center of choline oxidase was observed near the optimum pH (8). Inactivation of choline oxidase took place in the pH ranges 3-6 and 9-11, in which irreversible changes in the structure occur leading to the enzyme inactivation. The first order rate constants of the enzyme's inactivation at various pH values were estimated for the irreversible changes. The Arrhenius analysis revealed no significant changes in the activation enthalpy, while an increase in the activation entropy reflected an increase in the conformational freedom.
Physical description
  • Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  • Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  • Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  • Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  • Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India
  • Ajloo D, Behnam H, Saboury AA, Mohamadi-Zonoz F, Ranjbar B, Moosavi-Movahedi AA, Hasani Z, Alizadeh K, Gharanfoli M, Amani M (2007) Thermodynamic and structural studies on the human serum albumin in the presence of a polyoxometalate. Bull Korean Chem Soc 28: 730-736.
  • Ali V, Prakash K, Kulkarni S, Ahmad A, Madhusudan KP, Bhakuni V (1999) 8-anilino-1-naphthalene sulphonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions. Biochemistry 38: 13635-13642.
  • Alia, Hayashi H, Sakamoto A, Murata N (1998) Enhancement of the tolerance of Arabidopsis to high temperatures by genetic engineering of the synthesis of glycinebetaine. Plant J 16: 155-161.
  • Bae JH, Anderson SH, Miller KJ (1993) Identification of a high-affinity glycine betaine transport system in Staphylococcus aureus. Appl Environ Microbiol 59: 2734-2736.
  • Bernheim F, Bernheim MLC (1933) Oxidation of acetylcholine by tissue. Am J Physiol 104: 438-440.
  • Bokvist M, Lindstrom F, Watts A, Grobner G (2004) Two types of Alzheimer's β-amyloid (1-40) peptide membrane interactions: aggregation preventing transmembrane anchoring versus accelerated surface fibril formation. J Mol Biol 335: 1039-1049.
  • Cardamone M, Puri NK (1992) Spectrofluorimetric assessment of the surface hydrophobicity of proteins. Biochem J 282: 589-593.
  • Culham DE, Emmerson KS, Lasby B, Mamelak D, Steer BA, Gyles CL, Villarejo M, Wood JM (1994) Genes encoding osmoregulatory proline/glycine betaine transporters and the proline catabolic enzymes are present and expressed in diverse clinical Escherichia coli isolates. Can J Microbiol 40: 397-402.
  • Deshnium P, Los DA, Hayashi H, Mustardy L, Murata N (1995) Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress. Plant Mol Biol 29: 897-907.
  • Deshnium P, Gombos Z, Nishiyama Y, Murata N (1997) The action in vivo of glycine betaine in enhancement of tolerance of Synechococcus sp. strain PCC 7942 to low temperature. J Bacteriol 179: 339-344.
  • Divsalar A, Saboury AA, Mansoori-Torshizi H, Hemmatinejad B (2006) Comparative and structural analysis of the interaction between β-lactoglobulin type A and B with a new anticancer compound (2,2'-bipyridin n-hexyl dithiocarbamato Pd(II) nitrate). Bull Korean Chem Soc 27: 1801-1808.
  • Dixon M, Webb EC (1979) Enzymes. 3rd ed, Chapter 4. Academic Press, New York.
  • Fan F, Gadda G (2007) An internal equilibrium preorganizes the enzyme-substrate complex for hydride tunneling in choline oxidase. Biochemistry 46: 6402-6408.
  • Gadda G (2003a) Kinetic mechanism of choline oxidase from Arthrobacter globiformis. Biochim Biophys Acta 1646: 112-118.
  • Gadda G (2003b) pH and deuterium kinetic isotope effects studies on the oxidation of choline to betaine-aldehyde catalyzed by choline oxidase Biochim Biophys Acta 1650: 4-9.
  • Gozdek A, Stankiewicz-Drogon A, Poznanski J, Boguszewska-Chachulska AM (2008) Circular dichroism analysis for multidomain proteins: studies of the irreversible unfolding of Hepatitis C virus helicase. Acta Biochim Polon 55: 57-66.
  • Graham JE, Wilkinson BJ (1992) Staphylococcus aureus osmoregulation: roles for choline, glycine betaine, proline, and taurine. J Bacteriol 174: 2711-2716.
  • Guerrieri A, Monaci L, Quinto M, Palmisano F (2002) A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts. Analyst 127: 5-7.
  • Holmstrom KO, Somersalo S, Mandal A, Palva TE, Welin B (2000) Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. J Exp Bot 51: 177-185.
  • Ikuta S, Imamura S, Mistake H, Horiuti Y (1977) Oxidative pathway of choline to betaine in the soluble fraction prepared from Arthrobacter globiformis. J Biochem 82: 157-163.
  • Kaenjak A, Graham JE, Wilkinson BJ (1993) Choline transport activity in Staphylococcus aureus induced by osmotic stress and low phosphate concentrations. J Bacteriol 175: 2400-2406.
  • Keesey J (1987) Boehringer Mannheim Biochemicals. 1st ed, p 58. IN Biochemica Information.
  • Kelly SM, Price NC (2000) The use of circular dichroism in the investigation of protein structure and fuction. Curr Protein Pept Sci 1: 349-384.
  • Kempf B, Bremer E (1998) Uptake and synthesis of compatible solutes as microbial stress responses to high-osmolality environments. Arch Microbiol 170: 319-330.
  • Khan SN, Islam B, Rajeswari MR, Usmani H, Khan AU (2008) interaction of anesthetic supplement thiopental with human serum albumin. Acta Biochim Polon 55: 399-409.
  • Kotik M, Zuber H (1993) Mutations that significantly change the stability, flexibility and quaternary structure of the l-lactate dehydrogenase from Bacillus megaterium. Eur J Biochem 211: 267-280.
  • Kuchel PW, Ralston GB (1988) Theory and problem of biochemistry. Chapter 9. Mc Graw Hill, New York.
  • Mann PJG, Woodward HE, Quastel JH (1938) Hepatic oxidation of choline and arsenoeholine. Biochem J 32: 1024-1032.
  • Michaelis L, Davidsohn H (1911) Die Wirkung der Wasserstoffionen auf das Invertin. Biochem Z 35: 386-412.
  • Nunes GS, Barcelo D (1998) Electrochemical biosensors for pesticide determination in food samples. Analysis Magazine 26: 156-159.
  • Ohta M, Miura R, Yamano T, Miyake Y (1983) Spectroscopic studies on the photoreaction of choline oxidase, a flavoprotein, with covalently bound flavin. J Biochem (Tokyo) 94: 879-892.
  • Ohta-Fukuyama M, Miyake Y, Emi S, Yamano T (1980) Identification and properties of the prosthetic group of choline oxidase from Alcaligenes sp. J Biochem 88: 197-203.
  • Okabe H, Sagesaka K, Nakjima N, Noma A (1977) New enzymatic. assay of cholinesterase activity. Clin Chim Acta 80: 87-94.
  • Peddie BA, Wong-She J, Randall K, Lever M, Chambers ST (1998) Osmoprotective properties and accumulation of betaine analogues by Staphylococcus aureus. FEMS Microbiol Lett 160: 25-30.
  • Quaye O, Lountos GL, Fan F, Orville AM, Gadda G (2008) Role of Glu312 in binding and positioning of the substrate for hydride transfer reaction in choline oxidase. Biochemistry 47: 243-256.
  • Saboury AA, Karbassi F, Haghbeen K, Ranjbar B, Moosavi-Movahedi AA, Farzami B (2004) Stability, structural and suicide inactivation changes of mushroom tyrosinase after acetylation by N-acetylimidazole. Int J Biol Macromol 34: 257-262.
  • Sakamoto A, Alia, Murata N (1998) Metabolic engineering of rice leading to biosynthesis of glycinebetaine and tolerance to salt and cold. Plant Mol Biol 38: 1011-1019.
  • Sakamoto S, Valverde R, Alia, Chen THH, Murata N (2000) Transformation with codA gene for choline oxidase enhances the freezing tolerance of Arabidopsis. Plant J 22: 449-454.
  • Sarraf NS, Saboury AA, Ranjbar B, Moosavi-Movahedi AA (2004) Structural and functional changes of bovine carbonic anhydrase as a consequence of temperature. Acta Biochim Polon 51: 665-671.
  • Schagger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379.
  • Sreerama N, Woody RW (2004) On the analysis of membrane protein circular dichroism spectra. Protein Sci 13: 100-112.
  • Tavakoli H, Ghourchian H, Moosavi-Movahedi AA, Chilaka FC (2005) Effects of paraoxon and ethylparathion on choline oxidase from Alcaligenes species: Inhibition and denaturation. Int J Biol Macromol 36: 318-323.
  • Williams IN, Litwack G (1952) Studies on rat liver choline oxidase: an assay method. J Biol Chem 192: 73-77.
  • Yamada H, Mori N, Tani Y (1979) Properties of choline oxidase of Cylindrocarpon didyum M-1. Agric Biol Chem 43: 2173-2177.
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