Pullulanase from rice endosperm

• Authors: Yoshiki Yamasaki , Susumu Nakashima , Haruyoshi Konno
• Languages of publication: EN

Abstracts

EN

Pullulanase (EC 3.2.1.41) in non-germinating seeds was compared with that in germinating seeds. Moreover, pullulanase from the endosperm of rice (Oryza sativa L., cv. Hinohikari) seeds was isolated and its properties investigated. The pI value of pullulanase from seeds after 8 days of germination was almost equal to that from non-germinating seeds, which shows that these two enzymes are the same protein. Therefore, the same pullulanase may play roles in both starch synthesis during ripening and starch degradation during germination in rice seeds. The enzyme was isolated by a procedure that included ammonium sulfate fractionation, DEAE-cellulofine column chromatography, preparative isoelectric focusing, and preparative disc gel electrophoresis. The enzyme was homogeneous by SDS/PAGE. The molecular weight of the enzyme was estimated to be 100 000 based on its mobility on SDS/PAGE and 105 000 based on gel filtration with TSKgel super SW 3000, which showed that it was composed of a single unit. The isoelectric point of the enzyme was 4.7. The enzyme was strongly inhibited by β-cyclodextrin. The enzyme was not activated by thiol reagents such as dithiothreitol, 2-mercaptoethanol or glutathione. The enzyme most preferably hydrolyzed pullulan and liberated only maltotriose. The pullulan hydrolysis was strongly inhibited by the substrate at a concentration higher than 0.1%. The degree of inhibition increased with an increase in the concentration of pullulan. However, the enzyme hydrolyzed amylopectin, soluble starch and β-limit dextrin more rapidly as their concentrations increased. The enzyme exhibited α-glucosyltransfer activity and produced an α-1,6-linked compound of two maltotriose molecules from pullulan.

Keywords

EN

glucosyl transfer; pullulanase; thiol reagent; endosperm; rice (Oryza sativa L. cv. Hinohikari)

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 3
• Pages: 507-510

Dates

published

2008

received

2008-04-21

revised

2008-09-04

accepted

2008-09-11

(unknown)

2008-09-12

Contributors

author

Yoshiki Yamasaki

• Research Institute for Bioresources, Okayama University, Kurashiki-shi, Okayama, Japan

author

Susumu Nakashima

• Research Institute for Bioresources, Okayama University, Kurashiki-shi, Okayama, Japan

author

Haruyoshi Konno

• Research Institute for Bioresources, Okayama University, Kurashiki-shi, Okayama, Japan

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