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2008 | 55 | 2 | 399-409
Article title

Interaction of anesthetic supplement thiopental with human serum albumin

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EN
Abstracts
EN
Thiopental (TPL) is a commonly used barbiturate anesthetic. Its binding with human serum albumin (HSA) was studied to explore the anesthetic-induced protein dysfunction. The basic binding interaction was studied by UV-absorption and fluorescence spectroscopy. An increase in the binding affinity (K) and in the number of binding sites (n) with the increasing albumin concentration was observed. The interaction was conformation-dependent and the highest for the F isomer of HSA, which implicates its slow elimination. The mode of binding was characterized using various thermodynamic parameters. Domain II of HSA was found to possess a high affinity binding site for TPL. The effect of micro-metal ions on the binding affinity was also investigated. The molecular distance, r, between donor (HSA) and acceptor (TPL) was estimated by fluorescence resonance energy transfer (FRET). Correlation between the stability of the TPL-N and TPL-F complexes and drug distribution is discussed. The structural changes in the protein investigated by circular dichroism (CD) and Fourier transform infrared (FT-IR) spectroscopy reflect perturbation of the albumin molecule and provide an explanation for the heterogeneity of action of this anesthetic.
Publisher

Year
Volume
55
Issue
2
Pages
399-409
Physical description
Dates
published
2008
received
2007-08-18
revised
2008-01-15
accepted
2008-01-31
(unknown)
2008-02-01
Contributors
author
  • Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India, Aligarh, India
author
  • Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India, Aligarh, India
author
  • Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India, Aligarh, India
author
  • Department of Anesthesiology, JN Medical College, AMU, Aligarh, India, Aligarh, India
author
  • Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India, Aligarh, India
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p399kz
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