PL EN


Preferences help
enabled [disable] Abstract
Number of results
2008 | 55 | 1 | 35-42
Article title

Differential binding of hyaluronan on the surface of tissue-specific endothelial cell lines

Content
Title variants
Languages of publication
EN
Abstracts
EN
Tissue-specific heterogeneity of endothelial cells, both structural and functional, plays a crucial role in physiologic as well as pathologic processes, including inflammation, autoimmune diseases and tumor metastasis. This heterogeneity primarily results from the differential expression of adhesion molecules that are involved in the interactions between endothelium and circulating immune cells or disseminating tumor cells. Among these molecules present on endothelial cells is hyaluronan (HA), a glycosaminoglycan that contributes to primary (rolling) interactions through binding to its main receptor CD44 expressed on leukocytes and tumor cells. While the regulation of CD44 expression and function on either leukocytes or tumor cells has been well characterized, much less is known about the ability of endothelial cells to express HA on their surface. Therefore, in these studies we analyzed HA levels on tissue-specific endothelium. We used endothelial cell lines of different origin, including lung, skin, gut and lymph nodes that had been established previously as model lines to study interactions between the endothelium and leukocytes/tumor cells. Our results indicate that HA is accumulated on the surface of all endothelial cells examined. Moreover, retention of endogenous HA differs between the lines and may depend on their tissue origin. Analysis of binding of exogenous HA reveals the presence of specific HA binding sites on all endothelial cell lines tested. However, the retention of endogenous HA and the binding of exogenous HA is mediated through a CD44-independent mechanism.
Keywords
Year
Volume
55
Issue
1
Pages
35-42
Physical description
Dates
published
2008
received
2007-10-23
revised
2007-12-03
accepted
2008-01-25
(unknown)
2008-01-30
References
  • Baldwin AL, Thurston G (2001) Mechanics of endothelial cell architecture and vascular permeability. Crit Rev Biomed Eng 29: 247-278.
  • Bielawska-Pohl A, Crola C, Caignard A, Gaudin C, Dus D, Kieda C, Chouaib S (2005) Human NK cells lyse organ-specific endothelial cells: analysis of adhesion and cytotoxic mechanisms. J Immunol 174: 5573-5582.
  • Bizouarne N, Denis V, Legrand A, Monsigny M, Kieda C (1993) A SV-40 immortalized murine endothelial cell line from peripheral lymph node high endothelium expresses a new alpha-l-fucose binding protein. Biol Cell 79: 209-218.
  • Brocke S, Piercy C, Steinman L, Weissman IL, Veromaa T (1999) Antibodies to CD44 and integrin alpha4, but not l-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment. Proc Natl Acad Sci USA 96: 6896-6901.
  • Butcher EC, Picker LJ (1996) Lymphocyte homing and homeostasis. Science 272: 60-66.
  • Butcher EC, Williams M, Youngman K, Rott L, Briskin M (1999) Lymphocyte trafficking and regional immunity. Adv Immunol 72: 209-253.
  • Camenisch TD, McDonald JA (2000) Hyaluronan: is bigger better? Am J Respir Cell Mol Biol 23: 431-433.
  • Camp RL, Scheynius A, Johansson C, Pure E (1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation. J Exp Med 178: 497-507.
  • Cichy J, Pure E (2000) Oncostatin M and transforming growth factor-beta 1 induce post-translational modification and hyaluronan binding to CD44 in lung-derived epithelial tumor cells. J Biol Chem 275: 18061-18069.
  • Cichy J, Pure E (2003) The liberation of CD44. J Cell Biol 161: 839-843.
  • Cichy J, Pure E (2004) Cytokines regulate the affinity of soluble CD44 for hyaluronan. FEBS Lett 556: 69-74.
  • Cichy J, Bals R, Potempa J, Mani A, Pure E (2002) Proteinase-mediated release of epithelial cell-associated CD44. Extracellular CD44 complexes with components of cellular matrices. J Biol Chem 277: 44440-44447.
  • Cichy J, Kulig P, Pure E (2005) Regulation of the release and function of tumor cell-derived soluble CD44. Biochim Biophys Acta 1745: 59-64.
  • Day AJ, Prestwich GD (2002) Hyaluronan-binding proteins: tying up the giant. J Biol Chem 277: 4585-4588.
  • de Belder AN, Wik KO (1975) Preparation and properties of fluorescein-labelled hyaluronate. Carbohydr Res 44: 251-257.
  • DeGrendele HC, Estess P, Siegelman MH (1997) Requirement for CD44 in activated T cell extravasation into an inflammatory site. Science 278: 672-675.
  • Estess P, DeGrendele HC, Pascual V, Siegelman MH (1998) Functional activation of lymphocyte CD44 in peripheral blood is a marker of autoimmune disease activity. J Clin Invest 102: 1173-1182.
  • Griffioen AW, Coenen MJ, Damen CA, Hellwig SM, van Weering DH, Vooys W, Blijham GH, Groenewegen G (1997) CD44 is involved in tumor angiogenesis; an activation antigen on human endothelial cells. Blood 90: 1150-1159.
  • Kieda C, Paprocka M, Krawczenko A, Zalecki P, Dupuis P, Monsigny M, Radzikowski C, Dus D (2002) New human microvascular endothelial cell lines with specific adhesion molecules phenotypes. Endothelium 9: 247-261.
  • Knudson W (1996) Tumor-associated hyaluronan. Providing an extracellular matrix that facilitates invasion. Am J Pathol 148: 1721-1726.
  • Knudson W, Aguiar DJ, Hua Q, Knudson CB (1996) CD44-anchored hyaluronan-rich pericellular matrices: an ultrastructural and biochemical analysis. Exp Cell Res 228: 216-228.
  • Knudson W, Chow G, Knudson CB (2002) CD44-mediated uptake and degradation of hyaluronan. Matrix Biol 21: 15-23.
  • Laurent TC, Fraser JR (1992) Hyaluronan. Faseb J 6: 2397-2404.
  • Lee JY, Spicer AP (2000) Hyaluronan: a multifunctional, megaDalton, stealth molecule. Curr Opin Cell Biol 12: 581-586.
  • Lokeshwar VB, Selzer MG (2000) Differences in hyaluronic acid-mediated functions and signaling in arterial, microvessel, and vein-derived human endothelial cells. J Biol Chem 275: 27641-27649.
  • Maiti A, Maki G, Johnson P (1998) TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation. Science 282: 941-943.
  • Mikecz K, Brennan FR, Kim JH, Glant TT (1995) Anti-CD44 treatment abrogates tissue oedema and leukocyte infiltration in murine arthritis. Nat Med 1: 558-563.
  • Mikecz K, Dennis K, Shi M, Kim JH (1999) Modulation of hyaluronan receptor (CD44) function in vivo in a murine model of rheumatoid arthritis. Arthritis Rheum 42: 659-668.
  • Mohamadzadeh M, DeGrendele H, Arizpe H, Estess P, Siegelman M (1998) Proinflammatory stimuli regulate endothelial hyaluronan expression and CD44/HA-dependent primary adhesion. J Clin Invest 101: 97-108.
  • Ponta H, Sherman L, Herrlich PA (2003) CD44: from adhesion molecules to signalling regulators. Nat Rev Mol Cell Biol 4: 33-45.
  • Siegelman MH, DeGrendele HC, Estess P (1999) Activation and interaction of CD44 and hyaluronan in immunological systems. J Leukoc Biol 66: 315-321.
  • Siegelman MH, Stanescu D, Estess P (2000) The CD44-initiated pathway of T-cell extravasation uses VLA-4 but not LFA-1 for firm adhesion. J Clin Invest 105: 683-691.
  • Tammi R, Paukkonen P, Wang C, Horsmanheimo M, Tammi M (1994) Hyaluronan and CD44 in psoriatic skin. Intense staining for hyaluronan on dermal capillary loops and reduced expression of CD44 and hyaluronan in keratinocyte-leukocyte interfaces. Arch Dermatol Res 286: 21-29.
  • Weiss L, Slavin S, Reich S, Cohen P, Shuster S, Stern R, Kaganovsky E, Okon E, Rubinstein AM, Naor D (2000) Induction of resistance to diabetes in non-obese diabetic mice by targeting CD44 with a specific monoclonal antibody. Proc Natl Acad Sci USA 97: 285-290.
  • Yu Q, Toole BP, Stamenkovic I (1997) Induction of apoptosis of metastatic mammary carcinoma cells in vivo by disruption of tumor cell surface CD44 function. J Exp Med 186: 1985-1996.
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p35kz
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.