Basic energetic parameters of Acanthamoeba castellanii mitochondria and their resistance to oxidative stress

• Authors: Wieslawa Jarmuszkiewicz , Nina Antos-Krzeminska , Danuta Drachal-Chrul , Karolina Matkovic , Wioletta Nobik , Joanna Pieńkowska , Aleksandra Swida , Andrzej Woyda-Ploszczyca , Malgorzata Budzinska
• Languages of publication: EN

Abstracts

EN

The purpose of this study was establishing the basic energetic parameters of amoeba Acanthamoeba castellanii mitochondria respiring with malate and their response to oxidative stress caused by hydrogen peroxide in the presence of Fe2+ ions. It appeared that, contrary to a previous report (Trocha LK, Stobienia O (2007) Acta Biochim Polon 54: 797), H2O2-treated mitochondria of A. castellanii did not display any substantial impairment. No marked changes in cytochrome pathway activity were found, as in the presence of an inhibitor of alternative oxidase no effects were observed on the rates of uncoupled and phosphorylating respiration and on coupling parameters. Only in the absence of the alternative oxidase inhibitor, non-phosphorylating respiration progressively decreased with increasing concentration of H2O2, while the coupling parameters (respiratory control ratio and ADP/O ratio) slightly improved, which may indicate some inactivation of the alternative oxidase. Moreover, our results show no change in membrane potential, Ca2+ uptake and accumulation ability, mitochondrial outer membrane integrity and cytochrome c release for 0.5-25 mM H2O2-treated versus control (H2O2-untreated) mitochondria. These results indicate that short (5 min) incubation of A. castellanii mitochondria with H2O2 in the presence of Fe2+ does not damage their basic energetics.

Keywords

EN

Acanthamoeba castellanii; mitochondria; hydrogen peroxide; oxidative stress

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 2
• Pages: 349-356

Dates

published

2008

received

2008-03-31

revised

2008-05-17

accepted

2008-05-30

(unknown)

2008-06-07

Contributors

author

Wieslawa Jarmuszkiewicz

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Nina Antos-Krzeminska

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Danuta Drachal-Chrul

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Karolina Matkovic

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Wioletta Nobik

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Joanna Pieńkowska

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Aleksandra Swida

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Andrzej Woyda-Ploszczyca

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

author

Malgorzata Budzinska

• Faculty of Biology, Adam Mickiewicz University, Poznań, Poland

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