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2008 | 55 | 1 | 201-206
Article title

Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase

Content
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EN
Abstracts
EN
His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.
Year
Volume
55
Issue
1
Pages
201-206
Physical description
Dates
published
2008
received
2007-10-23
revised
2008-01-29
accepted
2008-02-12
(unknown)
2008-03-07
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p201kz
Identifiers
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