Alteration of O-specific polysaccharide structure of symbiotically defective Mesorhizobium loti mutant 2213.1 derived from strain NZP2213

• Authors: Anna Turska-Szewczuk , Hubert Pietras , Wojciech Borucki , Ryszard Russa
• Languages of publication: EN

Abstracts

EN

Mesorhizobium loti mutant 2213.1 derived from the wild-type strain NZP2213 by Tn5 mutagenesis showed impaired effectiveness of symbiosis with the host plant Lotus corniculatus (Turska-Szewczuk et al., 2007 Microbiol Res, in press). The inability of lipopolysaccharide (LPS) isolated from the mutant 2213.1 strain or de-O-acetylated LPS of the parental cells to inactivate phage A1 particles implicated alterations in the LPS structure. The O-specific polysaccharide of the mutant was studied by chemical analyses along with 1H and 13C NMR spectroscopy, which clearly confirmed alterations in the O-chain structure. 2D NMR data showed that the mutant O-polysaccharide consists of a tetrasaccharide repeating unit containing non-substituted as well as O-acetylated or O-methylated 6-deoxytalopyranose residues. Additionally, an immunogold assay revealed a reduced number of gold particles on the mutant bacteroid cell surface, which could result from both a diminished amount of an O-antigenic determinant in mutant LPS and modifications of structural epitopes caused by alterations in O-acetylation or O-methylation of sugar residues. Western immunoblot assay of alkaline de-O-acetylated lipophilic M. loti NZP2213 LPS showed no reactivity with homologous serum indicating a role of O-acetyl groups in its O-specificity.

Keywords

EN

Mesorhizobium loti; symbiosis; 6-deoxytalose; O-specific polysaccharide; bacterial polysaccharide structure

• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 1
• Pages: 191-200

Dates

published

2008

received

2007-08-08

revised

2007-11-09

accepted

2007-12-03

(unknown)

2008-01-24

Contributors

author

Anna Turska-Szewczuk

• Department of General Microbiology, Maria Curie-Sklodowska University, Lublin, Poland

author

Hubert Pietras

• Department of General Microbiology, Maria Curie-Sklodowska University, Lublin, Poland

author

Wojciech Borucki

• Department of Botany, Faculty of Agriculture and Biology, Warsaw Agricultural University, Warszawa, Poland

author

Ryszard Russa

• Department of General Microbiology, Maria Curie-Sklodowska University, Lublin, Poland

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