Preferences help
enabled [disable] Abstract
Number of results
2008 | 55 | 1 | 161-174
Article title

Redox state of plastoquionone pool regulates expression of Arabidopsis thaliana genes in response to elevated irradiance*

Title variants
Languages of publication
DNA microarray technology was applied to gain insight into the role of the redox state of PQ pool as a retrograde factor mediating differential expression of Arabidopsis nuclear genes during the acclimation to changing irradiance. DNA microarray chips containing probes corresponding to 24 000 Arabidopsis nuclear genes were screened with cRNA samples prepared from leaves of plants exposed for 5 h to low irradiance (control) vs. medium, high and excessive irradiances (MI, HI and EI, respectively). Six hundred and sixty three genes were differentially expressed as a result of an exposure to at least one elevated irradiance. Among 663 differentially expressed genes a total of 50 were reverted by DCMU - 24 ones modulated at medium irradiance, 32 ones modulated at high irradiance and a single one modulated at excessive irradiance. We postulate that their expression is regulated by redox state of plastoquinone (PQ) pool. Thus the PQ-mediated redox regulation of expression of Arabidopsis nuclear genes is probably limited to the irradiance window representing non-stressing conditions. We found that the promoter regions of the PQ-regulated genes contained conserved elements, suggesting transcriptional control by a shared set of trans-acting factors which participate in signal transduction from the redox state of the PQ pool.
Physical description
  • Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Laboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  • Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland
  • Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15: 63-78.
  • Allen JF (1995) Thylakoid protein phosphorylation, state 1 state 2 transitions, and photosystem stoichiometry adjustment: redox control at multiple levels of gene expression. Physiol Plant 93: 196-205.
  • Bailey S, Walters RG, Jansson S, Horton P (2001) Acclimation of Arabidopsis thaliana to the light environment: the existence of separate low light and high light responses. Planta 213: 794-801.
  • Ball L, Accotto GP, Bechtold U, Creissen G, Funck D, Jimenez A, Kular B, Leyland N, Mejia-Carranza J, Reynolds H, Karpinski S, Mullineaux PM (2004) Evidence for a direct link between glutathione biosynthesis and stress defense gene expression. Plant Cell 16: 2448-2462.
  • Bilger W, Schreiber U (1986) Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves. Photosynth Res 10: 303-308.
  • Borevitz JO, Xia Y, Blount J, Dixon RA, Lamba C (2000) Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell 12: 2383-2393.
  • Chen YB, Durnford DG, Koblizek M, Falkowski PG (2004) Plastid regulation of Lhcb1 transcription in the chlorophyte alga Dunaliella tertiolecta. Plant Physiol 136: 3737-3750.
  • Cheng L, Wong WH (2001a) Model-based analysis of oligonucleotide arrays: model validation, design issues and standard error application. Genome Biol 2: research032.1-0032.11.
  • Cheng L, Wong WH (2001b) Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection. Proc Natl Acad Sci USA 98: 31-36.
  • Escoubas JM, Lomas M, La Roche J, Falkowski PG (1995) Light intensity regulation of cab gene transcription is signaled by the redox state of plastoquinone pool. Proc Natl Acad Sci USA 92: 10237-10241.
  • Fey V, Wagner R, Brautigam K, Wirtz M, Hell R, Dietzmann A, Leister D, Oelmuller R, Pfannschmidt T (2005) Retrograde plastid redox signals in the expression of nuclear genes for chloroplast of Arabdiopsis thaliana. J Biol Chem 280: 5318-5328.
  • Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory elements (PLACE) database. Nucleic Acids Res 27: 297-300.
  • Hudson ME, Quail PH (2003) Identification of promoter motifs involved in the network of phytochrome A-regulated gene expression by combined analysis of genomic sequence and microarray data. Plant Physiol 133: 1605-1616.
  • Jackowski G, Olkiewicz P, Żelisko A (2003) The acclimative response of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHCII) to elevated irradiances at the level of trimeric subunits. J Photochem Photobiol B 70: 163-170.
  • Johanningmeier U, Howell SH (1984) Regulation of light-harvesting chlorophyll-binding protein mRNA accumulation in Chlamydomonas reinhardtii. Possible involvment of chlorophyll synthesis precursors. J Biol Chem 259: 13541-13549.
  • Karpiński S, Escobar C, Karpińska B, Creissen G, Mullineauxa PM (1997) Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. Plant Cell 9: 627-640.
  • Kimura M, Yamamoto YY, Seki M, Sakurai T, Sato M, Abe T, Yoshida S, Manabe K, Shinozaki K, Matsui M (2003) Identification of Arabidopsis genes regulated by high light-stress using cDNA microarray. Photochem Photobiol 77: 226-233.
  • Kropat J, Oster U, Rudiger W, Beck CF (1997) Chlorophyll precursors are signals of chloroplast origin involved in light induction of nuclear heat-shock genes. Proc Natl Acad Sci USA 94: 14168-14172.
  • Leong TY, Anderson JM (1987) Adaptation of the thylakoid membranes of pea chloroplasts to light intensities. I. Study on the distribution of chlorophyll-protein complexes. Photosynth Res 5: 117-128.
  • Menkens AE, Schindler U, Cashmore AR (1995) The G-box: a ubiquitous regulatory DNA element in plants bound by the GBF family of bZIP proteins. Trends Biochem Sci 20: 506-510.
  • Mochizuki N, Brusslan JA, Larkin R, Nagatani A, Chory J (2001) Arabidopsis genomes uncoupled 5 (GUN5) mutant reveals the involvement of Mg-chelatase H subunit in plastid-to-nucleus signal transduction. Proc Natl Acad Sci USA 98: 2053-2058.
  • Molina C, Grotewold E (2005) Genome wide analysis of Arabidopsis core promoters. BMC Genomics 6: 25-36.
  • Oster U, Brunner H, Rudiger W (1996) The greening process in cress seedlings. V. Possible interference of chlorophyll precursors, accumulated after thujaplicin treatment, with light-regulated expression of Lhc genes. J Photochem Photobiol B 36: 255-261.
  • Piippo M, Akllahverdiyeva Y, Paakkarinen V, Suoranta UM, Battchikova N, Aro EM (2006) Chloroplast-mediated regulation of nuclear genes in Arabidopsis thaliana in the absence of light stress. Physiol Genomics 25: 142-152.
  • Polle A (1996) Mehler reaction: friend or foe in photosynthesis. Bot Acta 109: 84-89.
  • Rossel JB, Wilson IW, Pogson BJ (2002) Global changes in gene expression in response to high light in Arabidopsis. Plant Physiol 130: 1109-1120.
  • Thijs G, Lescot M, Marchal K, Rombauts S, De Moor B, Rouze P, Moreau Y (2001) A higher order background model improves then detection of promoter regulatory elements by Gibbs sampling. Bioinformatics 18: 331-332.
  • Thijs G, Marchal K, Lescot M, Rombauts S, De Moor B, Rouze P, Moreau Y (2002) A Gibbs sampling method to detect overrepresented motifs in the upstream regions of coexpressed genes. J Comput Biol 9: 447-464.
  • Tohge T, Nishiyama MY, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K (2005) Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. Plant J 42: 218-235.
  • Vanderauwera S, Zimmermann P, Rombauts S, Vandenbeele S, Langebartels C, Gruissem W, Inze D, Van Breusegem F (2005) Genome-wide analysis of hydrogen peroxide-regulated gene expression in Arabidopsis revelas a high light-induced transcriptional cluster involved in anthocyanin biosynthesis. Plant Physiol 139: 806-821.
  • Yadav V, Kundu S, Chattopadhyay D, Negi P, Wei N, Deng XW, Chattopadhyay S (2002) Light regulated modulation of Z-box containing promoters by photoreceptors and downstream regulatory components, COP1 and HY5, in Arabidopsis. Plant J 31: 741-756.
  • Yang DH, Andersson B, Aro EM, Ohad I (2001) The redox state of plastoquinone pool controls the level of the light-harvesting chlorophyll a/b binding protein complex II (LHC II) during photoacclimation. Photosynth Res 68: 163-174.
  • Yoshinaga S, Kohjima M, Ogura K, Yokochi M, Noda Y, Sumimoto H, Inagaki F (2003) The PB1 domain and the PC motif-containing region are structurally similar protein binding modules. EMBO J 22: 4888-4897.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.