Metabolism of conjugated sterols in eggplant. Part 2. Phospholipid : steryl glucoside acyltransferase

• Authors: Anna Potocka , Jan Zimowski
• Languages of publication: EN

Abstracts

EN

A membrane-bound phospholipid : steryl glucoside acyltransferase from Solanum melongena leaves was partially purified and its specificity and molecular as well as kinetic properties were defined. Among the steryl glycosides tested (e.g. typical plant steryl glucosides, steryl galactosides and cholesteryl xyloside) the highest activity was found with cholesteryl glucoside, but some structurally related compounds such as sito- and stigmasteryl glucoside or galactoside as well as cholesteryl galactoside were also acylated, albeit at lower rates. The investigated enzyme was able to use all classes of phosphoglycerolipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol) as an acyl source for biosynthesis of acylated steryl glucoside. Among them 1,2-dimirystoylphosphatidylic acid appeared to be the best acyl donor. Apart from phosphoglycerolipids, 1,2-diacylglycerols were also used as acyl donor for steryl glucoside acylation, although at a distinctly lower rate. The acyl moiety was transferred from the C-1 position of phospholipid molecule. The investigated acyltrasferase activity was stimulated by 2-mercaptoethanol, Triton X-100, 1-monoacylglycerols and inhibited in the presence of divalent cations such as Ca2+, Mn2+, Zn2+ or Co2+, some lipids (MDGD, ceramide), detergents (Tween 20, 40, 60 and 80, Tyloxapol, sodium deoxycholate) and high ionic strength.

Keywords

EN

acylated steryl glucoside; Solanum melongena; steryl glucoside; phospholipid:steryl glucoside acyltransferase

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2008
• Volume: 55
• Issue: 1
• Pages: 135-140

Dates

published

2008

received

2007-03-05

revised

2007-11-09

accepted

2007-12-13

(unknown)

2008-01-16

Contributors

author

Anna Potocka

• Institute of Biochemistry, Warsaw University, Warszawa, Poland

author

Jan Zimowski

• Institute of Biochemistry, Warsaw University, Warszawa, Poland

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