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2008 | 55 | 1 | 119-126
Article title

Improved HPLC method for total plasma homocysteine detection and quantification

Content
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Languages of publication
EN
Abstracts
EN
Recent clinical research has pointed at hyperhomocysteinemia as an independent risk factor in a number of cardiovascular and neurological diseases. We have improved a chromatographic method of total plasma homocysteine measurements in order to obtain higher sensitivity, reliability and reproducibility. The method demonstrates excellent linearity (R = 0.999), range (< 2-100 µM), precision (instrumental RSD 0.06 and method RSD 1.17), accuracy (recovery of 99.92 and RSD 1.27), reproducibility, quantification limit and ruggedness (e.g. pH from 2.0 to 2.5). Because even a small increase in homocysteine level can be a significant risk factor of cardiovascular diseases, such a precise method is required. The constructed method allows the measurement of plasma pyridoxal phosphate, PLP, the co-enzyme form of vitamin B6, on the same column and similar reagents. The developed method has been successfully applied to measure both total plasma and serum homocysteine in a group of acute stroke patients.
Publisher

Year
Volume
55
Issue
1
Pages
119-126
Physical description
Dates
published
2008
received
2007-09-12
revised
2007-11-20
accepted
2007-12-11
(unknown)
2008-01-16
Contributors
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
  • Department of Neurology, Medical University of Gdańsk, Gdańsk, Poland
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
  • Department of Molecular Biology, University of Gdańsk, Gdańsk, Poland
author
  • Department of Neurology, Medical University of Gdańsk, Gdańsk, Poland
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, Gdańsk, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv55p119kz
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