Limitation of usage of PicoGreen dye in quantitative assays of double-stranded DNA in the presence of intercalating compounds

• Authors: Marcin Koba , Alicja Szostek , Jerzy Konopa
• Languages of publication: EN

Abstracts

EN

PicoGreen is a very sensitive fluorescent dye for quantitative assays of double-stranded DNA (dsDNA) in solution and is used in several analytical protocols in which sensitive and precise DNA detection is needed, also for examination of drug-DNA interactions. The data shown in this paper indicate that compounds intercalating to DNA influence the applicability of PicoGreen dye for quantitative measurements of dsDNA, and for this reason PicoGreen dye is not suitable for examination of drug-DNA interactions, especially interstrand DNA crosslinks.

Keywords

EN

quantitative assay; interstrand DNA cross-link determination; PicoGreen dye; double-stranded DNA; drug-DNA binding

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2007
• Volume: 54
• Issue: 4
• Pages: 883-886

Dates

published

2007

received

2007-09-09

revised

2007-11-20

accepted

2007-12-09

(unknown)

2007-12-10

Contributors

author

Marcin Koba

• Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, Gdańsk, Poland

author

Alicja Szostek

• Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, Gdańsk, Poland

author

Jerzy Konopa

• Department of Pharmaceutical Technology and Biochemistry, Gdańsk University of Technology, Gdańsk, Poland

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